{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T12:14:28Z","timestamp":1790511268066,"version":"4.1.0"},"reference-count":28,"publisher":"Springer Science and Business Media LLC","issue":"1","content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Virol J"],"published-print":{"date-parts":[[2012,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n          <jats:sec>\n            <jats:title>Background<\/jats:title>\n            <jats:p>Dengue virus (DENV), the causative agent of human Dengue hemorrhagic fever, is a mosquito-borne virus found in tropical and sub-tropical regions around the world. Vaccines against DENV are currently unavailable. Guanylate-binding protein 1 (GBP1) is one of the Interferon (IFN) stimulated genes (ISGs) and has been shown important for host immune defense against various pathogens. However, the role of GBP1 during DENV infection remains unclarified. In this study, we evaluated the relevance of GBP1 to DENV infection in <jats:italic>in vitro<\/jats:italic> model.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Findings<\/jats:title>\n            <jats:p>Quantitative RT-PCR (qRT-PCR) and Western blot showed that the expression of mouse <jats:italic>Gbp1<\/jats:italic> was dramatically upregulated in DENV-infected RAW264.7 cells. The intracellular DENV loads were significantly higher in <jats:italic>Gbp1<\/jats:italic> silenced cells compared with controls. The expression levels of selective anti-viral cytokines were decreased in <jats:italic>Gbp1<\/jats:italic> siRNA treated cells, while the transcription factor activity of NF-\u03baB was impaired upon <jats:italic>GBP1<\/jats:italic> silencing during infection.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Conclusions<\/jats:title>\n            <jats:p>Our data suggested that GBP1 plays an antiviral role during DENV infection.<\/jats:p>\n          <\/jats:sec>","DOI":"10.1186\/1743-422x-9-292","type":"journal-article","created":{"date-parts":[[2012,11,27]],"date-time":"2012-11-27T11:14:27Z","timestamp":1354014867000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":67,"title":["Guanylate-binding protein 1 participates in cellular antiviral response to dengue virus"],"prefix":"10.1186","volume":"9","author":[{"given":"Wen","family":"Pan","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiangyang","family":"Zuo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tingting","family":"Feng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaohong","family":"Shi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianfeng","family":"Dai","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2012,11,27]]},"reference":[{"key":"1932_CR1","doi-asserted-by":"publisher","first-page":"7221","DOI":"10.1016\/j.vaccine.2011.07.022","volume":"29","author":"J Whitehorn","year":"2011","unstructured":"Whitehorn J, Simmons CP: The pathogenesis of dengue. 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