{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,6,12]],"date-time":"2025-06-12T07:51:35Z","timestamp":1749714695016,"version":"3.40.4"},"reference-count":10,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2013,2,14]],"date-time":"2013-02-14T00:00:00Z","timestamp":1360800000000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/www.springer.com\/tdm"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["BMC Complement Altern Med"],"published-print":{"date-parts":[[2013,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p><jats:italic>Prunus yedoensis<\/jats:italic>Matsum. is used as traditional medicine\u2014\u2018Yaeng-Pi\u2019 or \u2018Hua-Pi\u2019\u2014in Japan and Korea. However, no studies have examined the pharmacological activities of the<jats:italic>P. yedoensis<\/jats:italic>bark. Only the antioxidant and antiviral activities of<jats:italic>P. yedoensis<\/jats:italic>fruit and the anti-hyperglycaemic effect of<jats:italic>P. yedoensis<\/jats:italic>leaf have been investigated. While studying the antihypertensive effects of several medicinal plants, we found that a methanol extract of<jats:italic>P. yedoensis<\/jats:italic>bark (MEPY) had distinct vasorelaxant effects on rat aortic rings.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>The aortic rings were removed from Sprague\u2013Dawley rats and suspended in organ chambers containing 10\u00a0ml Krebs-Henseleit solution. The aortic rings were placed between 2 tungsten stirrups and connected to an isometric force transducer. Changes in tension were recorded via isometric transducers connected to a data acquisition system.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>MEPY relaxed the contraction induced by phenylephrine (PE) both in endothelium-intact and endothelium-denuded aortic rings concentration dependently. However, the vasorelaxant effects of MEPY on endothelium-denuded aortic rings were lower than endothelium-intact aortic rings. The vasorelaxant effects of MEPY on endothelium-intact aortic rings were reduced by pre-treatment with<jats:sc>l<\/jats:sc>-NAME, methylene blue, or ODQ. However, pre-treatment with indomethacin, atropine, glibenclamide, tetraethylammonium, or 4-aminopyridine had no affection. In addition, MEPY inhibited the contraction induced by extracellular Ca<jats:sup>2+<\/jats:sup>in endothelium-denuded rat thoracic aorta rings pre-contracted by PE (1\u00a0\u03bcM) or KCl (60\u00a0mM) in Ca<jats:sup>2+<\/jats:sup>-free solution.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Our results suggest that MEPY exerts its vasorelaxant effects via the activation of NO formation by means of<jats:sc>l<\/jats:sc>-Arg and NO-cGMP pathways and via the blockage of extracellular Ca<jats:sup>2+<\/jats:sup>channels.<\/jats:p><\/jats:sec>","DOI":"10.1186\/1472-6882-13-31","type":"journal-article","created":{"date-parts":[[2013,2,14]],"date-time":"2013-02-14T17:14:03Z","timestamp":1360862043000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":27,"title":["Vasorelaxant effect of Prunus yedoensis bark"],"prefix":"10.1186","volume":"13","author":[{"given":"Kyungjin","family":"Lee","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Inhye","family":"Ham","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gabsik","family":"Yang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mihwa","family":"Lee","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Youngmin","family":"Bu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hocheol","family":"Kim","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ho-Young","family":"Choi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2013,2,14]]},"reference":[{"key":"1280_CR1","doi-asserted-by":"publisher","first-page":"185","DOI":"10.1266\/jjg.70.185","volume":"70","author":"H Innan","year":"1995","unstructured":"Innan H, Terauchi R, Miyashita NT, Tsunewaki K: DNA fingerprinting study on the intraspecific variation and the origin of Prunus yedoensis (Someiyoshino). 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