{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T16:18:08Z","timestamp":1790957888420,"version":"4.1.0"},"reference-count":45,"publisher":"Springer Science and Business Media LLC","issue":"1","content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Virol J"],"published-print":{"date-parts":[[2007,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n          <jats:sec>\n            <jats:title>Background<\/jats:title>\n            <jats:p>Profilins are critical to cytoskeletal dynamics in eukaryotes; however, little is known about their viral counterparts. In this study, a poxviral profilin homolog, ectromelia virus strain Moscow gene 141 (ECTV-PH), was investigated by a variety of experimental and bioinformatics techniques to characterize its interactions with cellular and viral proteins.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Results<\/jats:title>\n            <jats:p>Profilin-like proteins are encoded by all orthopoxviruses sequenced to date, and share over 90% amino acid (aa) identity. Sequence comparisons show highest similarity to mammalian type 1 profilins; however, a conserved 3 aa deletion in mammalian type 3 and poxviral profilins suggests that these homologs may be more closely related. Structural analysis shows that ECTV-PH can be successfully modelled onto both the profilin 1 crystal structure and profilin 3 homology model, though few of the surface residues thought to be required for binding actin, poly(L-proline), and PIP<jats:sub>2<\/jats:sub> are conserved. Immunoprecipitation and mass spectrometry identified two proteins that interact with ECTV-PH within infected cells: alpha-tropomyosin, a 38 kDa cellular actin-binding protein, and the 84 kDa product of vaccinia virus strain Western Reserve (VACV-WR) 148, which is the truncated VACV counterpart of the orthopoxvirus A-type inclusion (ATI) protein. Western and far-western blots demonstrated that the interaction with alpha-tropomyosin is direct, and immunofluorescence experiments suggest that ECTV-PH and alpha-tropomyosin may colocalize to structures that resemble actin tails and cellular protrusions. Sequence comparisons of the poxviral ATI proteins show that although full-length orthologs are only present in cowpox and ectromelia viruses, an ~ 700 aa truncated ATI protein is conserved in over 90% of sequenced orthopoxviruses. Immunofluorescence studies indicate that ECTV-PH localizes to cytoplasmic inclusion bodies formed by both truncated and full-length versions of the viral ATI protein. Furthermore, colocalization of ECTV-PH and truncated ATI protein to protrusions from the cell surface was observed.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Conclusion<\/jats:title>\n            <jats:p>These results suggest a role for ECTV-PH in intracellular transport of viral proteins or intercellular spread of the virus. Broader implications include better understanding of the virus-host relationship and mechanisms by which cells organize and control the actin cytoskeleton.<\/jats:p>\n          <\/jats:sec>","DOI":"10.1186\/1743-422x-4-76","type":"journal-article","created":{"date-parts":[[2007,7,24]],"date-time":"2007-07-24T18:13:55Z","timestamp":1185300835000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["An ectromelia virus profilin homolog interacts with cellular tropomyosin and viral A-type inclusion protein"],"prefix":"10.1186","volume":"4","author":[{"given":"Christine","family":"Butler-Cole","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mary J","family":"Wagner","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Melissa","family":"Da