{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,7]],"date-time":"2025-11-07T18:52:44Z","timestamp":1762541564297},"reference-count":25,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2006,7,21]],"date-time":"2006-07-21T00:00:00Z","timestamp":1153440000000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/www.springer.com\/tdm"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["BMC Infect Dis"],"published-print":{"date-parts":[[2006,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n          <jats:sec>\n            <jats:title>Background<\/jats:title>\n            <jats:p>Human monocytic ehrlichiosis (HME) and Rocky Mountain spotted fever (RMSF) are caused by <jats:italic>Ehrlichia chaffeensis<\/jats:italic> and <jats:italic>Rickettsia rickettsii<\/jats:italic>, respectively. The pathogenesis of RMSF relates to rickettsia-mediated vascular injury, but it is unclear in HME.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Methods<\/jats:title>\n            <jats:p>To study histopathologic responses in the lymphatic system for correlates of immune injury, lymph nodes from patients with HME (n = 6) and RMSF (n = 5) were examined. H&amp;E-stained lymph node tissues were examined for five histopathologic features, including hemophagocytosis, cellularity, necrosis, and vascular congestion and edema. The relative proportions of CD68 macrophages, CD8 and CD4 T lymphocytes, and CD20 B lymphocytes were evaluated by immunohistochemical staining.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Results<\/jats:title>\n            <jats:p>Hemophagocytosis was similar in HME and RMSF, and was greater than in control cases (p = .015). Cellularity in HME was not different from controls, whereas RMSF lymph nodes were markedly less cellular (p &lt; 0.002). <jats:italic>E. chaffeensis<\/jats:italic>-infected mononuclear phagocytes were infrequent compared to <jats:italic>R. rickettsii<\/jats:italic>-infected endothelial cells. More CD8 cells in lymph nodes were observed with HME (p &lt; .001), but no quantitative differences in CD4 lymphocytes, macrophages, or B lymphocytes were identified.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Conclusion<\/jats:title>\n            <jats:p>Hemophagocytosis, CD8 T cell expansion, and the paucity of infected cells in HME, suggest that <jats:italic>E. chaffeensis<\/jats:italic> infection leads to macrophage activation and immune-mediated injury.<\/jats:p>\n          <\/jats:sec>","DOI":"10.1186\/1471-2334-6-121","type":"journal-article","created":{"date-parts":[[2006,7,21]],"date-time":"2006-07-21T20:18:11Z","timestamp":1153513091000},"update-policy":"http:\/\/dx.doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":22,"title":["Lymph node hemophagocytosis in rickettsial diseases: a pathogenetic role for CD8 T lymphocytes in human monocytic ehrlichiosis (HME)?"],"prefix":"10.1186","volume":"6","author":[{"given":"Kerry L","family":"Dierberg","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J Stephen","family":"Dumler","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2006,7,21]]},"reference":[{"key":"385_CR1","doi-asserted-by":"publisher","first-page":"2145","DOI":"10.1099\/00207713-51-6-2145","volume":"51","author":"JS Dumler","year":"2001","unstructured":"Dumler JS, Barbet AF, Bekker CP, Dasch GA, Palmer GH, Ray SC, Rikihisa Y, Rurangirwa FR: Reorganization of genera in the families Rickettsiaceae and Anaplasmataceae in the order Rickettsiales : unification of some species of Ehrlichia with Anaplasma, Cowdria with Ehrlichia and Ehrlichia with Neorickettsia, descriptions of six new species combinations and designation of Ehrlichia equi and 'HGE agent' as subjective synonyms of Ehrlichia phagocytophila. 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