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In this study <jats:italic>in-vivo<\/jats:italic> induced antigens of this parasite was investigated using <jats:italic>in-vivo<\/jats:italic> induced antigen technology (IVIAT) and pooled sera from patients with serological evidence of acute infection.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Methods<\/jats:title>\n            <jats:p>The pooled sera was first pre-absorbed against three different preparations of antigens from <jats:italic>in-vitro<\/jats:italic>-grown cells of each <jats:italic>T. gondii<\/jats:italic> and <jats:italic>E. coli<\/jats:italic> XL1-Blue MRF\u2019, subsequently it was used to screen <jats:italic>T. gondii<\/jats:italic> cDNA phage expression library. Positive clones from each group were subjected to quantitative real-time PCR expression analysis on mRNA of <jats:italic>in-vivo<\/jats:italic> and <jats:italic>in-vitro<\/jats:italic> grown parasites.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Results<\/jats:title>\n            <jats:p>A total of 29 reactive clones from each IgM and IgG immunoscreenings were found to have high homology to <jats:italic>T. gondii<\/jats:italic> genes. Quantitative real-time PCR expression analysis showed that 20 IgM-detected genes and 11 IgG-detected genes were up-regulated <jats:italic>in-vivo<\/jats:italic> relative to their expression levels <jats:italic>in-vitro.<\/jats:italic> These included genes encoding micronemes, sterol-regulatory element binding protein site, SRS34A, MIC2-associated protein M2AP, nucleoredoxin, protein phosphatase 2C and several hypothetical proteins. A hypothetical protein (GenBank accession no. 7899266) detected by IgG had the highest <jats:italic>in-vivo<\/jats:italic> over <jats:italic>in-vitro<\/jats:italic> fold change of 499.86; while another up-regulated hypothetical protein (GenBank accession no. 7898829) recognized by IgM showed high sensitivity (90%) and moderate specificity (70%) in detecting <jats:italic>T. gondii<\/jats:italic> antibodies when tested with 20 individual serum samples.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Conclusion<\/jats:title>\n            <jats:p>The highly up-regulated genes and the corresponding proteins, in particular the hypothetical proteins, may be useful in further studies on understanding the disease pathogenesis and as potential vaccine candidates.<\/jats:p>\n          <\/jats:sec>","DOI":"10.1186\/1471-2334-13-287","type":"journal-article","created":{"date-parts":[[2013,6,24]],"date-time":"2013-06-24T08:14:34Z","timestamp":1372061674000},"update-policy":"http:\/\/dx.doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Identification and real-time expression analysis of selected Toxoplasma gondii in-vivoinduced antigens recognized by IgG and IgM in sera of acute toxoplasmosis patients"],"prefix":"10.1186","volume":"13","author":[{"given":"Atefeh","family":"Amerizadeh","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Boon Yin","family":"Khoo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ai Ying","family":"Teh","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Majid","family":"Golkar","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Izzati Zahidah","family":"Abdul Karim","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sabariah","family":"Osman","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Muhammad Hafiznur","family":"Yunus","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rahmah","family":"Noordin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2013,6,24]]},"reference":[{"key":"2493_CR1","doi-asserted-by":"publisher","first-page":"173","DOI":"10.1016\/j.protis.2006.02.005","volume":"157","author":"J Kope\u010dn\u00e1","year":"2006","unstructured":"Kope\u010dn\u00e1 J, Jirk\u016f M, Oborn\u00edk M, Tokarev YS, Luke\u0161 J, Modr\u1ef3 D: Phylogenetic analysis of coccidian parasites from invertebrates: search for missing links. 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