Silva","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gordon D","family":"Brown","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert D","family":"Burke","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chris","family":"Upton","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2007,7,24]]},"reference":[{"key":"289_CR1","doi-asserted-by":"publisher","first-page":"9","DOI":"10.1186\/1471-2180-3-9","volume":"3","author":"V Bitko","year":"2003","unstructured":"Bitko V, Oldenburg A, Garmon NE, Barik S: Profilin is required for viral morphogenesis, syncytium formation, and cell-specific stress fiber induction by respiratory syncytial virus. BMC Microbiol. 2003, 3: 9-10.1186\/1471-2180-3-9.","journal-title":"BMC Microbiol"},{"key":"289_CR2","doi-asserted-by":"publisher","first-page":"211","DOI":"10.1016\/j.febslet.2006.12.013","volume":"581","author":"D Polet","year":"2007","unstructured":"Polet D, Lambrechts A, Vandepoele K, Vandekerckhove J, Ampe C: On the origin and evolution of vertebrate and viral profilins. FEBS Lett. 2007, 581: 211-217. 10.1016\/j.febslet.2006.12.013.","journal-title":"FEBS Lett"},{"key":"289_CR3","doi-asserted-by":"publisher","first-page":"111","DOI":"10.1023\/A:1008140615106","volume":"21","author":"JJ Bugert","year":"2000","unstructured":"Bugert JJ, Darai G: Poxvirus homologues of cellular genes. Virus Genes. 2000, 21: 111-133. 10.1023\/A:1008140615106.","journal-title":"Virus Genes"},{"key":"289_CR4","doi-asserted-by":"publisher","first-page":"695","DOI":"10.1111\/j.1462-5822.2004.00423.x","volume":"6","author":"JB Johnston","year":"2004","unstructured":"Johnston JB, McFadden G: Technical knockout: understanding poxvirus pathogenesis by selectively deleting viral immunomodulatory genes. Cell Microbiol. 2004, 6: 695-705. 10.1111\/j.1462-5822.2004.00423.x.","journal-title":"Cell Microbiol"},{"key":"289_CR5","doi-asserted-by":"publisher","first-page":"6093","DOI":"10.1128\/JVI.77.11.6093-6100.2003","volume":"77","author":"JB Johnston","year":"2003","unstructured":"Johnston JB, McFadden G: Poxvirus immunomodulatory strategies: current perspectives. J Virol. 2003, 77: 6093-6100. 10.1128\/JVI.77.11.6093-6100.2003.","journal-title":"J Virol"},{"key":"289_CR6","unstructured":"Viral Orthologous Clusters database. [http:\/\/www.virology.ca]"},{"key":"289_CR7","doi-asserted-by":"publisher","first-page":"7590","DOI":"10.1128\/JVI.77.13.7590-7600.2003","volume":"77","author":"C Upton","year":"2003","unstructured":"Upton C, Slack S, Hunter AL, Ehlers A, Roper RL: Poxvirus orthologous clusters: toward defining the minimum essential poxvirus genome. J Virol. 2003, 77: 7590-7600. 10.1128\/JVI.77.13.7590-7600.2003.","journal-title":"J Virol"},{"key":"289_CR8","doi-asserted-by":"publisher","first-page":"461","DOI":"10.1016\/j.tcb.2004.07.003","volume":"14","author":"W Witke","year":"2004","unstructured":"Witke W: The role of profilin complexes in cell motility and other cellular processes. Trends Cell Biol. 2004, 14: 461-469. 10.1016\/j.tcb.2004.07.003.","journal-title":"Trends Cell Biol"},{"key":"289_CR9","doi-asserted-by":"publisher","first-page":"197","DOI":"10.1016\/j.tibs.2006.02.006","volume":"31","author":"EG Yarmola","year":"2006","unstructured":"Yarmola EG, Bubb MR: Profilin: emerging concepts and lingering misconceptions. Trends Biochem Sci. 2006, 31: 197-205. 10.1016\/j.tibs.2006.02.006.","journal-title":"Trends Biochem Sci"},{"key":"289_CR10","doi-asserted-by":"publisher","first-page":"439","DOI":"10.1016\/j.bbamem.2006.12.012","volume":"1768","author":"PDJ Moens","year":"2007","unstructured":"Moens PDJ, Bagatolli LA: Profilin binding to sub-micellar concentrations of phosphatidylinositol (4,5) bisphosphate and phosphatidylinositol (3,4,5) trisphosphate. Biochim Biophys Acta. 2007, 1768: 439-449. 