{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,6]],"date-time":"2026-11-06T17:09:15Z","timestamp":1793984955338,"version":"4.8.0"},"reference-count":56,"publisher":"SAGE Publications","license":[{"start":{"date-parts":[[2014,1,1]],"date-time":"2014-01-01T00:00:00Z","timestamp":1388534400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Mol Pain"],"published-print":{"date-parts":[[2014,1,1]]},"abstract":"<jats:sec><jats:title>Background:<\/jats:title><jats:p> Tissue acidosis is effective in causing chronic muscle pain. However, how muscle nociceptors contribute to the transition from acute to chronic pain is largely unknown. <\/jats:p><\/jats:sec><jats:sec><jats:title>Results:<\/jats:title><jats:p> Here we showed that a single intramuscular acid injection induced a priming effect on muscle nociceptors of mice. The primed muscle nociceptors were plastic and permitted the development of long-lasting chronic hyperalgesia induced by a second acid insult. The plastic changes of muscle nociceptors were modality-specific and required the activation of acid-sensing ion channel 3 (***ASIC3) or transient receptor potential cation channel V1 (TRPV1). Activation of ASIC3 was associated with increased activity of tetrodotoxin (TTX)-sensitive voltage-gated sodium channels but not protein kinase C\u03b5 (PKC\u03b5) in isolectin B4 (IB4)-negative muscle nociceptors. In contrast, increased activity of TTX-resistant voltage-gated sodium channels with ASIC3 or TRPV1 activation in Na<jats:sub>V<\/jats:sub>1.8-positive muscle nociceptors was required for the development of chronic hyperalgesia. Accordingly, compared to wild type mice, Na<jats:sub>V<\/jats:sub>1.8-null mice showed briefer acid-induced hyperalgesia (5 days vs. &gt;27 days). <\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion:<\/jats:title><jats:p> ASIC3 activation may manifest a new type of nociceptor priming in IB4-negative muscle nociceptors. The activation of ASIC3 and TRPV1 as well as enhanced Na<jats:sub>V<\/jats:sub>1.8 activity are essential for the development of long-lasting hyperalgesia in acid-induced, chronic, widespread muscle pain. <\/jats:p><\/jats:sec>","DOI":"10.1186\/1744-8069-10-40","type":"journal-article","created":{"date-parts":[[2014,6,23]],"date-time":"2014-06-23T11:03:22Z","timestamp":1403521402000},"update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":28,"title":["Roles of ASIC3, TRPV1, and Na<sub>V<\/sub>1.8 in the Transition from Acute to Chronic Pain in a Mouse Model of Fibromyalgia"],"prefix":"10.1177","volume":"10","author":[{"given":"Wei-Nan","family":"Chen","sequence":"first","affiliation":[{"name":"Graduate Institute of Life Sciences, National Defense Medical Center, Taipei 114, Taiwan"},{"name":"Institute of Biomedical Sciences, Academia Sinica, 128 Academia Road, Section 2, Taipei 115, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cheng-Han","family":"Lee","sequence":"additional","affiliation":[{"name":"Institute of Biomedical Sciences, Academia Sinica, 128 Academia Road, Section 2, Taipei 115, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shing-Hong","family":"Lin","sequence":"additional","affiliation":[{"name":"Graduate Institute of Life Sciences, National Defense Medical Center, Taipei 114, Taiwan"},{"name":"Institute of Biomedical Sciences, Academia Sinica, 128 Academia Road, Section 2, Taipei 115, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chia-Wen","family":"Wong","sequence":"additional","affiliation":[{"name":"Institute of Biomedical Sciences, Academia Sinica, 128 Academia Road, Section 2, Taipei 115, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei-Hsin","family":"Sun","sequence":"additional","affiliation":[{"name":"Department