{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,4]],"date-time":"2025-11-04T15:55:45Z","timestamp":1762271745073},"reference-count":38,"publisher":"Springer Science and Business Media LLC","issue":"1","content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Microb Cell Fact"],"published-print":{"date-parts":[[2013,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n          <jats:sec>\n            <jats:title>Background<\/jats:title>\n            <jats:p>Ribosome assembly cofactor RimP is one of the auxiliary proteins required for maturation of the 30S subunit in <jats:italic>Escherichia coli<\/jats:italic>. Although RimP in protein synthesis is important, its role in secondary metabolites biosynthesis has not been reported so far. Considering the close relationship between protein synthesis and the production of secondary metabolites, the function of ribosome assembly cofactor RimP on antibiotics production was studied in <jats:italic>Streptomyces coelicolor<\/jats:italic> and <jats:italic>Streptomyces venezuelae<\/jats:italic>.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Results<\/jats:title>\n            <jats:p>In this study, the <jats:italic>rimP<\/jats:italic> homologue <jats:italic>rimP-SC<\/jats:italic> was identified and cloned from <jats:italic>Streptomyces coelicolor<\/jats:italic>. Disruption of <jats:italic>rimP-SC<\/jats:italic> led to enhanced production of actinorhodin and calcium-dependent antibiotics by promoting the transcription of <jats:italic>act<\/jats:italic> II-ORF4 and <jats:italic>cdaR<\/jats:italic>. Further experiments demonstrated that MetK was one of the reasons for the increment of antibiotics production. In addition, <jats:italic>rimP-SC<\/jats:italic> disruption mutant could be used as a host to produce more peptidyl nucleoside antibiotics (polyoxin or nikkomycin) than the wild-type strain. Likewise, disruption of <jats:italic>rimP-SV<\/jats:italic> of <jats:italic>Streptomyces venezuelae<\/jats:italic> also significantly stimulated jadomycin production, suggesting that enhanced antibiotics production might be widespread in many other <jats:italic>Streptomyces<\/jats:italic> species.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Conclusion<\/jats:title>\n            <jats:p>These results established an important relationship between ribosome assembly cofactor and secondary metabolites biosynthesis and provided an approach for yield improvement of secondary metabolites in <jats:italic>Streptomyces.<\/jats:italic>\n            <\/jats:p>\n          <\/jats:sec>","DOI":"10.1186\/1475-2859-12-65","type":"journal-article","created":{"date-parts":[[2013,7,2]],"date-time":"2013-07-02T06:15:07Z","timestamp":1372745707000},"update-policy":"http:\/\/dx.doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Disruption of rimP-SC, encoding a ribosome assembly cofactor, markedly enhances the production of several antibiotics in Streptomyces coelicolor"],"prefix":"10.1186","volume":"12","author":[{"given":"Yuanyuan","family":"Pan","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cheng","family":"Lu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hailing","family":"Dong","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lingjun","family":"Yu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gang","family":"Liu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Huarong","family":"Tan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2013,7,2]]},"reference":[{"key":"860_CR1","volume-title":"Physiology of the Bacterial Cell. A Molecular Approach","author":"F Neidhardt","year":"1990","unstructured":"Neidhardt F, Ingraham J, Schaechter M: Physiology of the Bacterial Cell. A Molecular Approach. 1990, Sunderland, MA: Sinauer Associates, Inc, Publishers"},{"key":"860_CR2","doi-asserted-by":"publisher","first-page":"742","DOI":"10.1016\/j.jmb.2008.12.076","volume":"386","author":"S Nord","year":"2009","unstructured":"Nord S, Bylund GO, Lovgren JM, Wikstrom PM: The RimP protein is important for maturation of the 30S ribosomal subunit. J Mol Biol. 2009, 386: 742-753. 