{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,12]],"date-time":"2026-05-12T19:22:22Z","timestamp":1778613742868,"version":"3.51.4"},"reference-count":27,"publisher":"Springer Science and Business Media LLC","issue":"1","content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Microb Cell Fact"],"published-print":{"date-parts":[[2013,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n          <jats:sec>\n            <jats:title>Backgroud<\/jats:title>\n            <jats:p>Daptomycin is an important antibiotic against infections caused by drug-resistant pathogens. Its production critically depends on the addition of decanoic acid during fermentation. Unfortunately, decanoic acid (&gt;2.5\u00a0mM) is toxic to daptomycin producer, <jats:italic>Streptomyces roseosporus<\/jats:italic>.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Results<\/jats:title>\n            <jats:p>To understand the mechanism underlying decanoic tolerance or toxicity, the responses of <jats:italic>S. roseosporus<\/jats:italic> was determined by a combination of phospholipid fatty acid analysis, reactive oxygen species (ROS) measurement and RNA sequencing. Assays using fluorescent dyes indicated a sharp increase in reactive oxygen species during decanoic acid stress; fatty acid analysis revealed a marked increase in the composition of branched-chain fatty acids by approximately 10%, with a corresponding decrease in straight-chain fatty acids; functional analysis indicated decanoic acid stress has components common to other stress response, including perturbation of respiratory functions (<jats:italic>nuo<\/jats:italic> and <jats:italic>cyd<\/jats:italic> operons), oxidative stress, and heat shock. Interestingly, our transcriptomic analysis revealed that genes coding for components of proteasome and related to treholase synthesis were up-regulated in the decanoic acid \u2013treated cells.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Conclusion<\/jats:title>\n            <jats:p>These findings represent an important first step in understanding mechanism of decanoic acid toxicity and provide a basis for engineering microbial tolerance.<\/jats:p>\n          <\/jats:sec>","DOI":"10.1186\/1475-2859-12-19","type":"journal-article","created":{"date-parts":[[2013,2,22]],"date-time":"2013-02-22T00:32:28Z","timestamp":1361493148000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":26,"title":["Transcriptional analysis of the effect of exogenous decanoic acid stress on Streptomyces roseosporus"],"prefix":"10.1186","volume":"12","author":[{"given":"Guojian","family":"Liao","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qing","family":"Liu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jianping","family":"Xie","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2013,2,21]]},"reference":[{"key":"814_CR1","doi-asserted-by":"publisher","first-page":"144","DOI":"10.1016\/j.cbpa.2009.02.031","volume":"13","author":"RH Baltz","year":"2009","unstructured":"Baltz RH: Daptomycin: mechanisms of action and resistance, and biosynthetic engineering. 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