{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,6,5]],"date-time":"2024-06-05T16:52:25Z","timestamp":1717606345757},"reference-count":9,"publisher":"Springer Science and Business Media LLC","issue":"1","content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["BMC Biotechnol"],"published-print":{"date-parts":[[2008,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n          <jats:sec>\n            <jats:title>Background<\/jats:title>\n            <jats:p>High purity of tumour samples is a necessity for accurate genetic and expression analysis and is usually achieved by positive selection in chronic lymphocytic leukaemia (CLL).<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Results<\/jats:title>\n            <jats:p>We adapted a bifunctional rosette-based antibody cocktail for negative selection of B-cells for isolating CLL cells from peripheral blood (PB). PB samples from CLL patients were split into aliquots. One aliquot of each sample was enriched by density gradient centrifugation (DGC), while the other aliquot of each sample was incubated with an antibody cocktail for B-cell enrichment prior to DGC (RS+DGC). The purity of CLL cells after DGC averaged 74.1% (range: 15.9 \u2013 97.4%). Using RS+DGC, the purity averaged 93.8% (range: 80.4 \u2013 99.4%) with 23 of 29 (79%) samples showing CLL purities above 90%. RNA extracted from enriched CLL cells was of appropriately high quality for microarray analysis.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Conclusion<\/jats:title>\n            <jats:p>This study confirms the use of a bifunctional rosette-based antibody cocktail as an effective method for the purification of CLL cells from peripheral blood.<\/jats:p>\n          <\/jats:sec>","DOI":"10.1186\/1472-6750-8-6","type":"journal-article","created":{"date-parts":[[2008,2,26]],"date-time":"2008-02-26T07:14:59Z","timestamp":1204010099000},"update-policy":"http:\/\/dx.doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Negative selection of chronic lymphocytic leukaemia cells using a bifunctional rosette-based antibody cocktail"],"prefix":"10.1186","volume":"8","author":[{"given":"Salim","family":"Essakali","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dennis","family":"Carney","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David","family":"Westerman","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peter","family":"Gambell","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"John F","family":"Seymour","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alexander","family":"Dobrovic","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2008,1,29]]},"reference":[{"key":"275_CR1","doi-asserted-by":"publisher","first-page":"1385","DOI":"10.1038\/sj.leu.2404274","volume":"20","author":"FJ Staal","year":"2006","unstructured":"Staal FJ, Cario G, Cazzaniga G, Haferlach T, Heuser M, Hofmann WK, Mills K, Schrappe M, Stanulla M, Wingen LU, van Dongen JJ, Schlegelberger B: Consensus guidelines for microarray gene expression analyses in leukemia from three European leukemia networks. Leukemia. 2006, 20: 1385-92. 10.1038\/sj.leu.2404274.","journal-title":"Leukemia"},{"key":"275_CR2","doi-asserted-by":"crossref","first-page":"618","DOI":"10.1038\/sj.leu.2403685","volume":"19","author":"D de Ridder","year":"2005","unstructured":"de Ridder D, van der Linden CE, Schonewille T, Dik WA, Reinders MJ, van Dongen JJ, Staal FJ: Purity for clarity: the need for purification of tumor cells in DNA microarray studies. Leukemia. 2005, 19: 618-27.","journal-title":"Leukemia"},{"key":"275_CR3","doi-asserted-by":"publisher","first-page":"3937","DOI":"10.1200\/JCO.2004.12.133","volume":"22","author":"C Haslinger","year":"2004","unstructured":"Haslinger C, Schweifer N, Stilgenbauer S, Dohner H, Lichter P, Kraut N, Stratowa C, Abseher R: Microarray gene expression profiling of B-cell chronic lymphocytic leukemia subgroups defined by genomic aberrations and VH mutation status. J Clin Oncol. 