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Conjugation experiments revealed the presence of a large conjugative plasmid (pLM07\/20) with an exclusive FrepB replicon type (IncF\/FIB). PCR analysis and sequencing confirmed the presence of the<jats:italic>bla<\/jats:italic><jats:sub>CTX-M-14<\/jats:sub>gene in the pLM07\/20 from<jats:italic>C. freundii<\/jats:italic>.LM07\/10. Although this is the first report of CTX-M-14 in Venezuela, we alert the medical community that future increase of these \u03b2-lactamases in our city could be due to dissemination of plasmids into bacterial populations.<\/jats:p>","DOI":"10.1186\/1476-0711-10-22","type":"journal-article","created":{"date-parts":[[2011,6,1]],"date-time":"2011-06-01T06:19:26Z","timestamp":1306909166000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["CTX-M-14 \u03b2-lactamase-producing Citrobacter freundii isolated in Venezuela"],"prefix":"10.1186","volume":"10","author":[{"given":"Beatriz","family":"Mill\u00e1n","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"B\u00e1rbara","family":"Ghiglione","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tulia","family":"D\u00edaz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gabriel","family":"Gutkind","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mar\u00eda","family":"Araque","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2011,5,31]]},"reference":[{"issue":"3","key":"225_CR1","doi-asserted-by":"publisher","first-page":"313","DOI":"10.2165\/11533040-000000000-00000","volume":"70","author":"JDD Pitout","year":"2010","unstructured":"Pitout JDD: Infections with extended-spectrum \u03b2-lactamase-producing Enterobacteriaceae. 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