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The basic aim of this research was to investigate the antioxidant potential, total Phenolic and flavonoids contents and photochemical screening of the crude methanol extract and its derived various fractions <jats:italic>Dicliptera roxburghiana<\/jats:italic> of Acanthaceae family.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Methods<\/jats:title>\n            <jats:p>Crude methanol extract of aerial parts of <jats:italic>Dicliptera roxburghiana<\/jats:italic> (DRME) was partitioned in to <jats:italic>n<\/jats:italic>-hexane (DRHF), chloroform (DRCF), ethyl acetate (DREF), <jats:italic>n<\/jats:italic>-butanol (DRBF) and the remaining soluble portion as residual aqueous fraction (DRAF). We evaluated the antioxidant activities of the extract and various fractions through different analytical methods such as DPPH, superoxide anion, ABTS, H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub>, hydroxyl radical and phosphomolybdate radical inhibition. <jats:italic>In vitro<\/jats:italic> lipid peroxidation and reducing power of the plant was also analyzed. Total flavonoid and phenolic contents of the extract and all fractions were also quantified. Plant was also subjected for preliminary phytochemical screening to confirm the presence or absence of various constituents in the plant.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Results<\/jats:title>\n            <jats:p>Phytochemical screening confirmed the presence of flavonoids, phenolics, tannins, alkaloids, saponins, terpenoids and coumarines. Quantitative analysis revealed the maximum amount of total phenolic and flavonoid contents in DRME while lowest in DRHF. Methanol extract, DREF, DRCF and DRBF exhibited promising antioxidant potential for DPPH, ABTS, H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub>, phosphomolybdate, superoxide anion and hydroxyl radical scavenging capabilities, while these were not appreciable for DRHF and DRAF. All fractions except DRHF and DRAF possess strong reducing power ability and showed appreciable lipid peroxidation inhibition.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Conclusion<\/jats:title>\n            <jats:p>These research investigations revealed that <jats:italic>Dicliptera roxburghiana<\/jats:italic> is a potent source of natural antioxidants. Hence the plant can be used for management of different stress and anxiety related ailments.<\/jats:p>\n          <\/jats:sec>","DOI":"10.1186\/1472-6882-13-140","type":"journal-article","created":{"date-parts":[[2013,6,19]],"date-time":"2013-06-19T08:14:35Z","timestamp":1371629675000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":24,"title":["In vitro antioxidant potential of dicliptera roxburghiana"],"prefix":"10.1186","volume":"13","author":[{"given":"Bushra","family":"Ahmad","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Muhammad Rashid","family":"Khan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Naseer Ali","family":"Shah","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rahmat Ali","family":"Khan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2013,6,19]]},"reference":[{"key":"1415_CR1","doi-asserted-by":"publisher","first-page":"21","DOI":"10.1186\/1472-6882-10-21","volume":"10","author":"OA Aiyegoro","year":"2010","unstructured":"Aiyegoro OA, Okoh AI: Preliminary phytochemical screening and in vitro antioxidant activities of the aqueous extract of Helichrysum longifolium DC. 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