{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,4,29]],"date-time":"2025-04-29T18:10:05Z","timestamp":1745950205468,"version":"3.40.4"},"reference-count":50,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2013,1,18]],"date-time":"2013-01-18T00:00:00Z","timestamp":1358467200000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/www.springer.com\/tdm"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["BMC Infect Dis"],"published-print":{"date-parts":[[2013,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>In April 2009, public health surveillance detected an increased number of influenza-like illnesses in Mexico City\u2019s hospitals. The etiological agent was subsequently determined to be a spread of a worldwide novel influenza A (H1N1) triple reassortant. The purpose of the present study was to demonstrate that molecular detection of pandemic influenza A (H1N1) 2009 strains is possible in archival material such as paraffin-embedded lung samples.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>In order to detect A (H1N1) virus sequences in archived biological samples, eight paraffin-embedded lung samples from patients who died of pneumonia and respiratory failure were tested for influenza A (H1N1) Neuraminidase (NA) RNA using<jats:italic>in situ<\/jats:italic>RT-PCR.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>We detected NA transcripts in 100% of the previously diagnosed A (H1N1)-positive samples as a cytoplasmic signal. No expression was detected by<jats:italic>in situ<\/jats:italic>RT-PCR in two Influenza-like Illness A (H1N1)-negative patients using standard protocols nor in a non-related cervical cell line.<jats:italic>In situ<\/jats:italic>relative transcription levels correlated with those obtained when<jats:italic>in vitro<\/jats:italic>RT-PCR assays were performed. Partial sequences of the NA gene from A (H1N1)-positive patients were obtained by the<jats:italic>in situ<\/jats:italic>RT-PCR-sequencing method. Sequence analysis showed 98% similarity with influenza viruses reported previously in other places.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>We have successfully amplified specific influenza A (H1N1) NA sequences using stored clinical material; results suggest that this strategy could be useful when clinical RNA samples are quantity limited, or when poor quality is obtained. Here, we provide a very sensitive method that specifically detects the neuraminidase viral RNA in lung samples from patients who died from pneumonia caused by Influenza A (H1N1) outbreak in Mexico City.<\/jats:p><\/jats:sec>","DOI":"10.1186\/1471-2334-13-20","type":"journal-article","created":{"date-parts":[[2013,1,18]],"date-time":"2013-01-18T07:14:31Z","timestamp":1358493271000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["In situ molecular identification of the Influenza A (H1N1) 2009 Neuraminidase in patients with severe and fatal infections during a pandemic in Mexico City"],"prefix":"10.1186","volume":"13","author":[{"given":"Rodolfo","family":"Ocadiz-Delgado","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Martha Estela","family":"Albino-Sanchez","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Enrique","family":"Garcia-Villa","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria Guadalupe","family":"Aguilar-Gonzalez","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carlos","family":"Cabello","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dora","family":"Rosete","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fidencio","family":"Mejia","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria