{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T13:36:58Z","timestamp":1790775418738,"version":"4.1.0"},"reference-count":30,"publisher":"Springer Science and Business Media LLC","issue":"1","content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Ann Clin Microbiol Antimicrob"],"published-print":{"date-parts":[[2010,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n          <jats:sec>\n            <jats:title>Background<\/jats:title>\n            <jats:p>Chronic lung infection with the bacterium <jats:italic>Pseudomonas aeruginosa<\/jats:italic> is one of the hallmarks of cystic fibrosis (CF) and is associated with worsening lung function, increased hospitalisation and reduced life expectancy. A virulent clonal strain of <jats:italic>P. aeruginosa<\/jats:italic> (Australian epidemic strain I; AES-I) has been found to be widespread in CF patients in eastern Australia.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Methods<\/jats:title>\n            <jats:p>Suppression subtractive hybridization (SSH) was employed to identify genetic sequences that are present in the AES-I strain but absent from the sequenced reference strain PAO1. We used PCR to evaluate the distribution of several of the AES-I loci amongst a collection of 188 <jats:italic>P. aeruginosa<\/jats:italic> isolates which was comprised of 35 AES-I isolates (as determined by PFGE), 78 non-AES-I CF isolates including other epidemic CF strains as well as 69 <jats:italic>P. aeruginosa<\/jats:italic> isolates from other clinical and environmental sources.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Results<\/jats:title>\n            <jats:p>We have identified a unique AES-I genetic locus that is present in all 35 AES-I isolates tested and not present in any of the other 153 <jats:italic>P. aeruginosa<\/jats:italic> strains examined. We have used this unique AES-I locus to develop a diagnostic PCR and a real-time PCR assay to detect the presence of <jats:italic>P. aeruginosa<\/jats:italic> and AES-I in patient sputum samples.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Conclusions<\/jats:title>\n            <jats:p>We have developed diagnostic PCR assays that are 100% sensitive and 100% specific for the <jats:italic>P. aeruginosa<\/jats:italic> strain AES-I. We have also shown that Whatman FTA<jats:sup>\u00ae<\/jats:sup> Elute cards may be used with PCR-based assays to rapidly detect the presence of <jats:italic>P. aeruginosa<\/jats:italic> strains in CF sputum.<\/jats:p>\n          <\/jats:sec>","DOI":"10.1186\/1476-0711-9-18","type":"journal-article","created":{"date-parts":[[2010,7,17]],"date-time":"2010-07-17T06:13:47Z","timestamp":1279347227000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["A diagnostic PCR assay for the detection of an Australian epidemic strain of Pseudomonas aeruginosa"],"prefix":"10.1186","volume":"9","author":[{"given":"Heidi L","family":"Williams","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lynne","family":"Turnbull","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Susan J","family":"Thomas","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna","family":"Murphy","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tim","family":"Stinear","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David S","family":"Armstrong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cynthia B","family":"Whitchurch","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2010,7,16]]},"reference":[{"key":"190_CR1","doi-asserted-by":"publisher","first-page":"2266","DOI":"10.1128\/JCM.41.5.2266-2267.2003","volume":"41","author":"D Armstrong","year":"2003","unstructured":"Armstrong D, Bell S, Robinson M, Bye P, Rose B, Harbour C, Lee C, Service H, Nissen M, Syrmis M, et al.: Evidence for spread of a clonal strain of Pseudomonas aeruginosa among cystic fibrosis clinics. 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