{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T01:44:04Z","timestamp":1791423844965,"version":"4.3.3"},"reference-count":42,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2013,4,26]],"date-time":"2013-04-26T00:00:00Z","timestamp":1366934400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/www.springer.com\/tdm"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["BMC Infect Dis"],"published-print":{"date-parts":[[2013,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n          <jats:sec>\n            <jats:title>Background<\/jats:title>\n            <jats:p>\n              <jats:italic>Lactobacillus jensenii<\/jats:italic>, <jats:italic>L. iners<\/jats:italic>, <jats:italic>L. crispatus<\/jats:italic> and <jats:italic>L. gasseri<\/jats:italic> are the most frequently occurring lactobacilli in the vagina. However, the native species vary widely according to the studied population. The present study was performed to genetically determine the identity of <jats:italic>Lactobacillus<\/jats:italic> strains present in the vaginal discharge of healthy and bacterial vaginosis (BV) intermediate Mexican women.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Methods<\/jats:title>\n            <jats:p>In a prospective study, 31 strains preliminarily identified as <jats:italic>Lactobacillus<\/jats:italic> species were isolated from 21 samples collected from 105 non-pregnant Mexican women. The samples were classified into groups according to the Nugent score criteria proposed for detection of BV: normal (N), intermediate (I) and bacterial vaginosis (BV). We examined the isolates using culture-based methods as well as molecular analysis of the V1\u2013V3 regions of the 16S rRNA gene. Enterobacterial repetitive intergenic consensus (ERIC) sequence analysis was performed to reject clones.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Results<\/jats:title>\n            <jats:p>Clinical isolates (25\/31) were classified into four groups based on sequencing and analysis of the 16S rRNA gene: <jats:italic>L. acidophilus<\/jats:italic> (14\/25), <jats:italic>L. reuteri<\/jats:italic> (6\/25), <jats:italic>L. casei<\/jats:italic> (4\/25) and <jats:italic>L. buchneri<\/jats:italic> (1\/25). The remaining six isolates were presumptively identified as <jats:italic>Enterococcus<\/jats:italic> species. Within the <jats:italic>L. acidophilus<\/jats:italic> group, <jats:italic>L. gasseri<\/jats:italic> was the most frequently isolated species, followed by <jats:italic>L. jensenii<\/jats:italic> and <jats:italic>L. crispatus<\/jats:italic>. <jats:italic>L. fermentum<\/jats:italic>, <jats:italic>L. rhamnosus<\/jats:italic> and <jats:italic>L. brevis<\/jats:italic> were also isolated, and were placed in the <jats:italic>L. reuteri<\/jats:italic>, <jats:italic>L. casei<\/jats:italic> and <jats:italic>L. buchneri<\/jats:italic> groups, respectively. ERIC profile analysis showed intraspecific variability amongst the <jats:italic>L. gasseri<\/jats:italic> and <jats:italic>L. fermentum<\/jats:italic> species.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Conclusions<\/jats:title>\n            <jats:p>These findings agree with previous studies showing that <jats:italic>L. crispatus<\/jats:italic>, <jats:italic>L. gasseri<\/jats:italic> and <jats:italic>L. jensenii<\/jats:italic> are consistently present in the healthy vaginal ecosystem. Additional species or phylotypes were detected in the vaginal microbiota of the non-pregnant Mexican (Hispanic-mestizo) population, and thus, these results further our understanding of vaginal lactobacilli colonisation and richness in this particular population.