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The goal of this study was to provide a detailed evaluation of lung cancer tumor markers indicative of molecular abnormalities and to assess their diagnostic utility in non-small cell lung cancer (NSCLC) patients.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Methods<\/jats:title>\n            <jats:p>Quantitative real-time RT-PCR was used to determine <jats:italic>LunX, CK19, CEA, VEGF-C<\/jats:italic> and <jats:italic>hnRNP A2\/B1<\/jats:italic> mRNA levels in peripheral blood and pleural fluid from NSCLC patients, compared with those from patients with other epithelial cancer (esophagus cancer and breast cancer), benign lung disease (pneumonia and tuberculo pleurisy) and from healthy volunteers.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Results<\/jats:title>\n            <jats:p>In peripheral blood <jats:italic>LunX<\/jats:italic> mRNA was detectable in 75.0% (33\/44) of patients with NSCLC, but not in patients with other epithelial cancer (0\/28), benign lung disease (0\/10) or in healthy volunteers (0\/15). In contrast, all other genetic markers were detected in patients with either NSCLC, other epithelia cancer or benign lung disease, and in healthy volunteers. The expression level and positive rate of <jats:italic>LunX<\/jats:italic> mRNA in peripheral blood correlated with the pathologic stage of NSCLC (P &lt; 0.001 and P = 0.010 respectively). Furthermore, <jats:italic>LunX<\/jats:italic> mRNA was detected in 92.9% (13\/14) of malignant pleural fluid samples and was the only marker whose expression level was significantly different between malignant and benign pleural fluid (P &lt; 0.001). Additionally, expression of <jats:italic>LunX<\/jats:italic> mRNA in the peripheral blood of NSCLC patients decreased shortly after clinical treatment (P = 0.005).<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Conclusion<\/jats:title>\n            <jats:p>Of several commonly used genetic markers, <jats:italic>LunX<\/jats:italic> mRNA is the most specific gene marker for lung cancer and has potential diagnostic utility when measured in the peripheral blood and pleural fluid of NSCLC patients.<\/jats:p>\n          <\/jats:sec>","DOI":"10.1186\/1471-2407-8-156","type":"journal-article","created":{"date-parts":[[2008,5,31]],"date-time":"2008-05-31T06:13:20Z","timestamp":1212214400000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":33,"title":["Diagnostic utility of LunXmRNA in peripheral blood and pleural fluid in patients with primary non-small cell lung cancer"],"prefix":"10.1186","volume":"8","author":[{"given":"Min","family":"Cheng","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yongyan","family":"Chen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaoqing","family":"Yu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhigang","family":"Tian","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Haiming","family":"Wei","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2008,5,31]]},"reference":[{"issue":"4","key":"1094_CR1","doi-asserted-by":"publisher","first-page":"433","DOI":"10.1002\/1097-0215(200002)9999:9999<::AID-IJC1059>3.0.CO;2-B","volume":"91","author":"K Iwao","year":"2001","unstructured":"Iwao K, Watanabe T, Fujiwara Y, Takami K, Kodama K, Higashiyama M, Yokouchi H, Ozaki K, Monden M, Tanigami A: Isolation of a novel human lung-specific gene, LUNX, a potential molecular marker for detection of micrometastasis in non-small-cell lung cancer. 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