Eugenia","family":"Manjarrez-Zavala","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carmen","family":"Ondarza-Aguilera","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rosa Ma","family":"Rivera-Rosales","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Patricio","family":"Gariglio","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2013,1,18]]},"reference":[{"key":"2194_CR1","doi-asserted-by":"publisher","first-page":"680","DOI":"10.1056\/NEJMoa0904252","volume":"361","author":"R Perez-Padilla","year":"2009","unstructured":"Perez-Padilla R, de la Rosa-Zamboni D, Ponce de Leon S, Hernandez M, Qui\u00f1ones-Falconi F, Bautista E, Ramirez-Venegas A, Rojas-Serrano J, Ormsby CE, Corrales A, Higuera A, Mondragon E, Cordova-Villalobos JA: INER Working Group on Influenza. Pneumonia and respiratory failure from swine-origin influenza A(H1N1) in Mexico. N Engl J Med. 2009, 361: 680-689. 10.1056\/NEJMoa0904252. [PubMed: 19564631]","journal-title":"N Engl J Med"},{"key":"2194_CR2","doi-asserted-by":"publisher","first-page":"1557","DOI":"10.1126\/science.1176062","volume":"324","author":"C Fraser","year":"2009","unstructured":"Fraser C, Donnelly CA, Cauchemez S, Hanage WP, Van Kerkhove MD, Hollingsworth TD, Griffin J, Baggaley RF, Jenkins HE, Lyons EJ, Jombart T, Hinsley WR, Grassly NC, Balloux F, Ghani AC, Ferguson NM, Rambaut A, Pybus OG, Lopez-Gatell H, Alpuche-Aranda CM, Chapela IB, Zavala EP, Guevara DM, Checchi F, Garcia E, Hugonnet S, Roth C: WHO Rapid Pandemic Assessment Collaboration. Pandemic potential of a strain of influenza A (H1N1): early findings. Science. 2009, 324: 1557-1561. 10.1126\/science.1176062. [PubMed: 19433588]","journal-title":"Science"},{"key":"2194_CR3","doi-asserted-by":"publisher","first-page":"643","DOI":"10.1016\/j.arcmed.2009.10.007","volume":"40","author":"CF Arias","year":"2009","unstructured":"Arias CF, Escalera-Zamudio M, Soto-Del R\u00edo Mde L, Cobi\u00e1n-G\u00fcemes AG, Isa P, L\u00f3pez S: Molecular anatomy of 2009 influenza virus A (H1N1). Arch Med Res. 2009, 40: 643-654. 10.1016\/j.arcmed.2009.10.007. [PubMed: 20304251]","journal-title":"Arch Med Res"},{"key":"2194_CR4","doi-asserted-by":"publisher","first-page":"e13256","DOI":"10.1371\/journal.pone.0013256","volume":"5","author":"HM Zepeda-Lopez","year":"2010","unstructured":"Zepeda-Lopez HM, Perea-Araujo L, Miliar-Garc\u00eda A, Dominguez-L\u00f3pez A, Xoconostle-C\u00e1zarez B, Lara-Padilla E, Ram\u00edrez Hernandez JA, Sevilla-Reyes E, Orozco ME, Ahued-Ortega A, Villase\u00f1or-Ruiz I, Garcia-Cavazos RJ, Teran LM: Inside the outbreak of the 2009 influenza A (H1N1)v virus in Mexico. PLoS One. 2010, 5: e13256-10.1371\/journal.pone.0013256. [PubMed: 20949040]","journal-title":"PLoS One"},{"key":"2194_CR5","first-page":"1691","volume-title":"Fields Virology","author":"P Wright","year":"2006","unstructured":"Wright P, Neumann G, Kawaoka Y: Orthomyxoviruses. Fields Virology. Edited by: Knipe D, Howley P. 2006, Lippincott Williams & Wilkins, Philadelphia, 1691-1740. 5","edition":"5"},{"key":"2194_CR6","doi-asserted-by":"publisher","first-page":"147","DOI":"10.1016\/j.virusres.2009.05.010","volume":"143","author":"DP Nayak","year":"2009","unstructured":"Nayak DP, Balogun RA, Yamada H, Zhou ZH, Barman S: Influenza virus morphogenesis and budding. Virus Res. 2009, 143: 147-161. 