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This malignant V\u03b45 T cell clone disappeared after chemotherapy, but the clone was detected again when disease relapsed post allogeneic hematopoietic stem cell transplantation (allo-HSCT) at 100\u00a0weeks. Using PCR and GeneScan analyses, the distribution and clonality of the TCR V\u03b3 and V\u03b4 subfamilies were examined before and after allo-HSCT in the patient. A reactive T cell clone with a V\u03b44D\u03b43J\u03b41 rearrangement was identified in all samples taken at different time points (i.e., 4, 8, 68, 100 and 108\u00a0weeks after allo-HSCT). The expression of this V\u03b44+ T cell clone was higher in the patient during complete remission (CR) post allo-HSCT and at disease relapse.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>This study established a sensitive methodology to detect T cell subclones, which may be used to monitor minimal residual disease and immune reconstitution.<\/jats:p><\/jats:sec>","DOI":"10.1186\/1476-4598-12-73","type":"journal-article","created":{"date-parts":[[2013,7,13]],"date-time":"2013-07-13T00:13:59Z","timestamp":1373674439000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["The evolution of malignant and reactive \u03b3\u03b4\u2009+\u2009T cell clones in a relapse T-ALL case after allogeneic stem cell transplantation"],"prefix":"10.1186","volume":"12","author":[{"given":"Shaohua","family":"Chen","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xin","family":"Huang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haitao","family":"Zheng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Suxia","family":"Geng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiuli","family":"Wu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lijian","family":"Yang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianyu","family":"Weng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xin","family":"Du","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yangqiu","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2013,7,12]]},"reference":[{"key":"1142_CR1","doi-asserted-by":"publisher","first-page":"303","DOI":"10.1111\/j.1365-2141.2011.08957.x","volume":"15","author":"MD Kraszeska","year":"2012","unstructured":"Kraszeska MD, Dawidowska M, Szczepa\u0144ski T, Witt M: T-cell acute lymphoblastic leukemia: recent molecular biology findings. 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