{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T23:05:20Z","timestamp":1791327920674,"version":"4.3.1"},"reference-count":32,"publisher":"Springer Science and Business Media LLC","issue":"1","content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["J Transl Med"],"published-print":{"date-parts":[[2010,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n          <jats:p>Human stem cells from adult sources have been shown to contribute to the regeneration of muscle, liver, heart, and vasculature. The mechanisms by which this is accomplished are, however, still not well understood. We tested the engraftment and regenerative potential of human umbilical cord blood-derived ALDH<jats:sup>hi<\/jats:sup>Lin<jats:sup>-<\/jats:sup>, and ALDH<jats:sup>lo<\/jats:sup>Lin<jats:sup>-<\/jats:sup> cells following transplantation to NOD\/SCID or NOD\/SCID \u03b22m null mice with experimentally induced acute myocardial infarction. We used combined nanoparticle labeling and whole organ fluorescent imaging to detect human cells in multiple organs 48 hours post transplantation. Engraftment and regenerative effects of cell treatment were assessed four weeks post transplantation. We found that ALDH<jats:sup>hi<\/jats:sup>Lin<jats:sup>-<\/jats:sup> stem cells specifically located to the site of injury 48 hours post transplantation and engrafted the infarcted heart at higher frequencies than ALDH<jats:sup>lo<\/jats:sup>Lin<jats:sup>-<\/jats:sup> committed progenitor cells four weeks post transplantation. We found no donor derived cardiomyocytes and few endothelial cells of donor origin. Cell treatment was not associated with any detectable functional improvement at the four week endpoint. There was, however, a significant increase in vascular density in the central infarct zone of ALDH<jats:sup>hi<\/jats:sup>Lin<jats:sup>-<\/jats:sup> cell-treated mice, as compared to PBS and ALDH<jats:sup>lo<\/jats:sup>Lin<jats:sup>-<\/jats:sup> cell-treated mice.<\/jats:p>\n          <jats:p>\n            <jats:bold>Conclusions<\/jats:bold>\n          <\/jats:p>\n          <jats:p>Our data indicate that adult human stem cells do not become a significant part of the regenerating tissue, but rapidly home to and persist only temporarily at the site of hypoxic injury to exert trophic effects on tissue repair thereby enhancing vascular recovery.<\/jats:p>","DOI":"10.1186\/1479-5876-8-24","type":"journal-article","created":{"date-parts":[[2010,3,30]],"date-time":"2010-03-30T06:15:29Z","timestamp":1269929729000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":39,"title":["Human cord blood progenitors with high aldehyde dehydrogenase activity improve vascular density in a model of acute myocardial infarction"],"prefix":"10.1186","volume":"8","author":[{"given":"Claus S","family":"Sondergaard","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David A","family":"Hess","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dustin J","family":"Maxwell","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carla","family":"Weinheimer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ivana","family":"Rosov\u00e1","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael H","family":"Creer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David","family":"Piwnica-Worms","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Attila","family":"Kovacs","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lene","family":"Pedersen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jan A","family":"Nolta","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2010,3,9]]},"reference":[{"issue":"4","key":"446_CR1","doi-asserted-by":"publisher","first-page":"487","DOI":"10.1634\/stemcells.22-4-487","volume":"22","author":"JE Grove","year":"2004","unstructured":"Grove JE, Bruscia E, Krause DS: Plasticity of bone marrow-derived stem cells. Stem Cells. 