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We investigated the antitumor effect of lentivirus-mediated <jats:italic>kallistatin<\/jats:italic> gene transfer in a syngeneic murine tumor model.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Methods<\/jats:title>\n            <jats:p>Lentiviral vector encoding kallistatin (LV-Kallistatin) was constructed. The expression of kallistatin was verified by enzyme-linked immunosorbent assay (ELISA), and the bioactivity of kallistatin was determined by using cell proliferation, migration, and invasion assays. In addition, antitumor effects of LV-Kallistatin were evaluated by the intravenous injection of virus into tumor-bearing mice.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Results<\/jats:title>\n            <jats:p>The conditioned medium from LV-Kallistatin-treated cells inhibited the migration and proliferation of endothelial cells. Meanwhile, it also reduced the migration and invasion of tumor cells. In the experimental lung metastatic model, tumor-bearing mice receiving LV-Kallistatin had lower tumor nodules and longer survival than those receiving control virus or saline. Moreover, the microvessel densities, the levels of vascular endothelial growth factor (VEGF), tumor necrosis factor (TNF)-\u03b1, and nuclear factor \u03baB (NF-\u03baB) transcriptional activity were reduced in the LV-Kallistatin-treated mice.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Conclusion<\/jats:title>\n            <jats:p>Results of this study showed that systemic administration of lentiviral vectors encoding kallistatin inhibited the growth of metastatic tumor and prolonged the survival of tumor-bearing mice. These results suggest that gene therapy using lentiviruses carrying the <jats:italic>kallistatin<\/jats:italic> gene, which exerts anti-angiogenic and anti-inflammatory activities, represents a promising strategy for the treatment of lung cancer.<\/jats:p>\n          <\/jats:sec>","DOI":"10.1186\/1471-2407-10-245","type":"journal-article","created":{"date-parts":[[2010,6,1]],"date-time":"2010-06-01T06:14:11Z","timestamp":1275372851000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":30,"title":["Inhibition of experimental lung metastasis by systemic lentiviral delivery of kallistatin"],"prefix":"10.1186","volume":"10","author":[{"given":"Ai-Li","family":"Shiau","sequence":"first","affiliation":[]},{"given":"Min-Li","family":"Teo","sequence":"additional","affiliation":[]},{"given":"Shin-Yao","family":"Chen","sequence":"additional","affiliation":[]},{"given":"Chrong-Reen","family":"Wang","sequence":"additional","affiliation":[]},{"given":"Jeng-Long","family":"Hsieh","sequence":"additional","affiliation":[]},{"given":"Meng-Ya","family":"Chang","sequence":"additional","affiliation":[]},{"given":"Chih-Jui","family":"Chang","sequence":"additional","affiliation":[]},{"given":"Julie","family":"Chao","sequence":"additional","affiliation":[]},{"given":"Lee","family":"Chao","sequence":"additional","affiliation":[]},{"given":"Chao-Liang","family":"Wu","sequence":"additional","affiliation":[]},{"given":"Che-Hsin","family":"Lee","sequence":"additional","affiliation":[]}],"member":"297","published-online":{"date-parts":[[2010,5,31]]},"reference":[{"key":"2044_CR1","doi-asserted-by":"publisher","first-page":"341","DOI":"10.1089\/hum.1997.8.3-341","volume":"8","author":"LM Chen","year":"1997","unstructured":"Chen LM, Chao L, Chao J: Adenovirus-mediated delivery of human kallistatin gene reduces blood pressure of spontaneously hypertensive rats. 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