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The predominant fatty acids were found to be C<jats:sub>18:1<\/jats:sub>\u03c97c (32.8%), C<jats:sub>16:1<\/jats:sub>\u03c97c (27.3%) and C<jats:sub>16:0<\/jats:sub> (23.1%). Phylogenetic analysis of the 16S rRNA gene revealed that this strain belonging to the genus <jats:italic>Pseudomonas<\/jats:italic>. Strain C2 was found to be closely related to <jats:italic>Pseudomonas luteola<\/jats:italic> with more than 99% of similarity. Amylase optimization extraction was carried out using Box Behnken Design (BBD). Its maximal activity was found when the pH and temperature ranged from 5.5 to 6.5 and from 33 to 37\u00b0C, respectively. 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