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We have assessed the behavior of mutants that lack two or three<jats:italic>rpf<\/jats:italic>genes in different combinations in our intraperitoneal model.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>C57Bl\/6 mice were intraperitonealy infected with H37Rv wild type<jats:italic>M. tuberculosis<\/jats:italic>or mutant strains that lacked two or three<jats:italic>rpf<\/jats:italic>genes in different combinations. After 90 days of infection aminoguanidine (AG) or anti-TNF\u03b1 antibodies were administrated. Organ bacillary loads were determined at various intervals post infection by plating serial dilutions of organ homogenates and enumerating bacteria.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>We found that the<jats:italic>rpf<\/jats:italic>triple and double mutants tested were attenuated in their ability to disseminate to mouse lungs after intraperitoneal administration and were defective in their ability to re-grow after immunosuppression induced by administration of aminoguanidine and anti-TNF\u03b1 antibodies.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Rpf proteins may have a significant physiological role for development of chronic TB infection and its reactivation<jats:italic>in vivo<\/jats:italic>.<\/jats:p><\/jats:sec>","DOI":"10.1186\/1471-2334-7-146","type":"journal-article","created":{"date-parts":[[2007,12,18]],"date-time":"2007-12-18T07:13:30Z","timestamp":1197962010000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":66,"title":["The role of resuscitation promoting factors in pathogenesis and reactivation of Mycobacterium tuberculosis during intra-peritoneal infection in mice"],"prefix":"10.1186","volume":"7","author":[{"given":"Sergey","family":"Biketov","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vasilii","family":"Potapov","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elena","family":"Ganina","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katrina","family":"Downing","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bavesh D","family":"Kana","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arseny","family":"Kaprelyants","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2007,12,17]]},"reference":[{"key":"589_CR1","doi-asserted-by":"publisher","first-page":"677","DOI":"10.1001\/jama.282.7.677","volume":"82","author":"C Dye","year":"1999","unstructured":"Dye C, Scheele S, Dolin P, Pathania V, Raviglione RC: Consensus statement. 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