2004, 22 (4): 487-500. 10.1634\/stemcells.22-4-487.","journal-title":"Stem Cells"},{"issue":"1","key":"446_CR2","doi-asserted-by":"publisher","first-page":"65","DOI":"10.1385\/SCR:1:1:065","volume":"1","author":"DS Vieyra","year":"2005","unstructured":"Vieyra DS, Jackson KA, Goodell MA: Plasticity and tissue regenerative potential of bone marrow-derived cells. Stem Cell Rev. 2005, 1 (1): 65-69. 10.1385\/SCR:1:1:065.","journal-title":"Stem Cell Rev"},{"issue":"10","key":"446_CR3","doi-asserted-by":"publisher","first-page":"3828","DOI":"10.1073\/pnas.86.10.3828","volume":"86","author":"HE Broxmeyer","year":"1989","unstructured":"Broxmeyer HE, Douglas GW, Hangoc G, Cooper S, Bard J, English D, Arny M, Thomas L, Boyse EA: Human umbilical cord blood as a potential source of transplantable hematopoietic stem\/progenitor cells. Proc Natl Acad Sci USA. 1989, 86 (10): 3828-3832. 10.1073\/pnas.86.10.3828.","journal-title":"Proc Natl Acad Sci USA"},{"issue":"5","key":"446_CR4","doi-asserted-by":"publisher","first-page":"1669","DOI":"10.1182\/blood-2003-05-1670","volume":"103","author":"OK Lee","year":"2004","unstructured":"Lee OK, Kuo TK, Chen WM, Lee KD, Hsieh SL, Chen TH: Isolation of multipotent mesenchymal stem cells from umbilical cord blood. Blood. 2004, 103 (5): 1669-1675. 10.1182\/blood-2003-05-1670.","journal-title":"Blood"},{"issue":"11","key":"446_CR5","doi-asserted-by":"publisher","first-page":"1527","DOI":"10.1172\/JCI8296","volume":"105","author":"T Murohara","year":"2000","unstructured":"Murohara T, Ikeda H, Duan J, Shintani S, Sasaki K, Eguchi H, Onitsuka I, Matsui K, Imaizumi T: Transplanted cord blood-derived endothelial precursor cells augment postnatal neovascularization. J Clin Invest. 2000, 105 (11): 1527-1536. 10.1172\/JCI8296.","journal-title":"J Clin Invest"},{"issue":"3","key":"446_CR6","doi-asserted-by":"publisher","first-page":"259","DOI":"10.1080\/14653240701218516","volume":"9","author":"T Gentry","year":"2007","unstructured":"Gentry T, Foster S, Winstead L, Deibert E, Fiordalisi M, Balber A: Simultaneous isolation of human BM hematopoietic, endothelial and mesenchymal progenitor cells by flow sorting based on aldehyde dehydrogenase activity: implications for cell therapy. Cytotherapy. 2007, 9 (3): 259-274. 10.1080\/14653240701218516.","journal-title":"Cytotherapy"},{"issue":"1","key":"446_CR7","doi-asserted-by":"publisher","first-page":"99","DOI":"10.1046\/j.1365-2141.2003.04357.x","volume":"122","author":"P Fallon","year":"2003","unstructured":"Fallon P, Gentry T, Balber AE, Boulware D, Janssen WE, Smilee R, Storms RW, Smith C: Mobilized peripheral blood SSCloALDHbr cells have the phenotypic and functional properties of primitive haematopoietic cells and their number correlates with engraftment following autologous transplantation. Br J Haematol. 2003, 122 (1): 99-108. 10.1046\/j.1365-2141.2003.04357.x.","journal-title":"Br J Haematol"},{"issue":"6","key":"446_CR8","doi-asserted-by":"publisher","first-page":"1648","DOI":"10.1182\/blood-2004-02-0448","volume":"104","author":"DA Hess","year":"2004","unstructured":"Hess DA, Meyerrose TE, Wirthlin L, Craft TP, Herrbrich PE, Creer MH, Nolta JA: Functional characterization of highly purified human hematopoietic repopulating cells isolated according to aldehyde dehydrogenase activity. Blood. 2004, 104 (6): 1648-1655. 10.1182\/blood-2004-02-0448.","journal-title":"Blood"},{"issue":"5","key":"446_CR9","doi-asserted-by":"publisher","first-page":"2162","DOI":"10.1182\/blood-2005-06-2284","volume":"107","author":"DA Hess","year":"2006","unstructured":"Hess DA, Wirthlin L, Craft TP, Herrbrich PE, Hohm SA, Lahey R, Eades WC, Creer MH, Nolta JA: Selection based on CD133 and high aldehyde dehydrogenase activity isolates long-term reconstituting human hematopoietic stem cells. Blood. 