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The mosquito species that act as vectors of human plasmodia in South East Asia, Africa and South America show different susceptibilities to infection by rodent <jats:italic>Plasmodium<\/jats:italic> species. <jats:italic>P. berghei<\/jats:italic> and <jats:italic>P. yoelii<\/jats:italic> infect both <jats:italic>Anopheles gambiae<\/jats:italic> and <jats:italic>Anopheles stephensi<\/jats:italic>, which are found mainly in Africa and Asia, respectively. However, it was reported that <jats:italic>P. yoelii<\/jats:italic> can infect the South American mosquito, <jats:italic>Anopheles albimanus<\/jats:italic>, while <jats:italic>P. berghei<\/jats:italic> cannot.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Methods<\/jats:title>\n            <jats:p>\n              <jats:italic>P. berghei<\/jats:italic> lines that express the green fluorescent protein were used to screen for mosquitoes that are susceptible to infection by <jats:italic>P. berghei<\/jats:italic>. Live mosquitoes were examined and screened for the presence of a fluorescent signal in the abdomen. Infected mosquitoes were then examined by time-lapse microscopy to reveal the dynamic behaviour of sporozoites in haemolymph and extracted salivary glands.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Results<\/jats:title>\n            <jats:p>A single fluorescent oocyst can be detected in live mosquitoes and <jats:italic>P. berghei<\/jats:italic> can infect <jats:italic>A. albimanus<\/jats:italic>. As in other mosquitoes, <jats:italic>P. berghei<\/jats:italic> sporozoites can float through the haemolymph and invade <jats:italic>A. albimanus<\/jats:italic> salivary glands and they are infectious in mice after subcutaneous injection.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Conclusion<\/jats:title>\n            <jats:p>Fluorescent <jats:italic>Plasmodium<\/jats:italic> parasites can be used to rapidly screen susceptible mosquitoes. These results open the way to develop a laboratory model in countries where importation of <jats:italic>A. gambiae<\/jats:italic> and <jats:italic>A. stephensi<\/jats:italic> is not allowed.<\/jats:p>\n          <\/jats:sec>","DOI":"10.1186\/1475-2875-5-23","type":"journal-article","created":{"date-parts":[[2006,4,6]],"date-time":"2006-04-06T12:50:36Z","timestamp":1144327836000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":24,"title":["Using green fluorescent malaria parasites to screen for permissive vector mosquitoes"],"prefix":"10.1186","volume":"5","author":[{"given":"Friedrich","family":"Frischknecht","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Beatrice","family":"Martin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Isabelle","family":"Thiery","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Catherine","family":"Bourgouin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Robert","family":"Menard","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2006,3,28]]},"reference":[{"issue":"7","key":"201_CR1","doi-asserted-by":"publisher","first-page":"679","DOI":"10.1016\/j.ibmb.2004.03.026","volume":"34","author":"I Siden-Kiamos","year":"2004","unstructured":"Siden-Kiamos I, Louis C: Interactions between malaria parasites and their mosquito hosts in the midgut. 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