10.1016\/j.bbamem.2006.12.012.","journal-title":"Biochim Biophys Acta"},{"key":"289_CR11","doi-asserted-by":"publisher","first-page":"10815","DOI":"10.1021\/bi00201a032","volume":"33","author":"LM Machesky","year":"1994","unstructured":"Machesky LM, Cole NB, Moss B, Pollard TD: Vaccinia virus expresses a novel profilin with a higher affinity for polyphosphoinositides than actin. Biochemistry. 1994, 33: 10815-10824. 10.1021\/bi00201a032.","journal-title":"Biochemistry"},{"key":"289_CR12","doi-asserted-by":"crossref","first-page":"937","DOI":"10.1091\/mbc.1.12.937","volume":"1","author":"LM Machesky","year":"1990","unstructured":"Machesky LM, Goldschmidt-Clermont PJ, Pollard TD: Theaffinities of human platelet and Acanthamoeba profilin isoforms for polyphosphoinositides account for their relative abilities to inhibit phospholipase C. Cell Regul. 1990, 1: 937-950.","journal-title":"Cell Regul"},{"key":"289_CR13","doi-asserted-by":"publisher","first-page":"320","DOI":"10.1016\/j.tim.2005.05.010","volume":"13","author":"K D\u00f6hner","year":"2005","unstructured":"D\u00f6hner K, Nagel CH, Sodeik B: Viral stop-and-go along microtubules: taking a ride with dynein and kinesins. Trends Microbiol. 2005, 13: 320-327. 10.1016\/j.tim.2005.05.010.","journal-title":"Trends Microbiol"},{"key":"289_CR14","doi-asserted-by":"publisher","first-page":"387","DOI":"10.1111\/j.1462-5822.2005.00679.x","volume":"8","author":"K Radtke","year":"2006","unstructured":"Radtke K, D\u00f6hner K, Sodeik B: Viral interactions with the cytoskeleton: a hitchhiker's guide to the cell. Cell Microbiol. 2006, 8: 387-400. 10.1111\/j.1462-5822.2005.00679.x.","journal-title":"Cell Microbiol"},{"key":"289_CR15","doi-asserted-by":"publisher","first-page":"189","DOI":"10.1016\/j.virusres.2004.08.015","volume":"106","author":"GL Smith","year":"2004","unstructured":"Smith GL, Law M: The exit of vaccinia virus from infected cells. Virus Res. 2004, 106: 189-197. 10.1016\/j.virusres.2004.08.015.","journal-title":"Virus Res"},{"key":"289_CR16","doi-asserted-by":"publisher","first-page":"4802","DOI":"10.1128\/JVI.75.10.4802-4813.2001","volume":"75","author":"BM Ward","year":"2001","unstructured":"Ward BM, Moss B: Visualization of intracellular movement of vaccinia virus virions containing a green fluorescent protein-B5R membrane protein chimera. J Virol. 2001, 75: 4802-4813. 10.1128\/JVI.75.10.4802-4813.2001.","journal-title":"J Virol"},{"key":"289_CR17","doi-asserted-by":"publisher","first-page":"3381","DOI":"10.1093\/nar\/gkg520","volume":"31","author":"T Schwede","year":"2003","unstructured":"Schwede T, Kopp J, Guex N, Peitsch MC: SWISS-MODEL: Anautomated protein homology-modeling server. Nucleic Acids Res. 2003, 31: 3381-3385. 10.1093\/nar\/gkg520.","journal-title":"Nucleic Acids Res"},{"key":"289_CR18","doi-asserted-by":"publisher","first-page":"480","DOI":"10.1006\/jmbi.1994.1522","volume":"241","author":"AA Fedorov","year":"1994","unstructured":"Fedorov AA, Pollard TD, Almo SC: Purification, characterization and crystallization of human platelet profilin expressed in Escherichia coli. J Mol Biol. 1994, 241: 480-482. 10.1006\/jmbi.1994.1522.","journal-title":"J Mol Biol"},{"key":"289_CR19","doi-asserted-by":"publisher","first-page":"1271","DOI":"10.1006\/jmbi.1999.3318","volume":"294","author":"IM Nodelman","year":"1999","unstructured":"Nodelman IM, Bowman GD, Lindberg U, Schutt CE: X-ray structure determination of human profilin II: A comparative structural analysis of human profilins. J Mol Biol. 1999, 294: 1271-1285. 