2006, 107 (5): 2162-2169. 10.1182\/blood-2005-06-2284.","journal-title":"Blood"},{"issue":"16","key":"446_CR10","doi-asserted-by":"publisher","first-page":"9118","DOI":"10.1073\/pnas.96.16.9118","volume":"96","author":"RW Storms","year":"1999","unstructured":"Storms RW, Trujillo AP, Springer JB, Shah L, Colvin OM, Ludeman SM, Smith C: Isolation of primitive human hematopoietic progenitors on the basis of aldehyde dehydrogenase activity. Proc Natl Acad Sci USA. 1999, 96 (16): 9118-9123. 10.1073\/pnas.96.16.9118.","journal-title":"Proc Natl Acad Sci USA"},{"issue":"3","key":"446_CR11","doi-asserted-by":"publisher","first-page":"611","DOI":"10.1634\/stemcells.2007-0429","volume":"26","author":"DA Hess","year":"2008","unstructured":"Hess DA, Craft TP, Wirthlin L, Hohm S, Zhou P, Eades WC, Creer MH, Sands MS, Nolta JA: Widespread nonhematopoietic tissue distribution by transplanted human progenitor cells with high aldehyde dehydrogenase activity. Stem Cells. 2008, 26 (3): 611-620. 10.1634\/stemcells.2007-0429.","journal-title":"Stem Cells"},{"issue":"6","key":"446_CR12","doi-asserted-by":"crossref","first-page":"1262","DOI":"10.2144\/03356ss06","volume":"35","author":"TE Meyerrose","year":"2003","unstructured":"Meyerrose TE, Herrbrich P, Hess DA, Nolta JA: Immune-deficient mouse models for analysis of human stem cells. Biotechniques. 2003, 35 (6): 1262-1272.","journal-title":"Biotechniques"},{"issue":"10","key":"446_CR13","doi-asserted-by":"publisher","first-page":"2551","DOI":"10.1038\/nprot.2007.371","volume":"2","author":"LW van Laake","year":"2007","unstructured":"van Laake LW, Passier R, Monshouwer-Kloots J, Nederhoff MG, Oostwaard DWV, Field LJ, van Echteld CJ, Doevendans PA, Mummery CL: Monitoring of cell therapy and assessment of cardiac function using magnetic resonance imaging in a mouse model of myocardial infarction. Nat Protoc. 2007, 2 (10): 2551-2567. 10.1038\/nprot.2007.371.","journal-title":"Nat Protoc"},{"issue":"10","key":"446_CR14","doi-asserted-by":"crossref","first-page":"3102","DOI":"10.1182\/blood.V95.10.3102","volume":"95","author":"O Kollet","year":"2000","unstructured":"Kollet O, Peled A, Byk T, Ben-Hur H, Greiner D, Shultz L, Lapidot T: beta2 microglobulin-deficient (B2 m(null)) NOD\/SCID mice are excellent recipients for studying human stem cell function. Blood. 2000, 95 (10): 3102-3105.","journal-title":"Blood"},{"issue":"6","key":"446_CR15","doi-asserted-by":"publisher","first-page":"392","DOI":"10.1097\/01.moh.0000145672.42503.70","volume":"11","author":"J Bonde","year":"2004","unstructured":"Bonde J, Hess DA, Nolta JA: Recent advances in hematopoietic stem cell biology. Curr Opin Hematol. 2004, 11 (6): 392-398. 10.1097\/01.moh.0000145672.42503.70.","journal-title":"Curr Opin Hematol"},{"issue":"4","key":"446_CR16","first-page":"127","volume":"28","author":"V Koo","year":"2006","unstructured":"Koo V, Hamilton PW, Williamson K: Non-invasive in vivo imaging in small animal research. Cell Oncol. 2006, 28 (4): 127-139.","journal-title":"Cell Oncol"},{"issue":"2","key":"446_CR17","doi-asserted-by":"publisher","first-page":"517","DOI":"10.1634\/stemcells.2007-0016","volume":"26","author":"DJ Maxwell","year":"2008","unstructured":"Maxwell DJ, Bonde J, Hess DA, Hohm SA, Lahey R, Zhou P, Creer MH, Piwnica-Worms D, Nolta JA: Fluorophore-conjugated iron oxide nanoparticle labeling and analysis of engrafting human hematopoietic stem cells. Stem Cells. 2008, 26 (2): 517-524. 10.1634\/stemcells.2007-0016.","journal-title":"Stem Cells"},{"issue":"4","key":"446_CR18","doi-asserted-by":"publisher","first-page":"1455","DOI":"10.1128\/MCB.01369-06","volume":"27","author":"JM Lau","year":"2007","unstructured":"Lau JM, Jin X, Ren J, Avery J, DeBosch BJ, Treskov I, Lupu TS, Kovacs A, Weinheimer C, Muslin AJ: The 14-3-3tau phosphoserine-binding protein is required for cardiomyocyte survival. Molecular and cellular biology. 