10.1016\/j.jmb.2008.12.076","journal-title":"J Mol Biol"},{"key":"860_CR3","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1016\/j.jmb.2010.02.036","volume":"398","author":"AE Bunner","year":"2010","unstructured":"Bunner AE, Nord S, Wikstrom PM, Williamson JR: The effect of ribosome assembly cofactors on in vitro 30S subunit reconstitution. J Mol Biol. 2010, 398: 1-7. 10.1016\/j.jmb.2010.02.036","journal-title":"J Mol Biol"},{"key":"860_CR4","doi-asserted-by":"publisher","first-page":"501","DOI":"10.1146\/annurev-biochem-062608-160432","volume":"80","author":"Z Shajani","year":"2011","unstructured":"Shajani Z, Sykes MT, Williamson JR: Assembly of bacterial ribosomes. Annu Rev Biochem. 2011, 80: 501-526. 10.1146\/annurev-biochem-062608-160432","journal-title":"Annu Rev Biochem"},{"issue":"Pt 9","key":"860_CR5","doi-asserted-by":"publisher","first-page":"2183","DOI":"10.1099\/00221287-145-9-2183","volume":"145","author":"DA Hopwood","year":"1999","unstructured":"Hopwood DA: Forty years of genetics with Streptomyces: from in vivo through in vitro to in silico. Microbiology. 1999, 145 (Pt 9): 2183-2202.","journal-title":"Microbiology"},{"key":"860_CR6","volume-title":"Practical Streptomyces Genetics","author":"T Kieser","year":"2000","unstructured":"Kieser T, Bibb MJ, Buttner MJ, Chater KF, Hopwood DA: Practical Streptomyces Genetics. 2000, Norwich, England: John Innes Foundation"},{"key":"860_CR7","doi-asserted-by":"publisher","first-page":"462","DOI":"10.1038\/309462a0","volume":"309","author":"F Malpartida","year":"1984","unstructured":"Malpartida F, Hopwood DA: Molecular cloning of the whole biosynthetic pathway of a Streptomyces antibiotic and its expression in a heterologous host. Nature. 1984, 309: 462-464. 10.1038\/309462a0","journal-title":"Nature"},{"key":"860_CR8","doi-asserted-by":"publisher","first-page":"817","DOI":"10.1016\/S1074-5521(01)00054-0","volume":"8","author":"AM Cerdeno","year":"2001","unstructured":"Cerdeno AM, Bibb MJ, Challis GL: Analysis of the prodiginine biosynthesis gene cluster of Streptomyces coelicolor A3(2): new mechanisms for chain initiation and termination in modular multienzymes. Chem Biol. 2001, 8: 817-829. 10.1016\/S1074-5521(01)00054-0","journal-title":"Chem Biol"},{"key":"860_CR9","doi-asserted-by":"publisher","first-page":"1175","DOI":"10.1016\/S1074-5521(02)00252-1","volume":"9","author":"Z Hojati","year":"2002","unstructured":"Hojati Z, Milne C, Harvey B, Gordon L, Borg M, Flett F, Wilkinson B, Sidebottom PJ, Rudd BA, Hayes MA, et al: Structure, biosynthetic origin, and engineered biosynthesis of calcium-dependent antibiotics from Streptomyces coelicolor. Chem Biol. 2002, 9: 1175-1187. 10.1016\/S1074-5521(02)00252-1","journal-title":"Chem Biol"},{"key":"860_CR10","doi-asserted-by":"publisher","first-page":"2166","DOI":"10.1002\/cbic.200500243","volume":"6","author":"C Corre","year":"2005","unstructured":"Corre C, Challis GL: Evidence for the unusual condensation of a diketide with a pentulose in the methylenomycin biosynthetic pathway of Streptomyces coelicolor A3(2). ChemBioChem. 2005, 6: 2166-2170. 10.1002\/cbic.200500243","journal-title":"ChemBioChem"},{"key":"860_CR11","doi-asserted-by":"publisher","first-page":"1555","DOI":"10.1099\/mic.0.2008\/018523-0","volume":"154","author":"GL Challis","year":"2008","unstructured":"Challis GL: Mining microbial genomes for new natural products and biosynthetic pathways. Microbiology. 2008, 154: 1555-1569. 10.1099\/mic.0.2008\/018523-0","journal-title":"Microbiology"},{"key":"860_CR12","doi-asserted-by":"publisher","first-page":"1362","DOI":"10.1039\/b817069j","volume":"26","author":"M Nett","year":"2009","unstructured":"Nett M, Ikeda H, Moore BS: Genomic basis for natural product biosynthetic diversity in the actinomycetes. Nat Prod Rep. 2009, 26: 1362-1384. 