10.1016\/j.virusres.2009.05.010. [PubMed: 19481124]","journal-title":"Virus Res"},{"key":"2194_CR7","doi-asserted-by":"publisher","first-page":"3048","DOI":"10.1128\/JCM.02386-07","volume":"46","author":"K Tsukamoto","year":"2008","unstructured":"Tsukamoto K, Ashizawa H, Nakanishi K, Kaji N, Suzuki K, Okamatsu M, Yamaguchi S, Mase M: Subtyping of avian influenza viruses H1 to H15 on the basis of hemagglutinin genes by PCR assay and molecular determination of pathogenic potential. J Clin Microbiol. 2008, 46: 3048-3055. 10.1128\/JCM.02386-07. [PubMed: 18596143]","journal-title":"J Clin Microbiol"},{"key":"2194_CR8","doi-asserted-by":"publisher","first-page":"4895","DOI":"10.1016\/j.vaccine.2010.05.031","volume":"28","author":"MP Girard","year":"2010","unstructured":"Girard MP, Tam JS, Assossou OM, Kieny MP: The 2009 A (H1N1) influenza virus pandemic: A review. Vaccine. 2010, 28: 4895-4902. 10.1016\/j.vaccine.2010.05.031. [PubMed: 20553769]","journal-title":"Vaccine"},{"key":"2194_CR9","doi-asserted-by":"publisher","first-page":"103","DOI":"10.1002\/msj.20164","volume":"77","author":"KA Swedish","year":"2010","unstructured":"Swedish KA, Conenello G, Factor SH: First Season of 2009 H1N1 Influenza. Mt Sinai J Med. 2010, 77: 103-113. 10.1002\/msj.20164. [PubMed: 20101715]","journal-title":"Mt Sinai J Med"},{"key":"2194_CR10","first-page":"453","volume":"58","author":"Centers for Disease Control and Prevention (CDC)","year":"2009","unstructured":"Centers for Disease Control and Prevention (CDC): Update: novel influenza A (H1N1) virus infections - worldwide, May 6, 2009. MMWR Morb Mortal Wkly Rep. 2009, 58: 453-458. [PubMed: 19444146]","journal-title":"MMWR Morb Mortal Wkly Rep"},{"key":"2194_CR11","doi-asserted-by":"publisher","first-page":"729","DOI":"10.1126\/science.1177373","volume":"326","author":"Y Yang","year":"2009","unstructured":"Yang Y, Sugimoto JD, Halloran ME, Basta NE, Chao DL, Matrajt L, Potter G, Kenah E, Longini IM: The transmissibility and control of pandemic influenza A (H1N1) virus. Science. 2009, 326: 729-733. 10.1126\/science.1177373. [PubMed: 19745114]","journal-title":"Science"},{"key":"2194_CR12","doi-asserted-by":"publisher","first-page":"S326","DOI":"10.1093\/cid\/cir047","volume":"4","author":"AM Caliendo","year":"2011","unstructured":"Caliendo AM: Multiplex PCR, and emerging technologies for the detection of respiratory pathogens. Clin Infect Dis. 2011, 4: S326-S330. [PubMed PMID: 21460291]","journal-title":"Clin Infect Dis"},{"issue":"2","key":"2194_CR13","doi-asserted-by":"publisher","first-page":"326","DOI":"10.1111\/j.1365-2559.2011.04081.x","volume":"60","author":"A Bal","year":"2012","unstructured":"Bal A, Suri V, Mishra B, Bhalla A, Agarwal R, Abrol A, Ratho RK, Joshi K: Pathology and virology findings in cases of fatal influenza A H1N1 virus infection in 2009\u20132010. Histopathology. 2012, 60 (2): 326-335. 10.1111\/j.1365-2559.2011.04081.x. [PubMed PMID: 22211291]","journal-title":"Histopathology"},{"issue":"2","key":"2194_CR14","doi-asserted-by":"publisher","first-page":"123","DOI":"10.1016\/j.jmoldx.2010.09.004","volume":"13","author":"AM Denison","year":"2011","unstructured":"Denison AM, Blau DM, Jost HA, Jones T, Rollin D, Gao R, Liu L, Bhatnagar J, Deleon-Carnes M, Shieh WJ, Paddock CD, Drew C, Adem P, Emery SL, Shu B, Wu KH, Batten B, Greer PW, Smith CS, Bartlett J, Montague JL, Patel M, Xu X, Lindstrom S, Klimov AI, Zaki SR: Diagnosis of influenza from respiratory autopsy tissues: detection of virus by real-time reverse transcription-PCR in 222 cases. J Mol Diagn. 2011, 13 (2): 123-128. 