2007, 27 (4): 1455-1466. 10.1128\/MCB.01369-06.","journal-title":"Molecular and cellular biology"},{"issue":"5","key":"446_CR19","doi-asserted-by":"publisher","first-page":"567","DOI":"10.1172\/JCI6713","volume":"104","author":"JH Rogers","year":"1999","unstructured":"Rogers JH, Tamirisa P, Kovacs A, Weinheimer C, Courtois M, Blumer KJ, Kelly DP, Muslin AJ: RGS4 causes increased mortality and reduced cardiac hypertrophy in response to pressure overload. J Clin Invest. 1999, 104 (5): 567-576. 10.1172\/JCI6713.","journal-title":"J Clin Invest"},{"issue":"6","key":"446_CR20","doi-asserted-by":"publisher","first-page":"601","DOI":"10.1067\/mje.2002.117560","volume":"15","author":"S Kanno","year":"2002","unstructured":"Kanno S, Lerner DL, Schuessler RB, Betsuyaku T, Yamada KA, Saffitz JE, Kovacs A: Echocardiographic evaluation of ventricular remodeling in a mouse model of myocardial infarction. J Am Soc Echocardiogr. 2002, 15 (6): 601-609. 10.1067\/mje.2002.117560.","journal-title":"J Am Soc Echocardiogr"},{"issue":"2","key":"446_CR21","doi-asserted-by":"crossref","first-page":"393","DOI":"10.1111\/j.2517-6161.1994.tb01988.x","volume":"56","author":"AS Hadi","year":"1994","unstructured":"Hadi AS: A Modification of a Method for the Detection of Outliers in Multivariate Samples. Journal of the Royal Statistical Society Series B (Methodological). 1994, 56 (2): 393-","journal-title":"Journal of the Royal Statistical Society Series B (Methodological)"},{"issue":"5","key":"446_CR22","doi-asserted-by":"publisher","first-page":"223","DOI":"10.1007\/s002770050506","volume":"78","author":"Y Fukuchi","year":"1999","unstructured":"Fukuchi Y, Miyakawa Y, Kizaki M, Umezawa A, Shimamura K, Kobayashi K, Kuramochi T, Hata J, Ikeda Y, Tamaoki N: Human acute myeloblastic leukemia-ascites model using the human GM-CSF- and IL-3-releasing transgenic SCID mice. Annals of hematology. 1999, 78 (5): 223-231. 10.1007\/s002770050506.","journal-title":"Annals of hematology"},{"issue":"6","key":"446_CR23","doi-asserted-by":"publisher","first-page":"1992","DOI":"10.1002\/hep.22862","volume":"49","author":"P Zhou","year":"2009","unstructured":"Zhou P, Hohm S, Olusanya Y, Hess DA, Nolta J: Human progenitor cells with high aldehyde dehydrogenase activity efficiently engraft into damaged liver in a novel model. Hepatology. 2009, 49 (6): 1992-2000. 10.1002\/hep.22862.","journal-title":"Hepatology"},{"issue":"12","key":"446_CR24","doi-asserted-by":"publisher","first-page":"2514","DOI":"10.1161\/ATVBAHA.107.151456","volume":"27","author":"A Woodfin","year":"2007","unstructured":"Woodfin A, Voisin MB, Nourshargh S: PECAM-1: a multi-functional molecule in inflammation and vascular biology. Arterioscler Thromb Vasc Biol. 2007, 27 (12): 2514-2523. 10.1161\/ATVBAHA.107.151456.","journal-title":"Arterioscler Thromb Vasc Biol"},{"issue":"4","key":"446_CR25","doi-asserted-by":"publisher","first-page":"361","DOI":"10.1080\/15476910802482870","volume":"5","author":"B Seliger","year":"2008","unstructured":"Seliger B: Different regulation of MHC class I antigen processing components in human tumors. Journal of immunotoxicology. 2008, 5 (4): 361-367. 10.1080\/15476910802482870.","journal-title":"Journal of immunotoxicology"},{"issue":"21","key":"446_CR26","doi-asserted-by":"publisher","first-page":"5340","DOI":"10.1182\/blood-2008-04-154567","volume":"113","author":"BJ Capoccia","year":"2009","unstructured":"Capoccia BJ, Robson DL, Levac KD, Maxwell DJ, Hohm SA, Neelamkavil MJ, Bell GI, Xenocostas A, Link DC, Piwnica-Worms D: Revascularization of ischemic limbs after transplantation of human bone marrow cells with high aldehyde dehydrogenase activity. Blood. 2009, 113 (21): 5340-5351. 