2004, 22: 3937-49. 10.1200\/JCO.2004.12.133.","journal-title":"J Clin Oncol"},{"key":"275_CR4","doi-asserted-by":"publisher","first-page":"681","DOI":"10.1182\/blood-2004-10-4073","volume":"106","author":"S Falt","year":"2005","unstructured":"Falt S, Merup M, Tobin G, Thunberg U, Gahrton G, Rosenquist R, Wennborg A: Distinctive gene expression pattern in VH3-21 utilizing B-cell chronic lymphocytic leukemia. Blood. 2005, 106: 681-9. 10.1182\/blood-2004-10-4073.","journal-title":"Blood"},{"key":"275_CR5","doi-asserted-by":"publisher","first-page":"291","DOI":"10.1182\/blood-2003-04-1161","volume":"103","author":"T Stankovic","year":"2004","unstructured":"Stankovic T, Hubank M, Cronin D, Stewart GS, Fletcher D, Bignell CR, Alvi AJ, Austen B, Weston VJ, Fegan C, Byrd PJ, Moss PA, Taylor AM: Microarray analysis reveals that TP53- and ATM-mutant B-CLLs share a defect in activating proapoptotic responses after DNA damage but are distinguished by major differences in activating prosurvival responses. Blood. 2004, 103: 291-300. 10.1182\/blood-2003-04-1161.","journal-title":"Blood"},{"key":"275_CR6","doi-asserted-by":"publisher","first-page":"1428","DOI":"10.1182\/blood-2003-09-3236","volume":"104","author":"A Rosenwald","year":"2004","unstructured":"Rosenwald A, Chuang EY, Davis RE, Wiestner A, Alizadeh AA, Arthur DC, Mitchell JB, Marti GE, Fowler DH, Wilson WH, Staudt LM: Fludarabine treatment of patients with chronic lymphocytic leukemia induces a p53-dependent gene expression response. Blood. 2004, 104: 1428-34. 10.1182\/blood-2003-09-3236.","journal-title":"Blood"},{"key":"275_CR7","doi-asserted-by":"publisher","first-page":"4944","DOI":"10.1182\/blood-2002-10-3306","volume":"101","author":"A Wiestner","year":"2003","unstructured":"Wiestner A, Rosenwald A, Barry TS, Wright G, Davis RE, Henrickson SE, Zhao H, Ibbotson RE, Orchard JA, Davis Z, Stetler-Stevenson M, Raffeld M, Arthur DC, Marti GE, Wilson WH, Hamblin TJ, Oscier DG, Staudt LM: ZAP-70 expression identifies a chronic lymphocytic leukemia subtype with unmutated immunoglobulin genes, inferior clinical outcome, and distinct gene expression profile. Blood. 2003, 101: 4944-51. 10.1182\/blood-2002-10-3306.","journal-title":"Blood"},{"key":"275_CR8","doi-asserted-by":"publisher","first-page":"1774","DOI":"10.1038\/sj.leu.2404363","volume":"20","author":"A Huttmann","year":"2006","unstructured":"Huttmann A, Klein-Hitpass L, Thomale J, Deenen R, Carpinteiro A, Nuckel H, Ebeling P, Fuhrer A, Edelmann J, Sellmann L, Duhrsen U, Durig J: Gene expression signatures separate B-cell chronic lymphocytic leukaemia prognostic subgroups defined by ZAP-70 and CD38 expression status. Leukemia. 2006, 20: 1774-82. 10.1038\/sj.leu.2404363.","journal-title":"Leukemia"},{"key":"275_CR9","first-page":"95","volume":"302","author":"CE Peters","year":"2005","unstructured":"Peters CE, Woodside SM, Eaves AC: Isolation of subsets of immune cells. Methods Mol Biol. 2005, 302: 95-116.","journal-title":"Methods Mol Biol"}],"container-title":["BMC Biotechnology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1186\/1472-6750-8-6.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,9,1]],"date-time":"2021-09-01T09:39:20Z","timestamp":1630489160000},"score":1,"resource":{"primary":{"URL":"https:\/\/bmcbiotechnol.biomedcentral.com\/articles\/10.1186\/1472-6750-8-6"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2008,1,29]]},"references-count":9,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2008,12]]}},"alternative-id":["275"],"URL":"https:\/\/doi.org\/10.1186\/1472-6750-8-6","relation":{},"ISSN":["1472-6750"],"issn-type":[{"value":"1472-6750","type":"electronic"}],"subject":[],"published":{"date-parts":[[2008,1,29]]},"assertion":[{"value":"17 October 2007","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"29 January 2008","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"29 January 2008","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}}],"article-number":"6"}}