10.1039\/b817069j","journal-title":"Nat Prod Rep"},{"key":"860_CR13","doi-asserted-by":"publisher","first-page":"1424","DOI":"10.7164\/antibiotics.53.1424","volume":"53","author":"Y Okamoto-Hosoya","year":"2000","unstructured":"Okamoto-Hosoya Y, Sato TA, Ochi K: Resistance to paromomycin is conferred by rpsL mutations, accompanied by an enhanced antibiotic production in Streptomyces coelicolor A3(2). J Antibiot. 2000, 53: 1424-1427. 10.7164\/antibiotics.53.1424","journal-title":"J Antibiot"},{"key":"860_CR14","doi-asserted-by":"publisher","first-page":"155","DOI":"10.1016\/S0065-2164(04)56005-7","volume":"56","author":"K Ochi","year":"2004","unstructured":"Ochi K, Okamoto S, Tozawa Y, Inaoka T, Hosaka T, Xu J, Kurosawa K: Ribosome engineering and secondary metabolite production. Adv Appl Microbiol. 2004, 56: 155-184.","journal-title":"Adv Appl Microbiol"},{"key":"860_CR15","doi-asserted-by":"publisher","first-page":"601","DOI":"10.1128\/JB.185.2.601-609.2003","volume":"185","author":"S Okamoto","year":"2003","unstructured":"Okamoto S, Lezhava A, Hosaka T, Okamoto-Hosoya Y, Ochi K: Enhanced expression of S-adenosylmethionine synthetase causes overproduction of actinorhodin in Streptomyces coelicolor A3(2). J Bacteriol. 2003, 185: 601-609. 10.1128\/JB.185.2.601-609.2003","journal-title":"J Bacteriol"},{"key":"860_CR16","doi-asserted-by":"publisher","first-page":"1065","DOI":"10.1016\/j.jmb.2007.10.003","volume":"374","author":"D Sharma","year":"2007","unstructured":"Sharma D, Cukras AR, Rogers EJ, Southworth DR, Green R: Mutational analysis of S12 protein and implications for the accuracy of decoding by the ribosome. J Mol Biol. 2007, 374: 1065-1076. 10.1016\/j.jmb.2007.10.003","journal-title":"J Mol Biol"},{"key":"860_CR17","doi-asserted-by":"publisher","first-page":"721","DOI":"10.1016\/S0092-8674(02)01086-3","volume":"111","author":"JM Ogle","year":"2002","unstructured":"Ogle JM, Murphy FV, Tarry MJ, Ramakrishnan V: Selection of tRNA by the ribosome requires a transition from an open to a closed form. Cell. 2002, 111: 721-732. 10.1016\/S0092-8674(02)01086-3","journal-title":"Cell"},{"key":"860_CR18","doi-asserted-by":"publisher","first-page":"897","DOI":"10.1126\/science.1060612","volume":"292","author":"JM Ogle","year":"2001","unstructured":"Ogle JM, Brodersen DE, Clemons WM, Tarry MJ, Carter AP, Ramakrishnan V: Recognition of cognate transfer RNA by the 30S ribosomal subunit. Science. 2001, 292: 897-902. 10.1126\/science.1060612","journal-title":"Science"},{"key":"860_CR19","doi-asserted-by":"publisher","first-page":"1019","DOI":"10.1128\/AAC.00388-08","volume":"53","author":"G Wang","year":"2009","unstructured":"Wang G, Inaoka T, Okamoto S, Ochi K: A novel insertion mutation in Streptomyces coelicolor ribosomal S12 protein results in paromomycin resistance and antibiotic overproduction. Antimicrob Agents Chemother. 2009, 53: 1019-1026. 10.1128\/AAC.00388-08","journal-title":"Antimicrob Agents Chemother"},{"key":"860_CR20","doi-asserted-by":"publisher","first-page":"532","DOI":"10.7164\/antibiotics.50.532","volume":"50","author":"A Hesketh","year":"1997","unstructured":"Hesketh A, Ochi K: A novel method for improving Streptomyces coelicolor A3(2) for production of actinorhodin by introduction of rpsL (encoding ribosomal protein S12) mutations conferring resistance to streptomycin. J Antibiot. 1997, 50: 532-535. 10.7164\/antibiotics.50.532","journal-title":"J Antibiot"},{"key":"860_CR21","doi-asserted-by":"publisher","first-page":"883","DOI":"10.1111\/j.1365-2958.2006.05285.x","volume":"61","author":"T Hosaka","year":"2006","unstructured":"Hosaka T, Xu J, Ochi K: Increased expression of ribosome recycling factor is responsible for the enhanced protein synthesis during the late growth phase in an antibiotic-overproducing Streptomyces coelicolor ribosomal rpsL mutant. Mol Microbiol. 2006, 61: 883-897. 10.1111\/j.1365-2958.2006.05285.x","journal-title":"Mol Microbiol"},{"key":"860_CR22","doi-asserted-by":"publisher","first-page":"3299","DOI":"10.1099\/mic.0.26490-0","volume":"149","author":"Y Okamoto-Hosoya","year":"2003","unstructured":"Okamoto-Hosoya Y, Hosaka T, Ochi K: An aberrant protein synthesis activity is linked with antibiotic overproduction in rpsL mutants of Streptomyces coelicolor A3(2). Microbiology. 