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The effect of topical SiO<jats:sub>2<\/jats:sub>-NP exposure on preexisting ACD has not been studied to date although this reflects a common <jats:italic>in vivo<\/jats:italic> situation. Of particular interest are the potential effects of positively charged <jats:italic>N<\/jats:italic>-(6-aminohexyl)-aminopropyltrimethoxysilane (AHAPS)-functionalized SiO<jats:sub>2<\/jats:sub>-NP which are promising candidates for delivery systems, including gene delivery into the skin. Here, the effects of such AHAPS-functionalized SiO<jats:sub>2<\/jats:sub>-NP (55\u2009\u00b1\u20096\u00a0nm in diameter) were studied in an oxazolone-induced ACD model in SKH1 mice and compared to ACD mice treated with vehicle only. The clinical course of the disease was assessed by monitoring of the transepidermal water loss (TEWL) and the erythema. In histologic and morphometric analyses, the distribution of particles, the degree of inflammation, epidermal thickness, and the inflammatory infiltrate were characterized and quantified by standard and special histological stains as well as immunohistochemistry for CD3+ lymphocytes. To assess possible systemic effects, serum immunoglobulin E (IgE) was determined by enzyme-linked immunosorbent assay. Following administration of AHAPS-SiO<jats:sub>2<\/jats:sub>-NP for five consecutive days, no effects were observed in all clinical, histologic, morphometric, and molecular parameters investigated. In conclusion, positively charged AHAPS-SiO<jats:sub>2<\/jats:sub>-NP seem not to affect the course of ACD during exposure for 5\u00a0days.<\/jats:p>","DOI":"10.1186\/1556-276x-9-524","type":"journal-article","created":{"date-parts":[[2014,9,24]],"date-time":"2014-09-24T01:01:14Z","timestamp":1411520474000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["AHAPS-functionalized silica nanoparticles do not modulate allergic contact dermatitis in mice"],"prefix":"10.1186","volume":"9","author":[{"given":"Anja","family":"Ostrowski","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daniel","family":"Nordmeyer","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lars","family":"Mundhenk","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joachim W","family":"Fluhr","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J\u00fcrgen","family":"Lademann","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christina","family":"Graf","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eckart","family":"R\u00fchl","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Achim D","family":"Gruber","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2014,9,24]]},"reference":[{"key":"2261_CR1","doi-asserted-by":"publisher","first-page":"99","DOI":"10.1007\/s12274-008-8018-3","volume":"1","author":"L Wang","year":"2008","unstructured":"Wang L, Zhao W, Tan W: Bioconjugated silica nanoparticles: development and applications. 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