{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,8]],"date-time":"2026-02-08T04:24:34Z","timestamp":1770524674258,"version":"3.49.0"},"reference-count":35,"publisher":"Springer Science and Business Media LLC","issue":"1","content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Part Fibre Toxicol"],"published-print":{"date-parts":[[2012,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Single-walled carbon nanotubes (SWCNT) trigger pronounced inflammation and fibrosis in the lungs of mice following administration via pharyngeal aspiration or inhalation. Human exposure to SWCNT in an occupational setting may occur in conjunction with infections and this could yield enhanced or suppressed responses to the offending agent. Here, we studied whether the sequential exposure to SWCNT via pharyngeal aspiration and infection of mice with the ubiquitous intracellular parasite<jats:italic>Toxoplasma gondii<\/jats:italic>would impact on the immune response of the host against the parasite.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>C57BL\/6 mice were pre-exposed by pharyngeal administration of SWCNT (80\u2009+\u200980\u2009\u03bcg\/mouse) for two consecutive days followed by intravenous injection with either 1x10<jats:sup>3<\/jats:sup>or 1x10<jats:sup>4<\/jats:sup>green fluorescence protein and luciferase-expressing<jats:italic>T. gondii<\/jats:italic>tachyzoites. The dissemination of<jats:italic>T. gondii<\/jats:italic>was monitored by<jats:italic>in vivo<\/jats:italic>bioluminescence imaging in real time for 7\u2009days and by plaque formation<jats:italic>.<\/jats:italic>The inflammatory response was analysed in bronchoalveolar lavage (BAL) fluid, and by assessment of morphological changes and immune responses in lung and spleen.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>There were no differences in parasite distribution between mice only inoculated with<jats:italic>T. gondii<\/jats:italic>or those mice pre-exposed for 2\u2009days to SWCNT before parasite inoculum. Lung and spleen histology and inflammation markers in BAL fluid reflected the effects of SWCNT exposure and<jats:italic>T. gondii<\/jats:italic>injection, respectively. We also noted that CD11c positive dendritic cells but not F4\/80 positive macrophages retained SWCNT in the lungs 9\u2009days after pharyngeal aspiration. However, co-localization of<jats:italic>T. gondii<\/jats:italic>with CD11c or F4\/80 positive cells could not be observed in lungs or spleen. Pre-exposure to SWCNT did not affect the splenocyte response to<jats:italic>T. gondii.<\/jats:italic><\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Taken together, our data indicate that pre-exposure to SWCNT does not enhance or suppress the early immune response to<jats:italic>T. gondii<\/jats:italic>in mice.<\/jats:p><\/jats:sec>","DOI":"10.1186\/1743-8977-9-16","type":"journal-article","created":{"date-parts":[[2012,5,31]],"date-time":"2012-05-31T17:15:24Z","timestamp":1338484524000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["Pulmonary exposure to single-walled carbon nanotubes does not affect the early immune response against Toxoplasma gondii"],"prefix":"10.1186","volume":"9","author":[{"given":"Linda","family":"Swedin","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Romanico","family":"Arrighi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Britta","family":"Andersson-Willman","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ashley","family":"Murray","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yunying","family":"Chen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mikael C I","family":"Karlsson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Susanna Kumlien","family":"Geor\u00e9n","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alexey V","family":"Tkach","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anna A","family":"Shvedova","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bengt","family":"Fadeel","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Antonio","family":"Barragan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Annika","family":"Scheynius","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2012,5,23]]},"reference":[{"issue":"2","key":"205_CR1","doi-asserted-by":"publisher","first-page":"192","DOI":"10.1016\/j.pharmthera.2008.10.009","volume":"121","author":"AA Shvedova","year":"2009","unstructured":"Shvedova AA, Kisin ER, Porter D, Schulte P, Kagan VE, Fadeel B, Castranova V: Mechanisms of pulmonary toxicity and medical applications of carbon nanotubes: Two faces of Janus? 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