{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,31]],"date-time":"2025-10-31T13:52:37Z","timestamp":1761918757196},"reference-count":12,"publisher":"Springer Science and Business Media LLC","issue":"1","content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Mol Cancer"],"published-print":{"date-parts":[[2005,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n          <jats:sec>\n            <jats:title>Background<\/jats:title>\n            <jats:p>\n              <jats:italic>Myc<\/jats:italic>-induced lymphoblastic B-cell lymphoma (LBL) in iMyc<jats:sup>E\u03bc<\/jats:sup> mice may provide a model system for the study of the mechanism by which human <jats:italic>MYC<\/jats:italic> facilitates the initiation and progression of B cell and plasma cell neoplasms in human beings. We have recently shown that gene-targeted iMyc<jats:sup>E\u03bc<\/jats:sup> mice that carry a His<jats:sub>6<\/jats:sub>-tagged mouse <jats:italic>Myc<\/jats:italic> cDNA, <jats:italic>Myc<\/jats:italic>\n              <jats:sup>His<\/jats:sup>, just 5' of the immunoglobulin heavy-chain enhancer, E\u03bc, are prone to B cell and plasma cell tumors. The predominant tumor (~50%) that arose in the iMyc<jats:sup>E\u03bc<\/jats:sup> mice on the mixed genetic background of segregating C57BL\/6 and 129\/SvJ alleles was LBL. The purpose of this study was to establish and characterize a cell line, designated iMyc<jats:sup>E\u03bc<\/jats:sup>-1, for the in-depth evaluation of LBL <jats:italic>in vitro<\/jats:italic>.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Methods<\/jats:title>\n            <jats:p>The morphological features and the surface marker expression profile of the iMyc<jats:sup>E\u03bc<\/jats:sup>-1 cells were evaluated using cytological methods and FACS, respectively. The cytogenetic make-up of the iMyc<jats:sup>E\u03bc<\/jats:sup>-1 cells was assessed by spectral karyotyping (SKY). The expression of the inserted <jats:italic>Myc<\/jats:italic>\n              <jats:sup>His<\/jats:sup> gene was determined using RT-PCR and qPCR. Clonotypic immunoglobulin gene arrangements were detected by Southern blotting. The global gene expression program of the iMyc<jats:sup>E\u03bc<\/jats:sup>-1 cells and the expression of 768 \"pathway\" genes were determined with the help of the Mouse Lymphochip<jats:sup>\u00a9<\/jats:sup> and Superarray<jats:sup>\u00a9<\/jats:sup> cDNA micro- and macroarrays, respectively. Array results were verified, in part, by RT-PCR and qPCR.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Results<\/jats:title>\n            <jats:p>Consistent with their derivation from LBL, the iMyc<jats:sup>E\u03bc<\/jats:sup>-1 cells were found to be neoplastic IgM<jats:sup>high<\/jats:sup>IgD<jats:sup>low<\/jats:sup> lymphoblasts that expressed typical B-cell surface markers including CD40, CD54 (ICAM-1), CD80 (B7-1) and CD86 (B7-2). The iMyc<jats:sup>E\u03bc<\/jats:sup>-1 cells harbored a reciprocal T(9;11) and three non-reciprocal chromosomal translocations, over-expressed <jats:italic>Myc<\/jats:italic>\n              <jats:sup>His<\/jats:sup> at the expense of normal <jats:italic>Myc<\/jats:italic>, and exhibited gene expression changes on Mouse Lymphochip<jats:sup>\u00a9<\/jats:sup> microarrays that were consistent with <jats:italic>Myc<\/jats:italic>\n              <jats:sup>His<\/jats:sup>-driven B-cell neoplasia. Upon comparison to normal B cells using eight different Superarray<jats:sup>\u00a9<\/jats:sup> cDNA macroarrays, the iMyc<jats:sup>E\u03bc<\/jats:sup>-1 cells showed the highest number of changes on the NF\u03baB array.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Conclusion<\/jats:title>\n            <jats:p>The iMyc<jats:sup>E\u03bc<\/jats:sup>-1 cells may provide a uniquely useful model system to study the growth and survival requirements of <jats:italic>Myc<\/jats:italic>-driven mouse LBL <jats:italic>in vitro<\/jats:italic>.<\/jats:p>\n          <\/jats:sec>","DOI":"10.1186\/1476-4598-4-40","type":"journal-article","created":{"date-parts":[[2005,11,10]],"date-time":"2005-11-10T20:35:01Z","timestamp":1131654901000},"update-policy":"http:\/\/dx.doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Molecular and cytological features of the mouse B-cell lymphoma line iMycE\u03bc-1"],"prefix":"10.1186","volume":"4","author":[{"given":"Seong","family":"Su Han","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Arthur L","family":"Shaffer","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Liangping","family":"Peng","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Seung Tae","family":"Chung","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jae Hwan","family":"Lim","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sungho","family":"Maeng","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joong","family":"Su Kim","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nicole","family":"McNeil","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thomas","family":"Ried","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Louis M","family":"Staudt","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Siegfried","family":"Janz","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2005,11,9]]},"reference":[{"key":"129_CR1","doi-asserted-by":"publisher","first-page":"1306","DOI":"10.1158\/0008-5472.CAN-04-0268","volume":"65","author":"SS Park","year":"2005","unstructured":"Park SS, Kim JS, Tessarollo L, Owens JD, Peng L, Han SS, Tae Chung S, Torrey TA, Cheung WC, Polakiewicz RD, McNeil N, Ried T, Mushinski JF, Morse HC, Janz S: Insertion of c-Myc into Igh induces B-cell and plasma-cell neoplasms in mice. 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