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Intracellular HCV RNA quantification of plus and minus strands was assessed before and after stimulation. PBMC from five healthy subjects were cultured for 7 days, flow cytometric analysis of DNA content was used to assess the mitogenic effect of SEA on PBMC proliferation compared to phytoheamaglutinine (PHA).<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Results<\/jats:title>\n            <jats:p>Quantification of the intracellular viral load showed increased copy number\/cell of both or either viral strands after induction with SEA in 18 of 26 patients (69.2%) thus indicating stimulation of viral replication. Flow cytometric analysis showed that mean \u00b1 S.D. of percent values of cell proliferation was induced from 3.2 \u00b1 1.5% in un-stimulated cells to 16.7 \u00b1 2.5 % and 16.84 \u00b1 1.7 % in cells stimulated with PHA and SEA respectively.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Conclusion<\/jats:title>\n            <jats:p>the present study supports earlier reports on SEA proliferative activity on PBMC and provides a strong evidence that the higher morbidity observed in patients co-infected with schistosomiasis and HCV is related, at least in part, to direct stimulation of viral replication by SEA.<\/jats:p>\n          <\/jats:sec>","DOI":"10.1186\/1471-2334-6-91","type":"journal-article","created":{"date-parts":[[2006,6,6]],"date-time":"2006-06-06T18:30:08Z","timestamp":1149618608000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["Soluble egg antigen of Schistosoma Haematobiuminduces HCV replication in PBMC from patients with chronic HCV infection"],"prefix":"10.1186","volume":"6","author":[{"given":"Mostafa K","family":"El-Awady","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Samar S","family":"Youssef","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Moataza H","family":"Omran","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ashraf A","family":"Tabll","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wael T","family":"El Garf","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ahmed M","family":"Salem","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2006,6,6]]},"reference":[{"key":"355_CR1","doi-asserted-by":"publisher","first-page":"427","DOI":"10.1016\/0140-6736(92)91508-6","volume":"340","author":"MA Kamel","year":"1992","unstructured":"Kamel MA, Ghaffar YA, Yasef MA, Wright M, Clark LC, Miller F: High HCV prevalence in Egyptian blood donors. 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