10.1182\/blood-2008-04-154567.","journal-title":"Blood"},{"issue":"4","key":"446_CR27","doi-asserted-by":"publisher","first-page":"367","DOI":"10.1038\/nm0405-367","volume":"11","author":"M Gnecchi","year":"2005","unstructured":"Gnecchi M, He H, Liang OD, Melo LG, Morello F, Mu H, Noiseux N, Zhang L, Pratt RE, Ingwall JS: Paracrine action accounts for marked protection of ischemic heart by Akt-modified mesenchymal stem cells. Nat Med. 2005, 11 (4): 367-368. 10.1038\/nm0405-367.","journal-title":"Nat Med"},{"issue":"3","key":"446_CR28","doi-asserted-by":"publisher","first-page":"700","DOI":"10.1016\/j.bbrc.2007.01.045","volume":"354","author":"Y Iso","year":"2007","unstructured":"Iso Y, Spees JL, Serrano C, Bakondi B, Pochampally R, Song YH, Sobel BE, Delafontaine P, Prockop DJ: Multipotent human stromal cells improve cardiac function after myocardial infarction in mice without long-term engraftment. Biochemical and biophysical research communications. 2007, 354 (3): 700-706. 10.1016\/j.bbrc.2007.01.045.","journal-title":"Biochemical and biophysical research communications"},{"key":"446_CR29","volume-title":"Stem cells and development","author":"CS Sondergaard","year":"2008","unstructured":"Sondergaard CS, Bonde J, Dagnaes-Hansen F, Nielsen JM, Zachar V, Holm M, Hokland P, Pedersen L: Minimal Engraftment of Human CD34(+) Cells Mobilized from Healthy Donors in the Infarcted Heart of Athymic Nude Rats. Stem cells and development. 2008"},{"issue":"12","key":"446_CR30","doi-asserted-by":"publisher","first-page":"1210","DOI":"10.1056\/NEJMoa060186","volume":"355","author":"V Schachinger","year":"2006","unstructured":"Schachinger V, Erbs S, Elsasser A, Haberbosch W, Hambrecht R, Holschermann H, Yu J, Corti R, Mathey DG, Hamm CW: Intracoronary bone marrow-derived progenitor cells in acute myocardial infarction. N Engl J Med. 2006, 355 (12): 1210-1221. 10.1056\/NEJMoa060186.","journal-title":"N Engl J Med"},{"issue":"1","key":"446_CR31","doi-asserted-by":"publisher","first-page":"220","DOI":"10.1634\/stemcells.2006-0243","volume":"25","author":"T Meyerrose","year":"2007","unstructured":"Meyerrose T, De Ugarte D, Hofling A, Herrbrich PE, Cordonnier TD, Shultz LD, Eagon JC, Wirthlin L, Sands MS, Hedrick MA: In vivo distribution of human adipose-derived mesenchymal stem cells in novel xenotransplantation models. Stem Cells. 2007, 25 (1): 220-227. 10.1634\/stemcells.2006-0243.","journal-title":"Stem Cells"},{"issue":"1","key":"446_CR32","doi-asserted-by":"publisher","first-page":"158","DOI":"10.1016\/j.cardiores.2006.03.020","volume":"71","author":"N Ma","year":"2006","unstructured":"Ma N, Ladilov Y, Moebius JM, Ong L, Piechaczek C, David A, Kaminski A, Choi YH, Li W, Egger D: Intramyocardial delivery of human CD133+ cells in a SCID mouse cryoinjury model: Bone marrow vs. cord blood-derived cells. Cardiovasc Res. 2006, 71 (1): 158-169. 10.1016\/j.cardiores.2006.03.020.","journal-title":"Cardiovasc Res"}],"container-title":["Journal of Translational Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1186\/1479-5876-8-24.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,9,1]],"date-time":"2021-09-01T05:22:33Z","timestamp":1630473753000},"score":1,"resource":{"primary":{"URL":"https:\/\/translational-medicine.biomedcentral.com\/articles\/10.1186\/1479-5876-8-24"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2010,3,9]]},"references-count":32,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2010,12]]}},"alternative-id":["446"],"URL":"https:\/\/doi.org\/10.1186\/1479-5876-8-24","relation":{},"ISSN":["1479-5876"],"issn-type":[{"value":"1479-5876","type":"electronic"}],"subject":[],"published":{"date-parts":[[2010,3,9]]},"assertion":[{"value":"28 August 2009","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"9 March 2010","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"9 March 2010","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}}],"article-number":"24"}}