<\/jats:p>\n          <\/jats:sec>","DOI":"10.1186\/1471-2334-13-189","type":"journal-article","created":{"date-parts":[[2013,4,26]],"date-time":"2013-04-26T00:15:00Z","timestamp":1366935300000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":42,"title":["Lactobacillusspecies isolated from vaginal secretions of healthy and bacterial vaginosis-intermediate Mexican women: a prospective study"],"prefix":"10.1186","volume":"13","author":[{"given":"Marcos Daniel","family":"Mart\u00ednez-Pe\u00f1a","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Graciela","family":"Castro-Escarpulli","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ma Guadalupe","family":"Aguilera-Arreola","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2013,4,26]]},"reference":[{"key":"2415_CR1","doi-asserted-by":"publisher","first-page":"371","DOI":"10.1146\/annurev-micro-092611-150157","volume":"66","author":"B Ma","year":"2012","unstructured":"Ma B, Forney LJ, Ravel J: Vaginal microbiome: rethinking health and disease. Annu Rev Microbiol. 2012, 66: 371-389. 10.1146\/annurev-micro-092611-150157.","journal-title":"Annu Rev Microbiol"},{"key":"2415_CR2","first-page":"171","volume":"43","author":"M Angeles-L\u00f3pez","year":"2001","unstructured":"Angeles-L\u00f3pez M, Garc\u00eda-Cano E, Aquino SC: Hydrogen peroxide production and resistance to nonoxinol-9 in Lactobacillus spp. isolated from the vagina of reproductive age women. Rev Latinoam Microbiol. 2001, 43: 171-176.","journal-title":"Rev Latinoam Microbiol"},{"key":"2415_CR3","doi-asserted-by":"publisher","first-page":"1950","DOI":"10.1086\/315109","volume":"180","author":"MA Antonio","year":"1999","unstructured":"Antonio MA, Hawes SE, Hillier SL: The identification of vaginal Lactobacillus species and the demographic and microbiologic characteristics of women colonized by these species. J Infect Dis. 1999, 180: 1950-1956. 10.1086\/315109.","journal-title":"J Infect Dis"},{"key":"2415_CR4","doi-asserted-by":"publisher","first-page":"1105","DOI":"10.1111\/j.1365-2672.2011.04977.x","volume":"110","author":"Y Turovskiy","year":"2011","unstructured":"Turovskiy Y, Sutyak Noll K, Chikindas ML: The aetiology of bacterial vaginosis. J Appl Microbiol. 2011, 110: 1105-1128. 10.1111\/j.1365-2672.2011.04977.x.","journal-title":"J Appl Microbiol"},{"key":"2415_CR5","doi-asserted-by":"publisher","first-page":"10","DOI":"10.1186\/1471-2334-11-10","volume":"11","author":"E Gillet","year":"2011","unstructured":"Gillet E, Meys JF, Verstraelen H, Bosire C, De Sutter P, Temmerman M, Broeck DV: Bacterial vaginosis is associated with uterine cervical human papillomavirus infection: a meta-analysis. BMC Infect Dis. 2011, 11: 10-10.1186\/1471-2334-11-10. [Published online]","journal-title":"BMC Infect Dis"},{"key":"2415_CR6","doi-asserted-by":"publisher","first-page":"121","DOI":"10.1038\/ismej.2007.12","volume":"1","author":"X Zhou","year":"2007","unstructured":"Zhou X, Brown CJ, Abdo Z, Davis CC, Hansmann MA, Joyce P, Foster JA, Forney LJ: Differences in the composition of vaginal microbial communities found in healthy Caucasian and black women. ISME J. 2007, 1: 121-133. 