of Life Sciences, National Central University, Jhongli 32054, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John N","family":"Wood","sequence":"additional","affiliation":[{"name":"Wolfson Institute for Biomedical Research, Molecular Nociception Group, University College London, London WC1E 6BT, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chin-Cheng","family":"Chen","sequence":"additional","affiliation":[{"name":"Graduate Institute of Life Sciences, National Defense Medical Center, Taipei 114, Taiwan"},{"name":"Institute of Biomedical Sciences, Academia Sinica, 128 Academia Road, Section 2, Taipei 115, Taiwan"},{"name":"Taiwan Mouse Clinic\u2014National Comprehensive Mouse Phenotyping and Drug Testing Center, Academia Sinica, 128 Academia Road, Section 2, Taipei 115, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2014,1,1]]},"reference":[{"key":"bibr1-1744-8069-10-40","first-page":"214","volume":"105","author":"Mense S","year":"2008","journal-title":"Dtsch Arztebl Int"},{"key":"bibr2-1744-8069-10-40","doi-asserted-by":"publisher","DOI":"10.1179\/106698111X13129729551903"},{"key":"bibr3-1744-8069-10-40","doi-asserted-by":"publisher","DOI":"10.1007\/s11916-008-0057-7"},{"key":"bibr4-1744-8069-10-40","doi-asserted-by":"publisher","DOI":"10.1016\/j.berh.2010.01.010"},{"key":"bibr5-1744-8069-10-40","doi-asserted-by":"publisher","DOI":"10.1016\/j.tins.2009.07.007"},{"key":"bibr6-1744-8069-10-40","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.5138-11.2012"},{"key":"bibr7-1744-8069-10-40","doi-asserted-by":"publisher","DOI":"10.1016\/S0896-6273(03)00473-2"},{"key":"bibr8-1744-8069-10-40","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuroscience.2010.04.082"},{"key":"bibr9-1744-8069-10-40","doi-asserted-by":"publisher","DOI":"10.1016\/j.pain.2008.08.014"},{"key":"bibr10-1744-8069-10-40","doi-asserted-by":"publisher","DOI":"10.1016\/0304-3940(96)12576-3"},{"key":"bibr11-1744-8069-10-40","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2010.09.029"},{"key":"bibr12-1744-8069-10-40","doi-asserted-by":"publisher","DOI":"10.1016\/j.pain.2008.08.013"},{"key":"bibr13-1744-8069-10-40","doi-asserted-by":"publisher","DOI":"10.1016\/j.pain.2004.03.043"},{"key":"bibr14-1744-8069-10-40","doi-asserted-by":"publisher","DOI":"10.1002\/1097-4598(200101)24:1<37::AID-MUS4>3.0.CO;2-8"},{"key":"bibr15-1744-8069-10-40","doi-asserted-by":"publisher","DOI":"10.1016\/S0304-3959(03)00269-0"},{"key":"bibr16-1744-8069-10-40","doi-asserted-by":"publisher","DOI":"10.1016\/j.autneu.2011.12.004"},{"key":"bibr17-1744-8069-10-40","doi-asserted-by":"publisher","DOI":"10.1186\/1744-8069-5-1"},{"key":"bibr18-1744-8069-10-40","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuroscience.2007.10.020"},{"key":"bibr19-1744-8069-10-40","doi-asserted-by":"publisher","DOI":"10.1016\/S0896-6273(00)80564-4"},{"key":"bibr20-1744-8069-10-40","doi-asserted-by":"publisher","DOI":"10.1038\/sj.emboj.7600177"},{"key":"bibr21-1744-8069-10-40","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1108903108"},{"key":"bibr22-1744-8069-10-40","doi-asserted-by":"publisher","DOI":"10.1002\/ana.22382"},{"key":"bibr23-1744-8069-10-40","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpain.2009.08.007"},{"key":"bibr24-1744-8069-10-40","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuroscience.2009.11.029"},{"key":"bibr25-1744-8069-10-40","doi-asserted-by":"publisher","DOI":"10.1152\/jn.01067.2012"},{"key":"bibr26-1744-8069-10-40","first-page":"817347","volume":"2012","author":"Gautam M","year":"2012","journal-title":"Pain Res