2003, 149: 3299-3309. 10.1099\/mic.0.26490-0","journal-title":"Microbiology"},{"key":"860_CR23","doi-asserted-by":"publisher","first-page":"6412","DOI":"10.1128\/AEM.69.11.6412-6417.2003","volume":"69","author":"N Tamehiro","year":"2003","unstructured":"Tamehiro N, Hosaka T, Xu J, Hu H, Otake N, Ochi K: Innovative approach for improvement of an antibiotic-overproducing industrial strain of Streptomyces albus. Appl Environ Microbiol. 2003, 69: 6412-6417. 10.1128\/AEM.69.11.6412-6417.2003","journal-title":"Appl Environ Microbiol"},{"key":"860_CR24","doi-asserted-by":"publisher","first-page":"2384","DOI":"10.1099\/mic.0.039834-0","volume":"156","author":"G Wang","year":"2010","unstructured":"Wang G, Tanaka Y, Ochi K: The G243D mutation (afsB mutation) in the principal sigma factor sigmaHrdB alters intracellular ppGpp level and antibiotic production in Streptomyces coelicolor A3(2). Microbiology. 2010, 156: 2384-2392. 10.1099\/mic.0.039834-0","journal-title":"Microbiology"},{"key":"860_CR25","doi-asserted-by":"publisher","first-page":"2834","DOI":"10.1128\/AEM.02800-07","volume":"74","author":"G Wang","year":"2008","unstructured":"Wang G, Hosaka T, Ochi K: Dramatic activation of antibiotic production in Streptomyces coelicolor by cumulative drug resistance mutations. Appl Environ Microbiol. 2008, 74: 2834-2840. 10.1128\/AEM.02800-07","journal-title":"Appl Environ Microbiol"},{"key":"860_CR26","doi-asserted-by":"publisher","first-page":"3701","DOI":"10.1038\/emboj.2010.229","volume":"29","author":"I Wohlgemuth","year":"2010","unstructured":"Wohlgemuth I, Pohl C, Rodnina MV: Optimization of speed and accuracy of decoding in translation. EMBO J. 2010, 29: 3701-3709. 10.1038\/emboj.2010.229","journal-title":"EMBO J"},{"key":"860_CR27","doi-asserted-by":"publisher","first-page":"175","DOI":"10.1007\/s00284-001-0115-4","volume":"45","author":"H Zeng","year":"2002","unstructured":"Zeng H, Tan H, Li J: Cloning and function of sanQ: a gene involved in nikkomycin biosynthesis of Streptomyces ansochromogenes. Curr Microbiol. 2002, 45: 175-179. 10.1007\/s00284-001-0115-4","journal-title":"Curr Microbiol"},{"key":"860_CR28","doi-asserted-by":"publisher","first-page":"165","DOI":"10.1016\/S0378-1119(01)00723-5","volume":"279","author":"K Yang","year":"2001","unstructured":"Yang K, Han L, He J, Wang L, Vining LC: A repressor-response regulator gene pair controlling jadomycin B production in Streptomyces venezuelae ISP5230. Gene. 2001, 279: 165-173. 10.1016\/S0378-1119(01)00723-5","journal-title":"Gene"},{"key":"860_CR29","doi-asserted-by":"publisher","first-page":"553","DOI":"10.1046\/j.1365-2958.2001.02240.x","volume":"39","author":"TB Anderson","year":"2001","unstructured":"Anderson TB, Brian P, Champness WC: Genetic and transcriptional analysis of absA, an antibiotic gene cluster-linked two-component system that regulates multiple antibiotics in Streptomyces coelicolor. Mol Microbiol. 2001, 39: 553-566. 10.1046\/j.1365-2958.2001.02240.x","journal-title":"Mol Microbiol"},{"key":"860_CR30","doi-asserted-by":"publisher","first-page":"8617","DOI":"10.1073\/pnas.0900592106","volume":"106","author":"L Wang","year":"2009","unstructured":"Wang L, Tian X, Wang J, Yang H, Fan K, Xu G, Yang K, Tan H: Autoregulation of antibiotic biosynthesis by binding of the end product to an atypical response regulator. Proc Natl Acad Sci U S A. 2009, 106: 8617-8622. 10.1073\/pnas.0900592106","journal-title":"Proc Natl Acad Sci U S A"},{"key":"860_CR31","doi-asserted-by":"publisher","first-page":"120","DOI":"10.1007\/BF01584109","volume":"13","author":"JL Doull","year":"1994","unstructured":"Doull JL, Singh AK, Hoare M, Ayer SW: Conditions for the production of jadomycin B by Streptomyces venezuelae ISP5230: effects of heat shock, ethanol treatment and phage infection. J Ind Microbiol. 1994, 13: 120-125. 