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We established another new rapid non-culture method for detection of <jats:italic>C. trachomatis<\/jats:italic> based on the measurement of \u03b1-mannosidase enzymatic activity in urogenital tract specimens.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Method<\/jats:title>\n            <jats:p>To evaluate the performance of this method, \u03b1-mannosidase activities of <jats:italic>C. trachomatis<\/jats:italic> serotype D strain \u3001 and 29 standard strains related to clinical urogenital pathogens were investigated. Furthermore, 553 urogenital tract specimens were used for clinical assays via cell culture method and ligase chain reaction method (LCR), adopting an expanded gold standard.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Results<\/jats:title>\n            <jats:p>Only <jats:italic>C. trachomatis<\/jats:italic> was positive for \u03b1-mannosidase activity among different types of microbes tested in the research. When prostate fluid specimens, which have some interfering activity, were excluded, the sensitivity and specificity of the enzymatic method were 91.8% (78\/85) and 98.3% (409\/416), respectively. There were no significant differences (<jats:italic>P<\/jats:italic> &gt; 0.05).<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Conclusions<\/jats:title>\n            <jats:p>These results showed that \u03b1-mannosidase activity could be utilised as a screening marker of <jats:italic>C. trachomatis<\/jats:italic> infection.<\/jats:p>\n          <\/jats:sec>","DOI":"10.1186\/1471-2334-13-36","type":"journal-article","created":{"date-parts":[[2013,1,24]],"date-time":"2013-01-24T17:14:22Z","timestamp":1359047662000},"update-policy":"http:\/\/dx.doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Rapid screening for Chlamydia trachomatis infection by detecting \u03b1-mannosidase activity in urogenital tract specimens"],"prefix":"10.1186","volume":"13","author":[{"given":"Ze-yu","family":"Wang","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guang-yu","family":"Fu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shan-mei","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dong-chun","family":"Qin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhong-quan","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jing","family":"Cui","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2013,1,24]]},"reference":[{"key":"2235_CR1","volume-title":"Sexually transmitted disease surveillance, 2009","author":"Centers for Disease Control and Prevention","year":"2011","unstructured":"Centers for Disease Control and Prevention: Sexually transmitted disease surveillance, 2009. 2011, Atlanta, GA: Centers for Disease Control and Prevention"},{"key":"2235_CR2","doi-asserted-by":"publisher","first-page":"650","DOI":"10.1002\/ijc.22325","volume":"120","author":"MM Madeleine","year":"2007","unstructured":"Madeleine MM, Anttila T, Schwartz SM, Saikku P, Leinonen M, Carter JJ, Wurscher M, Johnson LG, Galloway DA, Daling JR: Risk of cervical cancer associated with Chlamydia trachomatis antibodies by histology, HPV type and HPV cofactors. 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