10.1038\/ismej.2007.12.","journal-title":"ISME J"},{"key":"2415_CR7","doi-asserted-by":"publisher","first-page":"2746","DOI":"10.1128\/JCM.40.8.2746-2749.2002","volume":"40","author":"A V\u00e1squez","year":"2002","unstructured":"V\u00e1squez A, Jakobsson T, Ahrn\u00e9 S, Forsum U, Molin G: Vaginal Lactobacillus flora of healthy Swedish women. J Clin Microbiol. 2002, 40: 2746-2749. 10.1128\/JCM.40.8.2746-2749.2002.","journal-title":"J Clin Microbiol"},{"key":"2415_CR8","first-page":"11","volume":"9","author":"A P\u00e9rez-Miravete","year":"1967","unstructured":"P\u00e9rez-Miravete A: Studies on the vaginal flora IX Classification of Lactobacilli of vaginal origin. Rev Latinoam Microbiol Parasitol. 1967, 9: 11-14.","journal-title":"Rev Latinoam Microbiol Parasitol"},{"key":"2415_CR9","doi-asserted-by":"publisher","first-page":"851485","DOI":"10.1155\/2011\/851485","volume":"2011","author":"C Hern\u00e1ndez-Rodr\u00edguez","year":"2011","unstructured":"Hern\u00e1ndez-Rodr\u00edguez C, Romero-Gonz\u00e1lez R, Albani-Campanario M, Figueroa-Dami\u00e1n R, Meraz-Cruz N, Hern\u00e1ndez-Guerrero C: Vaginal microbiota of healthy pregnant Mexican women is constituted by four Lactobacillus species and several vaginosis-associated bacteria. Infect Dis Obstet Gynecol. 2011, 2011: 851485-","journal-title":"Infect Dis Obstet Gynecol"},{"key":"2415_CR10","doi-asserted-by":"publisher","first-page":"969","DOI":"10.1046\/j.1365-2672.2000.01202.x","volume":"89","author":"A Nigatu","year":"2000","unstructured":"Nigatu A: Evaluation of numerical analyses of RAPD and API 50 CH patterns to differentiate Lactobacillus plantarum, L. fermentum, L. rhamnosus, L. sake, L. parabuchneri, L. gallinarum, L. casei, Weissella minor and related taxa isolated from kocho and tef. J Appl Microbiol. 2000, 89: 969-978. 10.1046\/j.1365-2672.2000.01202.x.","journal-title":"J Appl Microbiol"},{"key":"2415_CR11","doi-asserted-by":"publisher","first-page":"5309","DOI":"10.1128\/JCM.43.10.5309-5311.2005","volume":"43","author":"MA Boyd","year":"2005","unstructured":"Boyd MA, Antonio MA, Hillier SL: Comparison of API 50 CH strips to whole-chromosomal DNA probes for identification of Lactobacillus species. J Clin Microbiol. 2005, 43: 5309-5311. 10.1128\/JCM.43.10.5309-5311.2005.","journal-title":"J Clin Microbiol"},{"key":"2415_CR12","doi-asserted-by":"crossref","first-page":"750479","DOI":"10.1155\/2008\/750479","volume":"2008","author":"S Srinivasan","year":"2008","unstructured":"Srinivasan S, Fredricks DN: The human vaginal bacterial biota and bacterial vaginosis. Interdiscip Perspect Infect Dis. 2008, 2008: 750479-","journal-title":"Interdiscip Perspect Infect Dis"},{"issue":"5","key":"2415_CR13","doi-asserted-by":"publisher","first-page":"61","DOI":"10.1186\/1471-2180-5-61","volume":"14","author":"R Verhelst","year":"2005","unstructured":"Verhelst R, Verstraelen H, Claeys G, Verschraegen G, Van Simaey L, De Ganck C, De Backer E, Temmerman M, Vaneechoutte M: Comparison between Gram stain and culture for the characterization of vaginal microflora: definition of a distinct grade that resembles grade I microflora and revised categorization of grade I microflora. BMC Microbiol. 