10.1016\/j.jmoldx.2010.09.004. [PubMed PMID: 21354045]","journal-title":"J Mol Diagn"},{"issue":"1","key":"2194_CR15","doi-asserted-by":"publisher","first-page":"24","DOI":"10.1016\/j.medin.2011.10.005","volume":"36","author":"N Nin","year":"2012","unstructured":"Nin N, S\u00e1nchez-Rodr\u00edguez C, Ver LS, Cardinal P, Ferruelo A, Soto L, Deicas A, Campos N, Rocha O, Ceraso DH, El-Assar M, Ort\u00edn J, Fern\u00e1ndez-Segoviano P, Esteban A, Lorente JA: Lung histopathological findings in fatal pandemic influenza A (H1N1). Med Intensiva. 2012, 36 (1): 24-31. 10.1016\/j.medin.2011.10.005. [PubMed PMID: 22154847]","journal-title":"Med Intensiva"},{"issue":"8","key":"2194_CR16","doi-asserted-by":"publisher","first-page":"655","DOI":"10.1016\/j.arcmed.2009.10.001","volume":"40","author":"J Guarner","year":"2009","unstructured":"Guarner J, Falc\u00f3n-Escobedo R: Comparison of the pathology caused by H1N1, H5N1, and H3N2 influenza viruses. Arch Med Res. 2009, 40 (8): 655-661. 10.1016\/j.arcmed.2009.10.001. [PubMed PMID: 20304252]","journal-title":"Arch Med Res"},{"key":"2194_CR17","doi-asserted-by":"publisher","first-page":"117","DOI":"10.1016\/j.anndiagpath.2006.02.003","volume":"10","author":"GJ Nuovo","year":"2006","unstructured":"Nuovo GJ: The surgical and cytopathology of viral infections: utility of immunohistochemistry, in situ hybridization, and in situ polymerase chain reaction amplification. Ann Diagn Pathol. 2006, 10: 117-131. 10.1016\/j.anndiagpath.2006.02.003. [PubMed PMID: 16546050]","journal-title":"Ann Diagn Pathol"},{"key":"2194_CR18","doi-asserted-by":"publisher","first-page":"914","DOI":"10.1097\/01.MP.0000024291.37651.CD","volume":"15","author":"AM Cioc","year":"2002","unstructured":"Cioc AM, Nuovo GJ: Histologic and in situ viral findings in the myocardium in cases of sudden, unexpected death. Mod Pathol. 2002, 15: 914-922. 10.1097\/01.MP.0000024291.37651.CD. [PubMed: 12218208]","journal-title":"Mod Pathol"},{"issue":"2","key":"2194_CR19","doi-asserted-by":"publisher","first-page":"227","DOI":"10.1309\/HV74-N24T-2K2C-3E8Q","volume":"114","author":"J Guarner","year":"2000","unstructured":"Guarner J, Shieh WJ, Dawson J, Subbarao K, Shaw M, Ferebee T, Morken T, Nolte KB, Freifeld A, Cox N, Zaki SR: Immunohistochemical and in situ hybridization studies of influenza A virus infection in human lungs. Am J Clin Pathol. 2000, 114 (2): 227-233. 10.1309\/HV74-N24T-2K2C-3E8Q. [PubMed PMID: 10941338]","journal-title":"Am J Clin Pathol"},{"key":"2194_CR20","first-page":"128","volume":"121","author":"C del Rio","year":"2010","unstructured":"del Rio C, Guarner J: The 2009 influenza A (H1N1) pandemic: what have we learned in the past 6 months. Trans Am Clin Climatol Assoc. 2010, 121: 128-137. discussion 138\u2013140 [PubMed PMID: 20697556]","journal-title":"Trans Am Clin Climatol Assoc"},{"key":"2194_CR21","doi-asserted-by":"publisher","first-page":"517","DOI":"10.1586\/eri.10.24","volume":"8","author":"R Wang","year":"2010","unstructured":"Wang R, Taubenberger JK: Methods for molecular surveillance of influenza. Expert Rev Anti Infect Ther. 2010, 8: 517-527. 10.1586\/eri.10.24. [PubMed PMID: 20455681]","journal-title":"Expert Rev Anti Infect Ther"},{"key":"2194_CR22","doi-asserted-by":"publisher","first-page":"113","DOI":"10.2174\/138920110790725339","volume":"11","author":"K Li","year":"2010","unstructured":"Li K, Xiao L, Liu C, Zhang J, Sirois P: Transforming viral sequences to A (H1N1) flu diagnosis: the current status and future prospects of rtPCR based assays. Curr Pharm Biotechnol. 2010, 11: 113-116. 