{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T09:49:00Z","timestamp":1791193740765,"version":"4.1.0"},"reference-count":22,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2009,2,16]],"date-time":"2009-02-16T00:00:00Z","timestamp":1234742400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/www.springer.com\/tdm"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["BMC Cancer"],"published-print":{"date-parts":[[2009,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n          <jats:sec>\n            <jats:title>Background<\/jats:title>\n            <jats:p>We previously found that r-hu-IFN\u03b3 exerts a potent anti-tumor effect on human nasopharyngeal carcinoma xenografts <jats:italic>in vivo<\/jats:italic>. Considering the fact that the clinical use of recombinant IFN\u03b3 is limited by its short half-life and systemic side effects, we developed a recombinant adenovirus, Ad-IFN\u03b3.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Methods<\/jats:title>\n            <jats:p>Dynamic distribution of the adenovirus vector and expression of IFN\u03b3 were evaluated by Q-PCR and ELISA after intratumoral administration of Ad-IFN\u03b3 into CNE-2 xenografts.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Results<\/jats:title>\n            <jats:p>Ad-IFN\u03b3 DNA was mainly enriched in tumors where the Ad-IFN\u03b3 DNA was injected (<jats:italic>P<\/jats:italic> &lt; 0.05, compared to blood or parenchymal organs), as well as in livers (<jats:italic>P<\/jats:italic> &lt; 0.05). Concentrations of Ad-IFN\u03b3 DNA in other organs and blood were very low. Intratumoral Ad-IFN\u03b3 DNA decreased sharply at high concentrations (9 \u00d7 10<jats:sup>5<\/jats:sup> copies\/\u03bcg tissue DNA), and slowly at lower concentrations (1.7\u20132.9 \u00d7 10<jats:sup>5<\/jats:sup> copies\/\u03bcg tissue DNA). IFN\u03b3 was detected in the tumors and parenchymal organs. The concentration of IFN\u03b3 was highest in the tumor (<jats:italic>P<\/jats:italic> &lt; 0.05), followed by the liver and kidney (<jats:italic>P<\/jats:italic> &lt; 0.05). High-level intratumoral expression of IFN\u03b3 was maintained for at least 7 days, rapidly peaking on day 3 after injection of Ad-IFN\u03b3 DNA.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Conclusion<\/jats:title>\n            <jats:p>An IFN\u03b3 gene delivered by an adenoviral vector achieved high and consistent intratumoral expression. Disseminated Ad-IFN\u03b3 DNA and the transgene product were mainly enriched in the liver.<\/jats:p>\n          <\/jats:sec>","DOI":"10.1186\/1471-2407-9-55","type":"journal-article","created":{"date-parts":[[2009,2,17]],"date-time":"2009-02-17T07:13:08Z","timestamp":1234854788000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Dynamic distribution and expression in vivo of the human interferon gamma gene delivered by adenoviral vector"],"prefix":"10.1186","volume":"9","author":[{"given":"Jiangxue","family":"Wu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xia","family":"Xiao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hongyun","family":"Jia","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiemin","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yinghui","family":"Zhu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peng","family":"Zhao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huanxin","family":"Lin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenlin","family":"Huang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2009,2,16]]},"reference":[{"issue":"1","key":"1389_CR1","doi-asserted-by":"publisher","first-page":"2","DOI":"10.1002\/jps.10007","volume":"91","author":"HM Younes","year":"2002","unstructured":"Younes HM, Amsden BG: Interferon-gamma therapy: evaluation of routes of administration and delivery systems. J Pharm Sci. 2002, 91 (1): 2-17. 10.1002\/jps.10007.","journal-title":"J Pharm Sci"},{"key":"1389_CR2","first-page":"1","volume":"116","author":"KR Pang","year":"2005","unstructured":"Pang KR, Wu JJ, Huang DB, Tyring SK, Baron S: Biological and clinical basis for molecular studies of interferons. Methods Mol Med. 2005, 116: 1-23.","journal-title":"Methods Mol Med"},{"key":"1389_CR3","doi-asserted-by":"publisher","first-page":"14567","DOI":"10.1073\/pnas.0404845101","volume":"101 Suppl 2","author":"M Liu","year":"2004","unstructured":"Liu M, Acres B, Balloul JM, Bizouarne N, Paul S, Slos P, et al: Gene-based vaccines and immunotherapeutics. Proc Natl Acad Sci U S A. 2004, 101 Suppl 2: 14567-14571. 