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The present study was designed to assess the protective effects of methanolic extract of<jats:italic>Carissa opaca<\/jats:italic>leaves (MLC) on carbon tetrachloride (CCl<jats:sub>4<\/jats:sub>)-induced reproductive stress in male rats and bioactive constituents responsible for the activity.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>CCl<jats:sub>4<\/jats:sub>was induced in 42 male rats for eight weeks and checked the protective efficacy of methanolic extract of<jats:italic>Carissa opaca<\/jats:italic>leaves at various hormonal imbalances, alteration of antioxidant enzymes, DNA fragmentation levels and lipid peroxidation caused testicular fibrosis in testis while High performance Liquid Chromatography (HPLC) was used for detection of bioactive components.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>HPLC characterization revealed the presence of isoquercitin , hyperoside , vitexin , myricetin and kaempherol. CCl<jats:sub>4<\/jats:sub>caused significant alteration in the secretion of reproductive hormones. Activity of antioxidant enzymes viz; catalase, superoxide dimutase and phase II metabolizing enzymes including glutathione peroxidase, glutathione reductase and reduced glutathione was decreased while DNA fragmentation, hydrogen per oxide contents and thiobarbituric acid reactive substances (TBARS) were increased with CCl<jats:sub>4<\/jats:sub>treatment. Co-administration of 100 mg\/kg and 200 mg\/kg b.w. MLC effectively ameliorated the alterations in the biochemical markers; hormonal and molecular levels.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Protective effects of methanolic extract of<jats:italic>Carissa opaca<\/jats:italic>against CCl<jats:sub>4\u2212<\/jats:sub>induced antioxidant and hormonal dysfunction which might be due to bioactive compound present in extract.<\/jats:p><\/jats:sec>","DOI":"10.1186\/1476-511x-12-90","type":"journal-article","created":{"date-parts":[[2013,6,20]],"date-time":"2013-06-20T16:15:10Z","timestamp":1371744910000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":32,"title":["Effect of Carissa opaca leaves extract on lipid peroxidation, antioxidant activity and reproductive hormones in male rats"],"prefix":"10.1186","volume":"12","author":[{"given":"Sumaira","family":"Sahreen","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Muhammad Rashid","family":"Khan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rahmat Ali","family":"Khan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Naseer Ali","family":"Shah","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2013,6,20]]},"reference":[{"issue":"3","key":"911_CR1","doi-asserted-by":"publisher","first-page":"177","DOI":"10.1016\/j.lfs.2007.05.005","volume":"81","author":"SK Das","year":"2007","unstructured":"Das SK, Vasudevan DM: Alcohol induced oxidative stress. 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