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The R. microplus Bm86 protective antigen has been produced by recombinant DNA technology and shown to protect cattle against tick infestations.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>In this study, the genes for Bm86 (<jats:italic>R. microplus<\/jats:italic>), Ba86 (<jats:italic>R. annulatus<\/jats:italic>) and Bd86 (<jats:italic>R. decoloratus<\/jats:italic>) were cloned and characterized from African or Asian tick strains and the recombinant proteins were secreted and purified from<jats:italic>P. pastoris<\/jats:italic>. The secretion of recombinant Bm86 ortholog proteins in<jats:italic>P. pastoris<\/jats:italic>allowed for a simple purification process rendering a final product with high recovery (35\u201342%) and purity (80\u201385%) and likely to result in a more reproducible conformation closely resembling the native protein. Rabbit immunization experiments with recombinant proteins showed immune cross-reactivity between Bm86 ortholog proteins.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>These experiments support the development and testing of vaccines containing recombinant Bm86, Ba86 and Bd86 secreted in<jats:italic>P. pastoris<\/jats:italic>for the control of tick infestations in Africa.<\/jats:p><\/jats:sec>","DOI":"10.1186\/1472-6750-8-14","type":"journal-article","created":{"date-parts":[[2008,2,14]],"date-time":"2008-02-14T19:13:28Z","timestamp":1203016408000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":38,"title":["Expression of recombinant Rhipicephalus (Boophilus) microplus, R. annulatus and R. decoloratus Bm86 orthologs as secreted proteins in Pichia pastoris"],"prefix":"10.1186","volume":"8","author":[{"given":"Mario","family":"Canales","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jos\u00e9 M P\u00e9rez","family":"de la Lastra","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Victoria","family":"Naranjo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ard M","family":"Nijhof","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michelle","family":"Hope","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Frans","family":"Jongejan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jos\u00e9","family":"de la Fuente","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2008,2,14]]},"reference":[{"key":"283_CR1","doi-asserted-by":"publisher","first-page":"S15","DOI":"10.1017\/S0031182004005207","volume":"129","author":"SC Barker","year":"2004","unstructured":"Barker SC, Murrell A: Systematics and evolution of ticks with a list of valid genus and species names. 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