2005, 14 (5): 61-","journal-title":"BMC Microbiol"},{"issue":"11","key":"2415_CR14","doi-asserted-by":"publisher","first-page":"e28180","DOI":"10.1371\/journal.pone.0028180","volume":"6","author":"GL Santiago","year":"2011","unstructured":"Santiago GL, Cools P, Verstraelen H, Trog M, Missine G, El Aila N, Verhelst R, Tency I, Claeys G, Temmerman M, Vaneechoutte M: Longitudinal study of the dynamics of vaginal microflora during two consecutive menstrual cycles. PLoS One. 2011, 6 (11): e28180-10.1371\/journal.pone.0028180.","journal-title":"PLoS One"},{"key":"2415_CR15","doi-asserted-by":"crossref","first-page":"4264","DOI":"10.1128\/AEM.65.9.4264-4267.1999","volume":"65","author":"GW Tannock","year":"1999","unstructured":"Tannock GW, Tilsala-Timisjarvi A, Rodtong S, Ng J, Munro K, Alatossava T: Identification of Lactobacillus isolates from the gastrointestinal tract, silage, and yoghurt by 16S-23S rRNA gene intergenic spacer region sequence comparisons. Appl Environ Microbiol. 1999, 65: 4264-4267.","journal-title":"Appl Environ Microbiol"},{"key":"2415_CR16","first-page":"39","volume":"1","author":"DJ O'Sullivan","year":"2000","unstructured":"O'Sullivan DJ: Methods for analysis of the intestinal microflora. Curr Issues Intest Microbiol. 2000, 1: 39-50.","journal-title":"Curr Issues Intest Microbiol"},{"key":"2415_CR17","doi-asserted-by":"publisher","first-page":"4680","DOI":"10.1073\/pnas.1002611107","volume":"108","author":"J Ravel","year":"2011","unstructured":"Ravel J, Gajer P, Abdo Z, Schneider GM, Koenig SS, McCulle SL, Karlebach S, Gorle R, Russell J, Tacket CO, Brotman RM, Davis CC, Ault K, Peralta L, Forney LJ: Vaginal microbiome of reproductive-age women. Proc Natl Acad Sci USA. 2011, 108: 4680-4687. 10.1073\/pnas.1002611107.","journal-title":"Proc Natl Acad Sci USA"},{"key":"2415_CR18","doi-asserted-by":"publisher","first-page":"116","DOI":"10.1186\/1471-2180-9-116","volume":"9","author":"H Verstraelen","year":"2009","unstructured":"Verstraelen H, Verhelst R, Claeys G, De Backer E, Temmerman M, Vaneechoutte M: Longitudinal analysis of the vaginal microflora in pregnancy suggests that L. crispatus promotes the stability of the normal vaginal microflora and that L. gasseri and\/or L. iners are more conducive to the occurrence of abnormal vaginal microflora. BMC Microbiol. 2009, 9: 116-10.1186\/1471-2180-9-116.","journal-title":"BMC Microbiol"},{"key":"2415_CR19","first-page":"27","volume":"7","author":"F Hern\u00e1ndez-Mart\u00ednez","year":"2013","unstructured":"Hern\u00e1ndez-Mart\u00ednez F, Hern\u00e1ndez-Garc\u00eda JA, Mart\u00ednez-Pe\u00f1a MD, Mu\u00f1iz-Becerril BL, Hern\u00e1ndez-Cortez C, Castro-Escarpulli G, Aguilera-Arreola MG: Aetiology and frecuency of cervico-vaginal infections among Mexican women. AJMR. 2013, 7: 27-34.","journal-title":"AJMR"},{"key":"2415_CR20","doi-asserted-by":"crossref","first-page":"297","DOI":"10.1128\/jcm.29.2.297-301.1991","volume":"29","author":"RP Nugent","year":"1991","unstructured":"Nugent RP, Krohn MA, Hillier SL: Reliability of diagnosing bacterial vaginosis is improved by a standardized method of gram stain interpretation. J Clin Microbiol. 1991, 29: 297-301.","journal-title":"J Clin Microbiol"},{"key":"2415_CR21","doi-asserted-by":"publisher","first-page":"482","DOI":"10.1099\/00222615-44-6-482","volume":"44","author":"J Vila","year":"1996","unstructured":"Vila J, Marcos MA, Jimenez de Anta MT: A comparative study of different PCR-based DNA fingerprinting techniques for typing of the Acinetobacter calcoaceticus-A. baumannii complex. J Med Microbiol. 