10.2174\/138920110790725339. [PubMed PMID: 19929820]","journal-title":"Curr Pharm Biotechnol"},{"key":"2194_CR23","doi-asserted-by":"publisher","first-page":"155","DOI":"10.1007\/s10156-010-0035-Y","volume":"16","author":"H Takahashi","year":"2010","unstructured":"Takahashi H, Otsuka Y, Patterson BK: Diagnostic tests for influenza and other respiratory viruses: determining performance specifications based on clinical setting. J Infect Chemother. 2010, 16: 155-161. 10.1007\/s10156-010-0035-y. [PubMed PMID: 20191301]","journal-title":"J Infect Chemother"},{"key":"2194_CR24","doi-asserted-by":"publisher","first-page":"6946","DOI":"10.1158\/1078-0432.CCR-06-1214","volume":"12","author":"ME Manjarrez","year":"2006","unstructured":"Manjarrez ME, Ocadiz R, Valle L, Pacheco C, Marroquin A, De la Torre C, Selman M, Gariglio P: Detection of human papillomavirus and relevant tumor suppressors and oncoproteins in laryngeal tumors. Clin Cancer Res. 2006, 12: 6946-6951. 10.1158\/1078-0432.CCR-06-1214. [PubMed: 17145812]","journal-title":"Clin Cancer Res"},{"key":"2194_CR25","doi-asserted-by":"publisher","first-page":"513","DOI":"10.1007\/s11248-009-9243-6","volume":"18","author":"R Ocadiz-Delgado","year":"2009","unstructured":"Ocadiz-Delgado R, Marroquin-Chavira A, Hernandez-Mote R, Valencia C, Manjarrez-Zavala ME, Covarrubias L, Gariglio P: Induction of focal epithelial hyperplasia in tongue of young bk6-E6\/E7 HPV16 transgenic mice. Transgenic Res. 2009, 18: 513-527. 10.1007\/s11248-009-9243-6. [PubMed: 19165615]","journal-title":"Transgenic Res"},{"key":"2194_CR26","doi-asserted-by":"publisher","first-page":"77","DOI":"10.1186\/1743-422X-5-77","volume":"5","author":"AC Alvarez","year":"2008","unstructured":"Alvarez AC, Brunck ME, Boyd V, Lai R, Virtue E, Chen W, Bletchly C, Heine HG, Barnard R: A broad spectrum, one-step reverse-transcription PCR amplification of the neuraminidase gene from multiple subtypes of influenza A virus. Virol J. 2008, 5: 77-10.1186\/1743-422X-5-77. [PubMed: 18613963]","journal-title":"Virol J"},{"key":"2194_CR27","volume-title":"Eur J Cancer Prev","author":"I Leon-Galicia","year":"2012","unstructured":"Leon-Galicia I, Diaz-Chavez J, Garcia-Villa E, Uribe-Figueroa L, Hidalgo-Miranda A, Herrera LA, Alvarez-Rios E, Garcia-Mena J, Gariglio P: Resveratrol induces downregulation of DNA repair genes in MCF-7 human breast cancer cells. Eur J Cancer Prev. 2012, [Epub ahead of print] [PubMed PMID: 22644231]"},{"issue":"8","key":"2194_CR28","doi-asserted-by":"publisher","first-page":"3300","DOI":"10.1158\/0008-5472.CAN-08-2036","volume":"15;69","author":"L D\u00edaz","year":"2009","unstructured":"D\u00edaz L, Ceja-Ochoa I, Restrepo-Angulo I, Larrea F, Avila-Ch\u00e1vez E, Garc\u00eda-Becerra R, Borja-Cacho E, Barrera D, Ahumada E, Gariglio P, Alvarez-Rios E, Ocadiz-Delgado R, Garcia-Villa E, Hern\u00e1ndez-Gallegos E, Camacho-Arroyo I, Morales A, Ordaz-Rosado D, Garc\u00eda-Latorre E, Escamilla J, S\u00e1nchez-Pe\u00f1a LC, Saqui-Salces M, Gamboa-Dominguez A, Vera E, Uribe-Ram\u00edrez M, Murbarti\u00e1n J, Ortiz CS, Rivera-Guevara C, De Vizcaya-Ruiz A, Camacho J: Estrogens and human papilloma virus oncogenes regulate human ether-\u00e0-go-go-1 potassium channel expression. Cancer Res. 2009, 15;69 (8): 3300-3307. [PubMed PMID: 19351862]","journal-title":"Cancer Res"},{"issue":"2","key":"2194_CR29","doi-asserted-by":"publisher","first-page":"671","DOI":"10.1124\/jpet.103.051557","volume":"306","author":"A Ba\u00f1uelos","year":"2003","unstructured":"Ba\u00f1uelos A, Reyes E, Ocadiz R, Alvarez E, Moreno M, Monroy A, Gariglio P: Neocarzinostatin induces an effective p53-dependent response in human papillomavirus-positive cervical cancer cells. J Pharmacol Exp Ther. 2003, 306 (2): 671-680. 