10.1073\/pnas.0404845101.","journal-title":"Proc Natl Acad Sci U S A"},{"issue":"5","key":"1389_CR4","first-page":"463","volume":"5","author":"S Chada","year":"2003","unstructured":"Chada S, Ramesh R, Mhashilkar AM: Cytokine- and chemokine-based gene therapy for cancer. Curr Opin Mol Ther. 2003, 5 (5): 463-474.","journal-title":"Curr Opin Mol Ther"},{"issue":"6","key":"1389_CR5","doi-asserted-by":"publisher","first-page":"794","DOI":"10.1053\/j.seminoncol.2004.09.008","volume":"31","author":"AT Chan","year":"2004","unstructured":"Chan AT, Teo PM, Huang DP: Pathogenesis and treatment of nasopharyngeal carcinoma. Semin Oncol. 2004, 31 (6): 794-801. 10.1053\/j.seminoncol.2004.09.008.","journal-title":"Semin Oncol"},{"key":"1389_CR6","doi-asserted-by":"publisher","first-page":"4702","DOI":"10.1158\/1078-0432.CCR-06-0520","volume":"15","author":"JX Wu","year":"2006","unstructured":"Wu JX, Xiao X, Zhao P, Xue G, Zhu YH, Zhu XF, et al: Minicircle-IFN\u03b3 Induces Anti-proliferative and Anti-tumoral Effects in Human Nasopharyngeal Carcinoma. Clinical Cancer Res. 2006, 15: 4702-13. 10.1158\/1078-0432.CCR-06-0520.","journal-title":"Clinical Cancer Res"},{"issue":"9","key":"1389_CR7","doi-asserted-by":"publisher","first-page":"695","DOI":"10.1016\/j.lfs.2007.05.028","volume":"81","author":"P Zhao","year":"2007","unstructured":"Zhao P, Zhu Y, Wu J, Liu R, Zhu X, Xiao X, et al: Adenovirus-mediated delivery of human IFNgamma gene inhibits prostate cancer growth. Life Sci. 2007, 81 (9): 695-701. 10.1016\/j.lfs.2007.05.028.","journal-title":"Life Sci"},{"issue":"3","key":"1389_CR8","doi-asserted-by":"publisher","first-page":"207","DOI":"10.1007\/BF02789331","volume":"3","author":"FL Graham","year":"1995","unstructured":"Graham FL, Prevec L: Methods for construction of adenovirus vectors. Mol Biotechnol. 1995, 3 (3): 207-220.","journal-title":"Mol Biotechnol"},{"issue":"15","key":"1389_CR9","doi-asserted-by":"publisher","first-page":"2121","DOI":"10.1016\/S0959-8049(03)00367-8","volume":"39","author":"JP Spano","year":"2003","unstructured":"Spano JP, Busson P, Atlan D, Bourhis J, Pignon JP, Esteban C: Nasopharyngeal carcinomas: an update. Eur J Cancer. 2003, 39 (15): 2121-2135. 10.1016\/S0959-8049(03)00367-8.","journal-title":"Eur J Cancer"},{"issue":"33","key":"1389_CR10","doi-asserted-by":"publisher","first-page":"5108","DOI":"10.1038\/sj.onc.1206556","volume":"22","author":"LS Young","year":"2003","unstructured":"Young LS, Murray PG: Epstein-Barr virus and oncogenesis: from latent genes to tumours. Oncogene. 2003, 22 (33): 5108-5121. 10.1038\/sj.onc.1206556.","journal-title":"Oncogene"},{"key":"1389_CR11","first-page":"2133","volume":"23","author":"N Wakisaka","year":"2003","unstructured":"Wakisaka N, Pagano JS: Epstein-Barr virus induces invasion and metastasis factors. Anticancer Res. 2003, 23: 2133-2138.","journal-title":"Anticancer Res"},{"issue":"5","key":"1389_CR12","doi-asserted-by":"publisher","first-page":"352","DOI":"10.1038\/sj.cgt.7700319","volume":"8","author":"L Weinrib","year":"2001","unstructured":"Weinrib L, Li JH, Donovan J, Huang D, Liu FF: Cisplatin chemotherapy plus adenoviral p53 gene therapy in EBV-positive and -negative nasopharyngeal carcinoma. Cancer Gene Ther. 2001, 8 (5): 352-360. 10.1038\/sj.cgt.7700319.","journal-title":"Cancer Gene Ther"},{"issue":"3","key":"1389_CR13","doi-asserted-by":"publisher","first-page":"607","DOI":"10.1016\/S0360-3016(98)00432-5","volume":"43","author":"JH Li","year":"1999","unstructured":"Li JH, Lax SA, Kim J, Klamut H, Liu FF: The effects of combining ionizing radiation and adenoviral p53 therapy in nasopharyngeal carcinoma. Int J Radiat Oncol Biol Phys. 1999, 43 (3): 607-616.","journal-title":"Int J Radiat Oncol Biol Phys"},{"issue":"6","key":"1389_CR14","doi-asserted-by":"publisher","first-page":"964","DOI":"10.1016\/j.ymthe.2003.08.018","volume":"8","author":"JH Li","year":"2003","unstructured":"Li JH, Shi W, Chia M, Sanchez-Sweatman O, Siatskas C, Huang D, et al: Efficacy of targeted FasL in nasopharyngeal carcinoma. Mol Ther. 2003, 8 (6): 964-973. 