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In this study a recombinant baculovirus (vSynYFE) containing the envelope gene (<jats:italic>env<\/jats:italic>) of the 17D vaccine strain of yellow fever virus was constructed and the recombinant protein antigenicity was tested.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Results<\/jats:title>\n            <jats:p>Insect cells infected with vSynYFE showed syncytium formation, which is a cytopathic effect characteristic of flavivirus infection and expressed a polypeptide of around 54 kDa, which corresponds to the expected size of the recombinant E protein. Furthermore, the recombinant E protein expression was also confirmed by fluorescence microscopy of vSynYFE-infected insect cells. Total vSynYFE-infected insect extracts used as antigens detected the presence of antibodies for yellow fever virus in human sera derived from yellow fever-infected patients in an immunoassay and did not cross react with sera from dengue virus-infected patients.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Conclusions<\/jats:title>\n            <jats:p>The E protein expressed by the recombinant baculovirus in insect cells is antigenically similar to the wild protein and it may be useful for different medical applications, from improved diagnosis of the disease to source of antigens for the development of a subunit vaccine.<\/jats:p>\n          <\/jats:sec>","DOI":"10.1186\/1743-422x-8-261","type":"journal-article","created":{"date-parts":[[2011,5,27]],"date-time":"2011-05-27T18:23:22Z","timestamp":1306520602000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Yellow fever virus envelope protein expressed in insect cells is capable of syncytium formation in lepidopteran cells and could be used for immunodetection of YFV in human sera"],"prefix":"10.1186","volume":"8","author":[{"given":"Maria CES","family":"Barros","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tatiane GCM","family":"Galasso","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ant\u00f4nio JM","family":"Chaib","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicolas","family":"Degallier","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tatsuya","family":"Nagata","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bergmann M","family":"Ribeiro","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2011,5,27]]},"reference":[{"issue":"3","key":"1356_CR1","doi-asserted-by":"publisher","first-page":"244","DOI":"10.1590\/S1415-790X2002000300004","volume":"5","author":"PFC Vasconcelos","year":"2002","unstructured":"Vasconcelos PFC: Febre amarela: reflex\u00f5es sobre a doen\u00e7a, as perspectivas para o s\u00e9culo XXI e o risco da reurbaniza\u00e7\u00e3o. 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