10.1006\/jmbi.1999.3318.","journal-title":"J Mol Biol"},{"issue":"Suppl 6","key":"289_CR20","doi-asserted-by":"publisher","first-page":"524","DOI":"10.1002\/prot.10529","volume":"53","author":"D Chivian","year":"2003","unstructured":"Chivian D, Kim DE, Malmstr\u00f6m L, Bradley P, Robertson T, Murphy P, Strauss CEM, Bonneau R, Rohl CA, Baker D: Automated prediction of CASP-5 structures using the Robetta server. Proteins. 2003, 53 (Suppl 6): 524-533. 10.1002\/prot.10529.","journal-title":"Proteins"},{"issue":"Suppl 7","key":"289_CR21","doi-asserted-by":"publisher","first-page":"157","DOI":"10.1002\/prot.20733","volume":"61","author":"D Chivian","year":"2005","unstructured":"Chivian D, Kim DE, Malmstr\u00f6m L, Schonbrun J, Rohl CA, Baker D: Prediction of CASP6 structures using automated Robetta protocols. Proteins. 2005, 61 (Suppl 7): 157-166. 10.1002\/prot.20733.","journal-title":"Proteins"},{"key":"289_CR22","doi-asserted-by":"publisher","first-page":"W526","DOI":"10.1093\/nar\/gkh468","volume":"32","author":"DE Kim","year":"2004","unstructured":"Kim DE, Chivian D, Baker D: Protein structure prediction and analysis using the Robetta server. Nucleic Acids Res. 2004, 32: W526-W531. 10.1093\/nar\/gkh468.","journal-title":"Nucleic Acids Res"},{"key":"289_CR23","doi-asserted-by":"publisher","first-page":"14027","DOI":"10.1021\/bi961878z","volume":"35","author":"PJ Lu","year":"1996","unstructured":"Lu PJ, Shieh WR, Rhee SG, Yin HL, Chen CS: Lipid products of phosphoinositide 3-kinase bind human profilin with high affinity. Biochemistry. 1996, 35: 14027-14034. 10.1021\/bi961878z.","journal-title":"Biochemistry"},{"key":"289_CR24","doi-asserted-by":"publisher","first-page":"21114","DOI":"10.1074\/jbc.270.36.21114","volume":"270","author":"RH Sohn","year":"1995","unstructured":"Sohn RH, Chen J, Koblan KS, Bray PF, Goldschmidt-Clermont PJ: Localization of a binding site for phosphatidylinositol 4,5-bisphosphate on human profilin. J Biol Chem. 1995, 270: 21114-21120. 10.1074\/jbc.270.36.21114.","journal-title":"J Biol Chem"},{"issue":"Pt 8","key":"289_CR25","doi-asserted-by":"publisher","first-page":"1679","DOI":"10.1099\/0022-1317-74-8-1679","volume":"74","author":"H Meyer","year":"1993","unstructured":"Meyer H, Rziha HJ: Characterization of the gene encoding the A-type inclusion protein of camelpox virus and sequence comparison with other orthopoxviruses. J Gen Virol. 1993, 74 (Pt 8): 1679-1684.","journal-title":"J Gen Virol"},{"key":"289_CR26","doi-asserted-by":"publisher","first-page":"147","DOI":"10.1016\/S0168-1702(99)00013-1","volume":"60","author":"KH Martin","year":"1999","unstructured":"Martin KH, Franke CA, Hruby DE: Novel acylation of poxvirus A-type inclusion proteins. Virus Res. 1999, 60: 147-157. 10.1016\/S0168-1702(99)00013-1.","journal-title":"Virus Res"},{"key":"289_CR27","doi-asserted-by":"crossref","first-page":"3372","DOI":"10.1128\/jvi.70.6.3372-3377.1996","volume":"70","author":"D Ulaeto","year":"1996","unstructured":"Ulaeto D, Grosenbach D, Hruby DE: The vaccinia virus 4c and A-type inclusion proteins are specific markers for the intracellular mature virus particle. J Virol. 1996, 70: 3372-3377.","journal-title":"J Virol"},{"key":"289_CR28","doi-asserted-by":"publisher","first-page":"174","DOI":"10.1016\/0042-6822(86)90119-4","volume":"149","author":"DD Patel","year":"1986","unstructured":"Patel DD, Pickup DJ, Joklik WK: Isolation of cowpox virus A-type inclusions and characterization of their major protein component. Virology. 