10.1124\/jpet.103.051557. [PubMed PMID: 12750435]","journal-title":"J Pharmacol Exp Ther"},{"key":"2194_CR30","doi-asserted-by":"publisher","first-page":"19","DOI":"10.1002\/dvg.20357","volume":"46","author":"R Ocadiz-Delgado","year":"2008","unstructured":"Ocadiz-Delgado R, Castaneda-Saucedo E, Indra AK, Hernandez-Pando R, Gariglio P: Impaired cervical homeostasis upon selective ablation of RXRalpha in epithelial cells. Genesis. 2008, 46: 19-28. 10.1002\/dvg.20357. [PubMed: 18196602]","journal-title":"Genesis"},{"key":"2194_CR31","doi-asserted-by":"publisher","first-page":"1329","DOI":"10.1177\/002215540104901101","volume":"49","author":"GJ Nuovo","year":"2001","unstructured":"Nuovo GJ: Co-labeling using in situ PCR: a review. J Histochem Cytochem. 2001, 49: 1329-1339. 10.1177\/002215540104901101. [PubMed: 11668186]","journal-title":"J Histochem Cytochem"},{"key":"2194_CR32","doi-asserted-by":"publisher","first-page":"402","DOI":"10.1006\/meth.2001.1262","volume":"25","author":"KJ Livak","year":"2001","unstructured":"Livak KJ, Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(\u2212Delta Delta C(T)) Method. Methods. 2001, 25: 402-408. 10.1006\/meth.2001.1262. [PubMed: 11846609]","journal-title":"Methods"},{"key":"2194_CR33","doi-asserted-by":"crossref","first-page":"1059","DOI":"10.18388\/abp.2004_3539","volume":"51","author":"A Yalcin","year":"2004","unstructured":"Yalcin A: Quantification of thioredoxin mRNA expression in the rat hippocampus by real-time PCR following oxidative stress. Acta Biochim Pol. 2004, 51: 1059-1065. [PubMed: 15625578]","journal-title":"Acta Biochim Pol"},{"key":"2194_CR34","doi-asserted-by":"publisher","first-page":"S98","DOI":"10.1086\/507554","volume":"194","author":"M Petric","year":"2006","unstructured":"Petric M, Comanor L, Petti CA: Role of the laboratory in diagnosis of influenza during seasonal epidemics and potential pandemics. J Infect Dis. 2006, 194: S98-S110. 10.1086\/507554. [PubMed: 17163396]","journal-title":"J Infect Dis"},{"issue":"7","key":"2194_CR35","doi-asserted-by":"publisher","first-page":"e38665","DOI":"10.1371\/journal.pone.0038665","volume":"7","author":"J Xu","year":"2012","unstructured":"Xu J, Davis CT, Christman MC, Rivailler P, Zhong H, Donis RO, Lu G: Evolutionary History and Phylodynamics of Influenza A and B Neuraminidase (NA) Genes Inferred from Large-Scale Sequence Analyses. PLoS One. 2012, 7 (7): e38665-10.1371\/journal.pone.0038665. [PubMed PMID: 22808012]","journal-title":"PLoS One"},{"key":"2194_CR36","doi-asserted-by":"publisher","first-page":"S12","DOI":"10.1111\/j.1440-1843.2006.00800.x","volume":"Suppl","author":"RJ Mason","year":"2006","unstructured":"Mason RJ: Biology of alveolar type II cells. Respirology. 2006, Suppl: S12-S15. [PubMed: 16423262]","journal-title":"Respirology"},{"key":"2194_CR37","doi-asserted-by":"publisher","first-page":"669","DOI":"10.1146\/annurev.physiol.65.092101.142446","volume":"65","author":"MC Williams","year":"2003","unstructured":"Williams MC: Alveolar type I cells: molecular phenotype and development. Annu Rev Physiol. 2003, 65: 669-695. 10.1146\/annurev.physiol.65.092101.142446. [PubMed: 12428023]","journal-title":"Annu Rev Physiol"},{"key":"2194_CR38","doi-asserted-by":"publisher","first-page":"6844","DOI":"10.1128\/JVI.02200-10","volume":"85","author":"WC Yu","year":"2011","unstructured":"Yu WC, Chan RW, Wang J, Travanty EA, Nicholls JM, Peiris JS, Mason RJ, Chan MC: Viral replication and innate host responses in primary human alveolar epithelial cells and alveolar macrophages infected with influenza H5N1 and H1N1 viruses. J Virol. 