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We report a novel, tissue-specific promoter that was identified and engineered from the native upstream regulatory region of the human gene <jats:italic>NDUFV1<\/jats:italic> containing an endogenous retroviral sequence.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Results<\/jats:title>\n            <jats:p>Among seven established human cell lines and five primary cultures, this modified <jats:italic>NDUFV1<\/jats:italic> upstream sequence (mNUS) was active only in human undifferentiated germ-derived cells (lines Tera-1 and EP2102), where it demonstrated high promoter activity (~twice greater than that of the SV40 early promoter, and comparable to the routinely used cytomegaloviral promoter). To investigate the potential applicability of the mNUS promoter for biotechnological needs, a construct carrying a recombinant cytosine deaminase (RCD) suicide gene under the control of mNUS was tested in cell lines of different tissue origin. High cytotoxic effect of RCD with a cell-death rate ~60% was observed only in germ-derived cells (Tera-1), whereas no effect was seen in a somatic, kidney-derived control cell line (HEK293). In further experiments, we tested mNUS-driven expression of a hyperactive <jats:italic>Sleeping Beauty<\/jats:italic> transposase (SB100X). The mNUS-SB100X construct mediated stable transgene insertions exclusively in germ-derived cells, thereby providing further evidence of tissue-specificity of the mNUS promoter.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Conclusions<\/jats:title>\n            <jats:p>We conclude that mNUS may be used as an efficient promoter for tissue-specific gene expression in human germ-derived cells in many applications. Our data also suggest that the 91 bp-long sequence located exactly upstream <jats:italic>NDUFV1<\/jats:italic> transcriptional start site plays a crucial role in the activity of this gene promoter <jats:italic>in vitro<\/jats:italic> in the majority of tested cell types (10\/12), and an important role - in the rest two cell lines.<\/jats:p>\n          <\/jats:sec>","DOI":"10.1186\/1472-6750-10-58","type":"journal-article","created":{"date-parts":[[2010,8,27]],"date-time":"2010-08-27T18:14:40Z","timestamp":1282932880000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["Novel strong tissue specific promoter for gene expression in human germ cells"],"prefix":"10.1186","volume":"10","author":[{"given":"Denis","family":"Kuzmin","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elena","family":"Gogvadze","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Roman","family":"Kholodenko","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dawid P","family":"Grzela","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maxim","family":"Mityaev","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tatyana","family":"Vinogradova","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eugene","family":"Kopantzev","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Galina","family":"Malakhova","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria","family":"Suntsova","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dmitry","family":"Sokov","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zolt\u00e1n","family":"Ivics","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anton","family":"Buzdin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2010,8,17]]},"reference":[{"issue":"7-8","key":"526_CR1","doi-asserted-by":"publisher","first-page":"542","DOI":"10.1016\/j.addr.2009.02.006","volume":"61","author":"Z Dong","year":"2009","unstructured":"Dong Z, Nor JE: Transcriptional targeting of tumor endothelial cells for gene therapy. 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