10.1016\/j.ymthe.2003.08.018.","journal-title":"Mol Ther"},{"key":"1389_CR15","doi-asserted-by":"publisher","first-page":"64","DOI":"10.1038\/sj.cgt.7700011","volume":"6","author":"Y Lu","year":"1999","unstructured":"Lu Y, Carraher J, Zhang Y, Armstrong J, Lerner J, Rogers WP, et al: Delivery of adenoviral vectors to the prostate for gene therapy. Cancer Gene Ther. 1999, 6: 64-72. 10.1038\/sj.cgt.7700011.","journal-title":"Cancer Gene Ther"},{"issue":"1","key":"1389_CR16","doi-asserted-by":"publisher","first-page":"137","DOI":"10.2217\/14796694.2.1.137","volume":"2","author":"DT Rein","year":"2006","unstructured":"Rein DT, Breidenbach M, Curiel DT: Current developments in adenovirus-based cancer gene therapy. Future Oncol. 2006, 2 (1): 137-143. 10.2217\/14796694.2.1.137.","journal-title":"Future Oncol"},{"issue":"11","key":"1389_CR17","first-page":"3625","volume":"7","author":"F Lohr","year":"2001","unstructured":"Lohr F, Huang Q, Hu K, Dewhirst MW, Li CY: Systemic vector leakage and transgene expression by intratumorally injected recombinant adenovirus vectors. Clin Cancer Res. 2001, 7 (11): 3625-3628.","journal-title":"Clin Cancer Res"},{"issue":"3","key":"1389_CR18","doi-asserted-by":"publisher","first-page":"263","DOI":"10.1006\/mthe.2000.0037","volume":"1","author":"DL Paielli","year":"2000","unstructured":"Paielli DL, Wing MS, Rogulski KR, Gilbert JD, Kolozsvary A, Kim JH, et al: Evaluation of the biodistribution, persistence, toxicity, and potential of germ-line transmission of a replication-competent human adenovirus following intraprostatic administration in the mouse. Mol Ther. 2000, 1 (3): 263-274. 10.1006\/mthe.2000.0037.","journal-title":"Mol Ther"},{"issue":"11","key":"1389_CR19","first-page":"1233","volume":"2","author":"Y Wang","year":"2003","unstructured":"Wang Y, Hu JK, Krol A, Li YP, Li CY, Yuan F: Systemic dissemination of viral vectors during intratumoral injection. Mol Cancer Ther. 2003, 2 (11): 1233-1242.","journal-title":"Mol Cancer Ther"},{"issue":"8","key":"1389_CR20","doi-asserted-by":"publisher","first-page":"1414","DOI":"10.1038\/sj.bjc.6602494","volume":"92","author":"Y Wang","year":"2005","unstructured":"Wang Y, Yang Z, Liu S, Kon T, Krol A, Li CY, et al: Characterisation of systemic dissemination of nonreplicating adenoviral vectors from tumours in local gene delivery. Br J Cancer. 2005, 92 (8): 1414-1420. 10.1038\/sj.bjc.6602494.","journal-title":"Br J Cancer"},{"issue":"23","key":"1389_CR21","doi-asserted-by":"publisher","first-page":"11705","DOI":"10.1128\/JVI.01320-08","volume":"82","author":"Z Xu","year":"2008","unstructured":"Xu Z, Tian J, Smith JS, Byrnes AP: Clearance of adenovirus by Kupffer cells is mediated by scavenger receptors, natural antibodies and complement. J Virol. 2008, 82 (23): 11705-11713. 10.1128\/JVI.01320-08.","journal-title":"J Virol"},{"issue":"5","key":"1389_CR22","doi-asserted-by":"publisher","first-page":"547","DOI":"10.1089\/hum.2008.004","volume":"19","author":"JS Smith","year":"2008","unstructured":"Smith JS, Xu Z, Tian J, Stevenson SC, Byrnes AP: Interaction of systemically delivered adenovirus vectors with Kupffer cells in mouse liver. Hum Gene Ther. 2008, 19 (5): 547-54. 10.1089\/hum.2008.004.","journal-title":"Hum Gene Ther"}],"container-title":["BMC Cancer"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1186\/1471-2407-9-55.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/article\/10.1186\/1471-2407-9-55\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1186\/1471-2407-9-55","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1186\/1471-2407-9-55.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,9,1]],"date-time":"2021-09-01T10:38:48Z","timestamp":1630492728000},"score":1,"resource":{"primary":{"URL":"https:\/\/bmccancer.biomedcentral.com\/articles\/10.1186\/1471-2407-9-55"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2009,2,16]]},"references-count":22,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2009,12]]}},"alternative-id":["1389"],"URL":"https:\/\/doi.org\/10.1186\/1471-2407-9-55","relation":{},"ISSN":["1471-2407"],"issn-type":[{"value":"1471-2407","type":"electronic"}],"subject":[],"published":{"date-parts":[[2009,2,16]]},"assertion":[{"value":"10 June 2008","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"16 February 2009","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"16 February 2009","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}}],"article-number":"55"}}