2011, 85: 6844-6855. 10.1128\/JVI.02200-10. [PubMed: 21543489]","journal-title":"J Virol"},{"key":"2194_CR39","doi-asserted-by":"publisher","first-page":"661","DOI":"10.1165\/rcmb.2006-0410OC","volume":"36","author":"J Wang","year":"2007","unstructured":"Wang J, Edeen K, Manzer R, Chang Y, Wang S, Chen X, Funk CJ, Cosgrove GP, Fang X, Mason RJ: Differentiated human alveolar epithelial cells and reversibility of their phenotype in vitro. Am J Respir Cell Mol Biol. 2007, 36: 661-668. 10.1165\/rcmb.2006-0410OC. [PubMed: 17255555]","journal-title":"Am J Respir Cell Mol Biol"},{"key":"2194_CR40","doi-asserted-by":"publisher","first-page":"166","DOI":"10.2353\/ajpath.2010.100115","volume":"177","author":"WJ Shieh","year":"2010","unstructured":"Shieh WJ, Blau DM, Denison AM, Deleon-Carnes M, Adem P, Bhatnagar J, Sumner J, Liu L, Patel M, Batten B, Greer P, Jones T, Smith C, Bartlett J, Montague J, White E, Rollin D, Gao R, Seales C, Jost H, Metcalfe M, Goldsmith CS, Humphrey C, Schmitz A, Drew C, Paddock C, Uyeki TM, Zaki SR: 2009 pandemic influenza A (H1N1): pathology and pathogenesis of 100 fatal cases in the United States. Am J Pathol. 2010, 177: 166-175. 10.2353\/ajpath.2010.100115. [PubMed: 20508031]","journal-title":"Am J Pathol"},{"key":"2194_CR41","doi-asserted-by":"publisher","first-page":"499","DOI":"10.1146\/annurev.pathmechdis.3.121806.154316","volume":"3","author":"JK Taubenberger","year":"2008","unstructured":"Taubenberger JK, Morens DM: The pathology of influenza virus infections. Annu Rev Pathol. 2008, 3: 499-522. 10.1146\/annurev.pathmechdis.3.121806.154316. [PubMed: 18039138]","journal-title":"Annu Rev Pathol"},{"key":"2194_CR42","doi-asserted-by":"publisher","first-page":"1036","DOI":"10.3201\/eid1107.041313","volume":"11","author":"M Uiprasertkul","year":"2005","unstructured":"Uiprasertkul M, Puthavathana P, Sangsiriwut K, Pooruk P, Srisook K, Peiris M, Nicholls JM, Chokephaibulkit K, Vanprapar N, Auewarakul P: Influenza A H5N1 replication sites in humans. Emerg Infect Dis. 2005, 11: 1036-1041. 10.3201\/eid1107.041313. [PubMed: 16022777]","journal-title":"Emerg Infect Dis"},{"key":"2194_CR43","doi-asserted-by":"publisher","first-page":"147","DOI":"10.1038\/nm1529","volume":"13","author":"JM Nicholls","year":"2007","unstructured":"Nicholls JM, Chan MC, Chan WY, Wong HK, Cheung CY, Kwong DL, Wong MP, Chui WH, Poon LL, Tsao SW, Guan Y, Peiris JS: Tropism of avian influenza A (H5N1) in the upper and lower respiratory tract. Nat Med. 2007, 13: 147-149. 10.1038\/nm1529. [PubMed: 17206149]","journal-title":"Nat Med"},{"key":"2194_CR44","doi-asserted-by":"publisher","first-page":"617","DOI":"10.1016\/S0140-6736(04)15595-5","volume":"363","author":"JS Peiris","year":"2004","unstructured":"Peiris JS, Yu WC, Leung CW, Cheung CY, Ng WF, Nicholls JM, Ng TK, Chan KH, Lai ST, Lim WL, Yuen KY, Guan Y: Re-emergence of fatal human influenza A subtype H5N1 disease. Lancet. 2004, 363: 617-619. 10.1016\/S0140-6736(04)15595-5. [PubMed: 14987888]","journal-title":"Lancet"},{"key":"2194_CR45","doi-asserted-by":"publisher","first-page":"e1000115","DOI":"10.1371\/journal.ppat.1000115","volume":"4","author":"LA Perrone","year":"2008","unstructured":"Perrone LA, Plowden JK, Garc\u00eda-Sastre A, Katz JM, Tumpey TM: H5N1 and 1918 pandemic influenza virus infection results in early and excessive infiltration of macrophages and neutrophils in the lungs of mice. PLoS Pathog. 