Treat"},{"key":"bibr27-1744-8069-10-40","doi-asserted-by":"publisher","DOI":"10.1038\/emboj.2008.213"},{"key":"bibr28-1744-8069-10-40","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.5266-10.2011"},{"key":"bibr29-1744-8069-10-40","doi-asserted-by":"publisher","DOI":"10.1111\/j.1476-5381.2010.00918.x"},{"key":"bibr30-1744-8069-10-40","doi-asserted-by":"publisher","DOI":"10.1016\/j.pharmthera.2011.12.009"},{"key":"bibr31-1744-8069-10-40","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0065751"},{"key":"bibr32-1744-8069-10-40","doi-asserted-by":"publisher","DOI":"10.1016\/j.pain.2012.04.034"},{"key":"bibr33-1744-8069-10-40","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbadis.2007.01.008"},{"key":"bibr34-1744-8069-10-40","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.0496-07.2007"},{"key":"bibr35-1744-8069-10-40","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejphar.2004.01.017"},{"key":"bibr36-1744-8069-10-40","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0611364104"},{"key":"bibr37-1744-8069-10-40","doi-asserted-by":"publisher","DOI":"10.1038\/379257a0"},{"key":"bibr38-1744-8069-10-40","doi-asserted-by":"publisher","DOI":"10.1093\/brain\/aws225"},{"key":"bibr39-1744-8069-10-40","doi-asserted-by":"publisher","DOI":"10.1111\/j.1476-5381.2011.01674.x"},{"key":"bibr40-1744-8069-10-40","doi-asserted-by":"publisher","DOI":"10.1016\/j.mcn.2007.06.010"},{"key":"bibr41-1744-8069-10-40","doi-asserted-by":"publisher","DOI":"10.1007\/s12031-013-0070-0"},{"key":"bibr42-1744-8069-10-40","doi-asserted-by":"publisher","DOI":"10.5313\/wja.v3.i1.71"},{"key":"bibr43-1744-8069-10-40","doi-asserted-by":"publisher","DOI":"10.1111\/jcmm.12025"},{"key":"bibr44-1744-8069-10-40","doi-asserted-by":"publisher","DOI":"10.1016\/j.pharmthera.2010.08.006"},{"key":"bibr45-1744-8069-10-40","doi-asserted-by":"publisher","DOI":"10.1096\/fj.12-220400"},{"key":"bibr46-1744-8069-10-40","doi-asserted-by":"publisher","DOI":"10.1186\/1744-8069-10-30"},{"key":"bibr47-1744-8069-10-40","doi-asserted-by":"publisher","DOI":"10.1152\/jn.00576.2012"},{"key":"bibr48-1744-8069-10-40","doi-asserted-by":"publisher","DOI":"10.1016\/j.expneurol.2011.12.019"},{"key":"bibr49-1744-8069-10-40","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.23-13-05437.2003"},{"key":"bibr50-1744-8069-10-40","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.1041-10.2010"},{"key":"bibr51-1744-8069-10-40","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.5564-10.2011"},{"key":"bibr52-1744-8069-10-40","doi-asserted-by":"publisher","DOI":"10.2174\/138920111798357258"},{"key":"bibr53-1744-8069-10-40","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.122245999"},{"key":"bibr54-1744-8069-10-40","doi-asserted-by":"publisher","DOI":"10.1016\/j.pain.2004.08.015"},{"key":"bibr55-1744-8069-10-40","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.0000227505.19472.69"},{"key":"bibr56-1744-8069-10-40","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2003.058693"}],"container-title":["Molecular Pain"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1186\/1744-8069-10-40","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/full-xml\/10.1186\/1744-8069-10-40","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1186\/1744-8069-10-40","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,3,10]],"date-time":"2025-03-10T20:32:53Z","timestamp":1741638773000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1186\/1744-8069-10-40"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,1,1]]},"references-count":56,"alternative-id":["10.1186\/1744-8069-10-40"],"URL":"https:\/\/doi.org\/10.1186\/1744-8069-10-40","relation":{},"ISSN":["1744-8069","1744-8069"],"issn-type":[{"value":"1744-8069","type":"print"},{"value":"1744-8069","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,1,1]]},"article-number":"1744-8069-10-40"}}