1986, 149: 174-189. 10.1016\/0042-6822(86)90119-4.","journal-title":"Virology"},{"key":"289_CR29","doi-asserted-by":"publisher","first-page":"125","DOI":"10.1007\/BF01703611","volume":"8","author":"N Osterrieder","year":"1994","unstructured":"Osterrieder N, Meyer H, Pfeffer M: Characterization of the gene encoding the A-type inclusion body protein of mousepox virus. Virus Genes. 1994, 8: 125-135. 10.1007\/BF01703611.","journal-title":"Virus Genes"},{"key":"289_CR30","doi-asserted-by":"publisher","first-page":"333","DOI":"10.1016\/j.tcb.2005.04.007","volume":"15","author":"PW Gunning","year":"2005","unstructured":"Gunning PW, Schevzov G, Kee AJ, Hardeman EC: Tropomyosin isoforms: divining rods for actin cytoskeleton function. Trends Cell Biol. 2005, 15: 333-341. 10.1016\/j.tcb.2005.04.007.","journal-title":"Trends Cell Biol"},{"key":"289_CR31","doi-asserted-by":"publisher","first-page":"35","DOI":"10.1016\/j.mib.2004.12.013","volume":"8","author":"E Gouin","year":"2005","unstructured":"Gouin E, Welch MD, Cossart P: Actin-based motility of intracellular pathogens. Curr Opin Microbiol. 2005, 8: 35-45. 10.1016\/j.mib.2004.12.013.","journal-title":"Curr Opin Microbiol"},{"key":"289_CR32","doi-asserted-by":"crossref","first-page":"4598","DOI":"10.1128\/jvi.65.9.4598-4608.1991","volume":"65","author":"R Blasco","year":"1991","unstructured":"Blasco R, Cole NB, Moss B: Sequence analysis, expression, and deletion of a vaccinia virus gene encoding a homolog of profilin, a eukaryotic actin-binding protein. J Virol. 1991, 65: 4598-4608.","journal-title":"J Virol"},{"key":"289_CR33","doi-asserted-by":"publisher","first-page":"96","DOI":"10.1186\/1471-2105-5-96","volume":"5","author":"R Brodie","year":"2004","unstructured":"Brodie R, Smith AJ, Roper RL, Tcherepanov V, Upton C: Base-By-Base: single nucleotide-level analysis of whole viral genome alignments. BMC Bioinformatics. 2004, 5: 96-10.1186\/1471-2105-5-96.","journal-title":"BMC Bioinformatics"},{"key":"289_CR34","doi-asserted-by":"publisher","first-page":"205","DOI":"10.1006\/jmbi.2000.4042","volume":"302","author":"C Notredame","year":"2000","unstructured":"Notredame C, Higgins DG, Heringa J: T-Coffee: A novel method for fast and accurate multiple sequence alignment. J Mol Biol. 2000, 302: 205-217. 10.1006\/jmbi.2000.4042.","journal-title":"J Mol Biol"},{"key":"289_CR35","first-page":"164","volume":"5","author":"Felsenstein","year":"1989","unstructured":"Felsenstein : PHYLIP \u2013 Phylogeny Inference Package (Version3.2). Cladistics. 1989, 5: 164-166.","journal-title":"Cladistics"},{"key":"289_CR36","first-page":"275","volume":"8","author":"DT Jones","year":"1992","unstructured":"Jones DT, Taylor WR, Thornton JM: The rapid generation of mutation data matrices from protein sequences. Comput Appl Biosci. 1992, 8: 275-282.","journal-title":"Comput Appl Biosci"},{"key":"289_CR37","unstructured":"The Xfig Drawing Program for the X Window System. [http:\/\/www.xfig.org]"},{"key":"289_CR38","doi-asserted-by":"publisher","first-page":"403","DOI":"10.1016\/S0022-2836(05)80360-2","volume":"215","author":"SF Altschul","year":"1990","unstructured":"Altschul SF, Gish W, Miller W, Myers EW, Lipman DJ: Basic local alignment search tool. J Mol Biol. 1990, 215: 403-410.","journal-title":"J Mol Biol"},{"key":"289_CR39","doi-asserted-by":"publisher","first-page":"450","DOI":"10.1002\/pro.5560040312","volume":"4","author":"WJ Metzler","year":"1995","unstructured":"Metzler WJ, Farmer BT, Constantine KL, Friedrichs MS, Lavoie T, Mueller L: Refined solution structure of human profilin I. Protein Sci. 1995, 4: 450-459.","journal-title":"Protein Sci"},{"key":"289_CR40","doi-asserted-by":"publisher","first-page":"607","DOI":"10.1006\/jmbi.1996.0602","volume":"263","author":"JK Chik","year":"1996","unstructured":"Chik JK, Lindberg U, Schutt CE: The structure of an open state of beta-actin at 2.65 A resolution. J Mol Biol. 1996, 263: 607-623. 