1996, 44: 482-489. 10.1099\/00222615-44-6-482.","journal-title":"J Med Microbiol"},{"key":"2415_CR22","doi-asserted-by":"crossref","first-page":"1671","DOI":"10.1128\/jcm.35.7.1671-1674.1997","volume":"45","author":"N Borrell","year":"1997","unstructured":"Borrell N, Acinas SG, Figueras MJ, Mart\u00ednez-Murcia AJ: Identification of Aeromonas clinical isolates by restriction fragment length polymorphism of PCR-amplified 16S rRNA genes. J Clin Microbiol. 1997, 45: 1671-1674.","journal-title":"J Clin Microbiol"},{"key":"2415_CR23","doi-asserted-by":"publisher","first-page":"221","DOI":"10.1093\/molbev\/msp259","volume":"27","author":"M Gouy","year":"2010","unstructured":"Gouy M, Guindon S, Gascuel O: SeaView version 4: a multiplatform graphical user interface for sequence alignment and phylogenetic tree building. Mol Biol Evol. 2010, 27: 221-224. 10.1093\/molbev\/msp259.","journal-title":"Mol Biol Evol"},{"key":"2415_CR24","doi-asserted-by":"publisher","first-page":"2947","DOI":"10.1093\/bioinformatics\/btm404","volume":"23","author":"MA Larkin","year":"2007","unstructured":"Larkin MA, Blackshields G, Brown NP, Chenna R, McGettigan PA, McWilliam H, Valentin F, Wallace IM, Wilm A, L\u00f3pez R, Thompson JD, Gibson FJ, Higgins DG: Clustal W and clustal X version 2 0. Bioinformatics. 2007, 23: 2947-2948. 10.1093\/bioinformatics\/btm404.","journal-title":"Bioinformatics"},{"key":"2415_CR25","first-page":"543","volume":"12","author":"N Galtier","year":"1996","unstructured":"Galtier N, Gouy M, Gautier C: SEAVIEW and PHYLO_WIN: two graphic tools for sequence alignment and molecular phylogeny. Comput Applic Biosci. 1996, 12: 543-548.","journal-title":"Comput Applic Biosci"},{"key":"2415_CR26","doi-asserted-by":"publisher","first-page":"1596","DOI":"10.1093\/molbev\/msm092","volume":"24","author":"K Tamura","year":"2007","unstructured":"Tamura K, Dudley J, Nei M, Kumar S: MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4 0. Mol Biol Evol. 2007, 24: 1596-1599. 10.1093\/molbev\/msm092.","journal-title":"Mol Biol Evol"},{"key":"2415_CR27","doi-asserted-by":"publisher","first-page":"39","DOI":"10.1111\/j.1574-6976.2001.tb00571.x","volume":"25","author":"R Rossell\u00f3-Mora","year":"2001","unstructured":"Rossell\u00f3-Mora R, Amann R: The species concept for prokaryotes. FEMS Microbiol Rev. 2001, 25: 39-67.","journal-title":"FEMS Microbiol Rev"},{"key":"2415_CR28","doi-asserted-by":"publisher","first-page":"1757","DOI":"10.1093\/bioinformatics\/btn322","volume":"24","author":"A Morgulis","year":"2008","unstructured":"Morgulis A, Coulouris G, Raytselis Y, Madden TL, Agarwala R, Sch\u00e4ffer AA: Database indexing for production megablast searches. Bioinformatics. 2008, 24: 1757-1764. 10.1093\/bioinformatics\/btn322.","journal-title":"Bioinformatics"},{"key":"2415_CR29","volume-title":"Lactobacillus Molecular Biology: From Genomics to Probiotics","author":"A Ljungh","year":"2009","unstructured":"Ljungh A, Wadstr\u00f6m T: Lactobacillus Molecular Biology: From Genomics to Probiotics. 2009, Norfolk, UK: Caister Academic Press"},{"key":"2415_CR30","doi-asserted-by":"publisher","first-page":"394","DOI":"10.1086\/430926","volume":"192","author":"MA Antonio","year":"2005","unstructured":"Antonio MA, Rabe LK, Hillier SL: Colonization of the rectum by Lactobacillus species and decreased risk of bacterial vaginosis. J Infect Dis. 2005, 192: 394-398. 