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Previous association studies with populations from Europe and Asia have identified putative susceptibility markers for NSCL\/P in fibroblast growth factor 12 (<jats:italic>FGF12<\/jats:italic>), vinculin (<jats:italic>VCL<\/jats:italic>), connexin 43 (<jats:italic>CX43<\/jats:italic>) and in a region close to the ventral anterior homeobox 1 (<jats:italic>VAX1<\/jats:italic>) gene. However, there have thus far been no studies of these markers in NSCL\/P Brazilian patients, and as the genetic ancestry of the Brazilian population is highly varied, the predisposition to those disease markers can be different.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Herein we conducted a structured association study conditioned on the individual ancestry proportions to determine the role of 16 polymorphic markers within those genes in 300 patients with NSCL\/P and 385 unaffected controls.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>None of the alleles and genotypes showed association with NSCL\/P, though there was a significant association of the haplotype formed by<jats:italic>VAX1<\/jats:italic>rs10787760, rs6585429 and rs1871345 polymorphisms with NSCL\/P that did not persist Bonferroni correction for multiple tests.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Our results are consistent with a lack of involvement of<jats:italic>FGF12<\/jats:italic>,<jats:italic>VCL<\/jats:italic>and<jats:italic>CX43<\/jats:italic>variants with NSCL\/P pathogenesis in Brazilian patients. Furthermore, the higher frequency of a haplotype of<jats:italic>VAX1<\/jats:italic>with NSCL\/P patients suggests a low penetrant gene for oral cleft, and warrants further studies.<\/jats:p><\/jats:sec>","DOI":"10.1186\/1471-2350-14-53","type":"journal-article","created":{"date-parts":[[2013,5,16]],"date-time":"2013-05-16T14:16:01Z","timestamp":1368713761000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":23,"title":["Polymorphisms in FGF12, VCL, CX43 and VAX1in Brazilian patients with nonsyndromic cleft lip with or without cleft palate"],"prefix":"10.1186","volume":"14","author":[{"given":"Sibele Nascimento","family":"de Aquino","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ana Camila","family":"Messetti","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elizabete","family":"Bagordakis","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Herc\u00edlio","family":"Martelli-J\u00fanior","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mario Sergio Oliveira","family":"Swerts","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Edgard","family":"Graner","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ricardo D","family":"Coletta","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2013,5,16]]},"reference":[{"issue":"4","key":"1083_CR1","doi-asserted-by":"publisher","first-page":"314","DOI":"10.1590\/S1806-83242007000400006","volume":"21","author":"H Martelli-Junior","year":"2007","unstructured":"Martelli-Junior H, Porto LV, Martelli DR, Bonan PR, Freitas AB, Della Coletta R: Prevalence of nonsyndromic oral clefts in a reference hospital in the state of Minas Gerais, Brazil, between 2000\u20132005. 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