{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,16]],"date-time":"2026-05-16T14:04:17Z","timestamp":1778940257301,"version":"3.51.4"},"reference-count":37,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2011,4,11]],"date-time":"2011-04-11T00:00:00Z","timestamp":1302480000000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/www.springer.com\/tdm"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["BMC Complement Altern Med"],"published-print":{"date-parts":[[2011,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n          <jats:sec>\n            <jats:title>Background<\/jats:title>\n            <jats:p>Tuberculosis, caused by <jats:italic>Mycobacterium tuberculosis<\/jats:italic> (MTB), is the most notified disease in the world. Development of resistance to first line drugs by MTB is a public health concern. As a result, there is the search for new and novel sources of antimycobacterial drugs for example from medicinal plants. In this study we determined the <jats:italic>in vitro<\/jats:italic> antimycobacterial activity of <jats:italic>n<\/jats:italic>-Hexane sub-fraction from <jats:italic>Bridelia micrantha<\/jats:italic> (Berth) against MTB H<jats:sub>37<\/jats:sub>Ra and a clinical isolate resistant to all five first-line antituberculosis drugs.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Methods<\/jats:title>\n            <jats:p>The antimycobacterial activity of the <jats:italic>n<\/jats:italic>-Hexane sub-fraction of ethyl acetate fractions from acetone extracts of <jats:italic>B. micrantha<\/jats:italic> barks was evaluated using the resazurin microplate assay against two MTB isolates. Bioassay-guided fractionation of the ethyl acetate fraction was performed using 100% <jats:italic>n<\/jats:italic>-Hexane and Chloroform\/Methanol (99:1) as solvents in order of increasing polarity by column chromatography and Resazurin microtiter plate assay for susceptibility tests.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Results<\/jats:title>\n            <jats:p>The <jats:italic>n<\/jats:italic>-Hexane fraction showed 20% inhibition of MTB H<jats:sub>37<\/jats:sub>Ra and almost 35% inhibition of an MTB isolate resistant to all first-line drugs at 10 \u03bcg\/mL. GC\/MS analysis of the fraction resulted in the identification of twenty-four constituents representing 60.5% of the fraction. Some of the 24 compounds detected included Benzene, 1.3-bis (3-phenoxyphenoxy (13.51%), 2-pinen-4-one (10.03%), N(b)-benzyl-14-(carboxymethyl) (6.35%) and the least detected compound was linalool (0.2%).<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Conclusions<\/jats:title>\n            <jats:p>The results show that the <jats:italic>n-<\/jats:italic> Hexane fraction of <jats:italic>B. micrantha<\/jats:italic> has antimycobacterial activity.<\/jats:p>\n          <\/jats:sec>","DOI":"10.1186\/1472-6882-11-28","type":"journal-article","created":{"date-parts":[[2011,4,13]],"date-time":"2011-04-13T06:15:37Z","timestamp":1302675337000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["Characterization of n-Hexane sub-fraction of Bridelia micrantha (Berth) and its antimycobacterium activity"],"prefix":"10.1186","volume":"11","author":[{"given":"Ezekiel","family":"Green","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lawrence C","family":"Obi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Amidou","family":"Samie","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pascal O","family":"Bessong","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Roland N","family":"Ndip","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2011,4,11]]},"reference":[{"key":"383_CR1","volume-title":"Fact sheets on tuberculosis","author":"WHO-World Health Organization","year":"2009","unstructured":"WHO-World Health Organization: Fact sheets on tuberculosis. 2009, [http:\/\/www.who.int\/tb\/en\/]"},{"key":"383_CR2","doi-asserted-by":"publisher","first-page":"1055","DOI":"10.1164\/ajrccm.163.5.2007122","volume":"162","author":"RJ O'Brien","year":"2001","unstructured":"O'Brien RJ, Nunn PP: The need for new drugs against tuberculosis: obstacles, opportunities, and next steps. 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