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There was no cross-reaction with porcine circovirus 2 (PCV2), porcine reproductive and respiratory syndrome virus (PRRSV), pseudorabies virus (PRV), classical swine fever virus (CSFV), or Japanese encephalitis virus (JEV). The assay was specific and reproducible. In 41 clinical samples, PPV was detected in 32 samples with the real-time PCR assay and in only 11 samples with a conventional PCR assay. The real-time assay using the TaqMan-system can therefore be practically used for studying the epidemiology and management of PPV.<\/jats:p>","DOI":"10.1186\/1743-422x-7-353","type":"journal-article","created":{"date-parts":[[2010,12,2]],"date-time":"2010-12-02T19:14:24Z","timestamp":1291317264000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":39,"title":["Detection of porcine parvovirus using a taqman-based real-time pcr with primers and probe designed for the NS1 gene"],"prefix":"10.1186","volume":"7","author":[{"given":"Cuiping","family":"Song","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chao","family":"Zhu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chaofan","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shangjin","family":"Cui","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2010,12,2]]},"reference":[{"key":"1097_CR1","first-page":"93","volume-title":"Diseases of Swine","author":"WL Mengeling","year":"2006","unstructured":"Mengeling WL: Porcine parvovirus. 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