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These assays however are either time-consuming and lack convenient readouts, or they are rather insensitive and restricted to IFN from a particular host species.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Results<\/jats:title>\n            <jats:p>An IFN-sensitive, <jats:italic>Renilla<\/jats:italic> luciferase-expressing Rift Valley fever virus (RVFV-Ren) was generated using reverse genetics. Human, murine and avian cells were tested for their susceptibility to RVFV-Ren after treatment with species-specific IFNs. RVFV-Ren was able to infect cells of all three species, and IFN-mediated inhibition of viral reporter activity occurred in a dose-dependent manner. The sensitivity limit was found to be 1 U\/ml IFN, and comparison with a standard curve allowed to determine the activity of an unknown sample.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Conclusions<\/jats:title>\n            <jats:p>RVFV-Ren replicates in cells of several species and is highly sensitive to pre-treatment with IFN. These properties allowed the development of a rapid, sensitive, and species-independent antiviral assay with a convenient luciferase-based readout.<\/jats:p>\n          <\/jats:sec>","DOI":"10.1186\/1743-422x-7-50","type":"journal-article","created":{"date-parts":[[2010,2,26]],"date-time":"2010-02-26T19:15:19Z","timestamp":1267211719000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":32,"title":["Species-independent bioassay for sensitive quantification of antiviral type I interferons"],"prefix":"10.1186","volume":"7","author":[{"given":"Thomas","family":"Kuri","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matthias","family":"Habjan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicola","family":"Penski","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Friedemann","family":"Weber","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2010,2,26]]},"reference":[{"key":"795_CR1","doi-asserted-by":"publisher","first-page":"119","DOI":"10.1016\/j.virol.2005.09.024","volume":"344","author":"O Haller","year":"2006","unstructured":"Haller O, Kochs G, Weber F: The interferon response circuit: induction and suppression by pathogenic viruses. 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