10.1086\/430926.","journal-title":"J Infect Dis"},{"key":"2415_CR31","doi-asserted-by":"publisher","first-page":"23","DOI":"10.1016\/j.clinmicnews.2008.01.006","volume":"30","author":"CM Slover","year":"2008","unstructured":"Slover CM, Danzinger L: Lactobacillus: a review. Clin Microbiol Newsl. 2008, 30: 23-27. 10.1016\/j.clinmicnews.2008.01.006.","journal-title":"Clin Microbiol Newsl"},{"key":"2415_CR32","doi-asserted-by":"publisher","first-page":"451","DOI":"10.1046\/j.1365-2672.2002.01547.x","volume":"92","author":"SI Pavlova","year":"2002","unstructured":"Pavlova SI, Kilic AO, Kilic SS, So JS, Nader-Macias ME, Simoes JA, Tao L: Genetic diversity of vaginal lactobacilli from women in different countries based on 16S rRNA gene sequences. J Appl Microbiol. 2002, 92: 451-459. 10.1046\/j.1365-2672.2002.01547.x.","journal-title":"J Appl Microbiol"},{"key":"2415_CR33","doi-asserted-by":"publisher","first-page":"217","DOI":"10.1099\/00207713-49-1-217","volume":"49","author":"E Falsen","year":"1999","unstructured":"Falsen E, Pascual C, Sj\u00f6d\u00e9n B, Ohl\u00e9n M, Collins MD: Phenotypic and phylogenetic characterization of a novel Lactobacillus species from human sources: description of Lactobacillus iners sp nov. Int J Syst Bacteriol. 1999, 49: 217-221. 10.1099\/00207713-49-1-217.","journal-title":"Int J Syst Bacteriol"},{"key":"2415_CR34","doi-asserted-by":"publisher","first-page":"93","DOI":"10.1016\/j.ejogrb.2011.10.002","volume":"160","author":"L Petricevic","year":"2012","unstructured":"Petricevic L, Domig KJ, Nierscher FJ, Krondorfer I, Janitschek C, Kneifel W, Kiss H: Characterisation of the oral, vaginal and rectal Lactobacillus flora in healthy pregnant and postmenopausal women. Eur J Obstet Gynecol Reprod Biol. 2012, 160: 93-99. 10.1016\/j.ejogrb.2011.10.002.","journal-title":"Eur J Obstet Gynecol Reprod Biol"},{"key":"2415_CR35","first-page":"469","volume":"41","author":"SP Dimitonova","year":"2008","unstructured":"Dimitonova SP, Bakalov BV, Aleksandrova-Georgieva RN, Danova ST: Phenotypic and molecular identification of lactobacilli isolated from vaginal secretions. J Microbiol Immunol Infect. 2008, 41: 469-477.","journal-title":"J Microbiol Immunol Infect"},{"key":"2415_CR36","doi-asserted-by":"publisher","first-page":"59","DOI":"10.1097\/01.olq.0000175367.15559.c4","volume":"33","author":"KC Anukam","year":"2006","unstructured":"Anukam KC, Osazuwa EO, Ahonkhai I, Reid G: Lactobacillus vaginal microbiota of women attending a reproductive health care service in Benin city, Nigeria. Sex Transm Dis. 2006, 33: 59-62. 10.1097\/01.olq.0000175367.15559.c4.","journal-title":"Sex Transm Dis"},{"key":"2415_CR37","doi-asserted-by":"publisher","first-page":"83","DOI":"10.1186\/1471-2180-12-83","volume":"12","author":"V Jespers","year":"2012","unstructured":"Jespers V, Menten J, Smet H, Porados\u00fa S, Abdellati S, Verhelst R, Hardy L, Buv\u00e9 A, Crucitti T: Quantification of bacterial species of the vaginal microbiome in different groups of women, using nucleic acid amplification tests. BMC Microbiol. 2012, 12: 83-10.1186\/1471-2180-12-83.","journal-title":"BMC Microbiol"},{"key":"2415_CR38","doi-asserted-by":"publisher","first-page":"169","DOI":"10.1111\/j.1574-695X.2009.00618.x","volume":"58","author":"X Zhou","year":"2010","unstructured":"Zhou X, Hansmann MA, Davis CC, Suzuki H, Brown CJ, Sch\u00fctte U, Pierson JD, Forney LJ: The vaginal bacterial communities of Japanese women resemble those of women in other racial groups. FEMS Immunol Med Microbiol. 