10.1006\/jmbi.1996.0602.","journal-title":"J Mol Biol"},{"key":"289_CR41","doi-asserted-by":"publisher","first-page":"1605","DOI":"10.1002\/jcc.20084","volume":"25","author":"EF Pettersen","year":"2004","unstructured":"Pettersen EF, Goddard TD, Huang CC, Couch GS, Greenblatt DM, Meng EC, Ferrin TE: UCSF Chimera\u2013a visualization system for exploratory research and analysis. J Comput Chem. 2004, 25: 1605-1612. 10.1002\/jcc.20084.","journal-title":"J Comput Chem"},{"key":"289_CR42","doi-asserted-by":"publisher","first-page":"666","DOI":"10.1038\/10722","volume":"6","author":"NM Mahoney","year":"1999","unstructured":"Mahoney NM, Rozwarski DA, Fedorov E, Fedorov AA, Almo SC: Profilin binds proline-rich ligands in two distinct amide backbone orientations. Nat Struct Biol. 1999, 6: 666-671. 10.1038\/10722.","journal-title":"Nat Struct Biol"},{"key":"289_CR43","doi-asserted-by":"publisher","first-page":"8122","DOI":"10.1073\/pnas.83.21.8122","volume":"83","author":"TR Fuerst","year":"1986","unstructured":"Fuerst TR, Niles EG, Studier FW, Moss B: Eukaryotic transient-expression system based on recombinant vaccinia virus that synthesizes bacteriophage T7 RNA polymerase. Proc Natl Acad Sci USA. 1986, 83: 8122-8126. 10.1073\/pnas.83.21.8122.","journal-title":"Proc Natl Acad Sci USA"},{"key":"289_CR44","doi-asserted-by":"publisher","first-page":"3551","DOI":"10.1002\/(SICI)1522-2683(19991201)20:18<3551::AID-ELPS3551>3.0.CO;2-2","volume":"20","author":"DN Perkins","year":"1999","unstructured":"Perkins DN, Pappin DJ, Creasy DM, Cottrell JS: Probability-based protein identification by searching sequence databases using mass spectrometry data. Electrophoresis. 1999, 20: 3551-3567. 10.1002\/(SICI)1522-2683(19991201)20:18<3551::AID-ELPS3551>3.0.CO;2-2.","journal-title":"Electrophoresis"},{"key":"289_CR45","doi-asserted-by":"publisher","first-page":"419","DOI":"10.1016\/j.pep.2004.06.034","volume":"37","author":"G Rea","year":"2004","unstructured":"Rea G, Iacovacci P, Ferrante P, Zelli M, Brunetto B, Lamba D, Boffi A, Pini C, Federico R: Refolding of the Cupressus arizonica major pollen allergen Cup a1.02 overexpressed in Escherichia coli. Protein Expr Purif. 2004, 37: 419-425. 10.1016\/j.pep.2004.06.034.","journal-title":"Protein Expr Purif"}],"container-title":["Virology Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1186\/1743-422X-4-76.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,9,1]],"date-time":"2021-09-01T07:35:12Z","timestamp":1630481712000},"score":1,"resource":{"primary":{"URL":"https:\/\/virologyj.biomedcentral.com\/articles\/10.1186\/1743-422X-4-76"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2007,7,24]]},"references-count":45,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2007,12]]}},"alternative-id":["289"],"URL":"https:\/\/doi.org\/10.1186\/1743-422x-4-76","relation":{},"ISSN":["1743-422X"],"issn-type":[{"value":"1743-422X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2007,7,24]]},"assertion":[{"value":"14 May 2007","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"24 July 2007","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"24 July 2007","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}}],"article-number":"76"}}