10.1007\/BF01584109","journal-title":"J Ind Microbiol"},{"key":"860_CR32","doi-asserted-by":"publisher","first-page":"6640","DOI":"10.1073\/pnas.120163297","volume":"97","author":"KA Datsenko","year":"2000","unstructured":"Datsenko KA, Wanner BL: One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products. Proc Natl Acad Sci U S A. 2000, 97: 6640-6645. 10.1073\/pnas.120163297","journal-title":"Proc Natl Acad Sci U S A"},{"key":"860_CR33","doi-asserted-by":"crossref","first-page":"204","DOI":"10.1128\/JB.181.1.204-211.1999","volume":"181","author":"MS Paget","year":"1999","unstructured":"Paget MS, Chamberlin L, Atrih A, Foster SJ, Buttner MJ: Evidence that the extracytoplasmic function sigma factor sigmaE is required for normal cell wall structure in Streptomyces coelicolor A3(2). J Bacteriol. 1999, 181: 204-211.","journal-title":"J Bacteriol"},{"key":"860_CR34","doi-asserted-by":"publisher","first-page":"6","DOI":"10.1186\/1475-2859-9-6","volume":"9","author":"G Liao","year":"2010","unstructured":"Liao G, Li J, Li L, Yang H, Tian Y, Tan H: Cloning, reassembling and integration of the entire nikkomycin biosynthetic gene cluster into Streptomyces ansochromogenes lead to an improved nikkomycin production. Microb Cell Fact. 2010, 9: 6- 10.1186\/1475-2859-9-6","journal-title":"Microb Cell Fact"},{"key":"860_CR35","doi-asserted-by":"publisher","first-page":"135","DOI":"10.1186\/1475-2859-11-135","volume":"11","author":"J Li","year":"2012","unstructured":"Li J, Li L, Feng C, Chen Y, Tan H: Novel polyoxins generated by heterologously expressing polyoxin biosynthetic gene cluster in the sanN inactivated mutant of Streptomyces ansochromogenes. Microb Cell Fact. 2012, 11: 135- 10.1186\/1475-2859-11-135","journal-title":"Microb Cell Fact"},{"key":"860_CR36","doi-asserted-by":"publisher","first-page":"1855","DOI":"10.1111\/j.1365-2958.2005.04512.x","volume":"55","author":"G Liu","year":"2005","unstructured":"Liu G, Tian Y, Yang H, Tan H: A pathway-specific transcriptional regulatory gene for nikkomycin biosynthesis in Streptomyces ansochromogenes that also influences colony development. Mol Microbiol. 2005, 55: 1855-1866. 10.1111\/j.1365-2958.2005.04512.x","journal-title":"Mol Microbiol"},{"key":"860_CR37","doi-asserted-by":"publisher","first-page":"710","DOI":"10.1111\/j.1365-2958.2009.06681.x","volume":"72","author":"Y Pan","year":"2009","unstructured":"Pan Y, Liu G, Yang H, Tian Y, Tan H: The pleiotropic regulator AdpA-L directly controls the pathway-specific activator of nikkomycin biosynthesis in Streptomyces ansochromogenes. Mol Microbiol. 2009, 72: 710-723. 10.1111\/j.1365-2958.2009.06681.x","journal-title":"Mol Microbiol"},{"key":"860_CR38","doi-asserted-by":"publisher","first-page":"164","DOI":"10.1186\/1471-2180-11-164","volume":"11","author":"Y Pan","year":"2011","unstructured":"Pan Y, Wang L, He X, Tian Y, Liu G, Tan H: SabR enhances nikkomycin production via regulating the transcriptional level of sanG, a pathway-specific regulatory gene in Streptomyces ansochromogenes. BMC Microbiol. 2011, 11: 164- 10.1186\/1471-2180-11-164","journal-title":"BMC Microbiol"}],"container-title":["Microbial Cell Factories"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1186\/1475-2859-12-65.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,9,2]],"date-time":"2021-09-02T00:42:34Z","timestamp":1630543354000},"score":1,"resource":{"primary":{"URL":"https:\/\/microbialcellfactories.biomedcentral.com\/articles\/10.1186\/1475-2859-12-65"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,7,2]]},"references-count":38,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2013,12]]}},"alternative-id":["860"],"URL":"https:\/\/doi.org\/10.1186\/1475-2859-12-65","relation":{},"ISSN":["1475-2859"],"issn-type":[{"value":"1475-2859","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,7,2]]},"assertion":[{"value":"28 March 2013","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"26 June 2013","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"2 July 2013","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}}],"article-number":"65"}}