2010, 58: 169-181. 10.1111\/j.1574-695X.2009.00618.x.","journal-title":"FEMS Immunol Med Microbiol"},{"key":"2415_CR39","doi-asserted-by":"publisher","first-page":"115","DOI":"10.1186\/1471-2180-7-115","volume":"7","author":"E De Backer","year":"2007","unstructured":"De Backer E, Verhelst R, Verstraelen H, Alqumber MA, Burton JP, Tagg JR, Temmerman M, Vaneechoutte M: Quantitative determination by real-time PCR of four vaginal Lactobacillus species, Gardnerella vaginalis and Atopobium vaginae indicates an inverse relationship between L. gasseri and L. iners. BMC Microbiol. 2007, 7: 115-10.1186\/1471-2180-7-115.","journal-title":"BMC Microbiol"},{"key":"2415_CR40","doi-asserted-by":"publisher","first-page":"1899","DOI":"10.1056\/NEJMoa043802","volume":"353","author":"DN Fredricks","year":"2005","unstructured":"Fredricks DN, Fiedler TL, Marrazzo JM: Molecular identification of bacteria associated with bacterial vaginosis. N Engl J Med. 2005, 353: 1899-1911. 10.1056\/NEJMoa043802.","journal-title":"N Engl J Med"},{"key":"2415_CR41","doi-asserted-by":"publisher","first-page":"6764","DOI":"10.1128\/AEM.01150-09","volume":"75","author":"DP Stephenson","year":"2009","unstructured":"Stephenson DP, Moore RJ, Allison GE: Comparison and utilization of repetitive-blement PCR techniques for typing Lactobacillus isolates from the chicken gastrointestinal tract. Appl Environ Microbiol. 2009, 75: 6764-6776. 10.1128\/AEM.01150-09.","journal-title":"Appl Environ Microbiol"},{"key":"2415_CR42","doi-asserted-by":"publisher","first-page":"378","DOI":"10.1128\/AEM.00973-10","volume":"77","author":"GT Spear","year":"2011","unstructured":"Spear GT, Gilbert D, Landay AL, Zariffard R, French AL, Patel P, Gillevet PM: Pyrosequencing of the genital microbiotas of HIV-seropositive and -seronegative women reveals Lactobacillus iners as the predominant Lactobacillus species. Appl Environ Microbiol. 2011, 77: 378-381. 10.1128\/AEM.00973-10.","journal-title":"Appl Environ Microbiol"}],"container-title":["BMC Infectious Diseases"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1186\/1471-2334-13-189.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/article\/10.1186\/1471-2334-13-189\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1186\/1471-2334-13-189","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1186\/1471-2334-13-189.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,9,1]],"date-time":"2021-09-01T23:46:52Z","timestamp":1630540012000},"score":1,"resource":{"primary":{"URL":"https:\/\/bmcinfectdis.biomedcentral.com\/articles\/10.1186\/1471-2334-13-189"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,4,26]]},"references-count":42,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2013,12]]}},"alternative-id":["2415"],"URL":"https:\/\/doi.org\/10.1186\/1471-2334-13-189","relation":{},"ISSN":["1471-2334"],"issn-type":[{"value":"1471-2334","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,4,26]]},"assertion":[{"value":"25 August 2012","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"19 April 2013","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"26 April 2013","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}}],"article-number":"189"}}