2008, 4: e1000115-10.1371\/journal.ppat.1000115. [PubMed: 18670648]","journal-title":"PLoS Pathog"},{"key":"2194_CR46","doi-asserted-by":"publisher","first-page":"227","DOI":"10.1007\/s00705-008-0277-5","volume":"154","author":"Y Chen","year":"2009","unstructured":"Chen Y, Deng W, Jia C, Dai X, Zhu H, Kong Q, Huang L, Liu Y, Ma C, Li J, Xiao C, Liu Y, Wei Q, Qin C: Pathological lesions and viral localization of influenza A (H5N1) virus in experimentally infected Chinese rhesus macaques: implications for pathogenesis and viral transmission. Arch Virol. 2009, 154: 227-233. 10.1007\/s00705-008-0277-5. [PubMed: 19130169]","journal-title":"Arch Virol"},{"key":"2194_CR47","doi-asserted-by":"publisher","first-page":"176","DOI":"10.1177\/104063870501700213","volume":"17","author":"K Jung","year":"2005","unstructured":"Jung K, Chae C: First outbreak of respiratory disease associated with swine influenza H1N2 virus in pigs in Korea. J Vet Diagn Invest. 2005, 17: 176-178. 10.1177\/104063870501700213. [PubMed: 15825500]","journal-title":"J Vet Diagn Invest"},{"key":"2194_CR48","doi-asserted-by":"publisher","first-page":"179","DOI":"10.1016\/j.jcpa.2004.09.008","volume":"132","author":"K Jung","year":"2005","unstructured":"Jung K, Ha Y, Chae C: Pathogenesis of swine influenza virus subtype H1N2 infection in pigs. J Comp Pathol. 2005, 132: 179-184. 10.1016\/j.jcpa.2004.09.008. [PubMed: 15737344]","journal-title":"J Comp Pathol"},{"key":"2194_CR49","doi-asserted-by":"publisher","first-page":"1137","DOI":"10.1016\/S0140-6736(07)61515-3","volume":"370","author":"J Gu","year":"2007","unstructured":"Gu J, Xie Z, Gao Z, Liu J, Korteweg C, Ye J, Lau LT, Lu J, Gao Z, Zhang B, McNutt MA, Lu M, Anderson VM, Gong E, Yu AC, Lipkin WI: H5N1 infection of the respiratory tract and beyond: a molecular pathology study. Lancet. 2007, 370: 1137-1145. 10.1016\/S0140-6736(07)61515-3. [PubMed: 17905166]","journal-title":"Lancet"},{"key":"2194_CR50","doi-asserted-by":"publisher","first-page":"1019","DOI":"10.1016\/S0029-7844(01)01625-8","volume":"98","author":"E Euscher","year":"2001","unstructured":"Euscher E, Davis J, Holzman I, Nuovo GJ: Coxsackie virus infection of the placenta associated with neurodevelopmental delays in the newborn. Obstet Gynecol. 2001, 98: 1019-1026. 10.1016\/S0029-7844(01)01625-8. [PubMed: 11755547]","journal-title":"Obstet Gynecol"}],"container-title":["BMC Infectious Diseases"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1186\/1471-2334-13-20.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/article\/10.1186\/1471-2334-13-20\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1186\/1471-2334-13-20","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1186\/1471-2334-13-20.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,29]],"date-time":"2025-04-29T17:36:37Z","timestamp":1745948197000},"score":1,"resource":{"primary":{"URL":"https:\/\/bmcinfectdis.biomedcentral.com\/articles\/10.1186\/1471-2334-13-20"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,1,18]]},"references-count":50,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2013,12]]}},"alternative-id":["2194"],"URL":"https:\/\/doi.org\/10.1186\/1471-2334-13-20","relation":{},"ISSN":["1471-2334"],"issn-type":[{"type":"electronic","value":"1471-2334"}],"subject":[],"published":{"date-parts":[[2013,1,18]]},"assertion":[{"value":"6 December 2011","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"10 January 2013","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"18 January 2013","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}}],"article-number":"20"}}