{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,8]],"date-time":"2026-06-08T11:22:15Z","timestamp":1780917735466,"version":"3.54.1"},"reference-count":55,"publisher":"SAGE Publications","license":[{"start":{"date-parts":[[2010,1,1]],"date-time":"2010-01-01T00:00:00Z","timestamp":1262304000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Mol Pain"],"published-print":{"date-parts":[[2010,1,1]]},"abstract":"<jats:sec><jats:title>Background:<\/jats:title><jats:p>Hydrogen sulphide (H<jats:sub>2<\/jats:sub>S) is a gaseous neuro-mediator that exerts analgesic effects in rodent models of visceral pain by activating K<jats:sub>ATP<\/jats:sub>channels. A body of evidence support the notion that K<jats:sub>ATP<\/jats:sub>channels interact with endogenous opioids. Whether H<jats:sub>2<\/jats:sub>S-induced analgesia involves opioid receptors is unknown.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods:<\/jats:title><jats:p>The perception of painful sensation induced by colorectal distension (CRD) in conscious rats was measured by assessing the abdominal withdrawal reflex. The contribution of opioid receptors to H<jats:sub>2<\/jats:sub>S-induced analgesia was investigated by administering rats with selective \u03bc, \u03ba and \u03b4 opioid receptor antagonists and antisenses. To investigate whether H<jats:sub>2<\/jats:sub>S causes \u03bc opioid receptor (MOR) transactivation, the neuronal like cells SKNMCs were challenged with H<jats:sub>2<\/jats:sub>S in the presence of MOR agonist (DAMGO) or antagonist (CTAP). MOR activation and phosphorylation, its association to \u03b2 arrestin and internalization were measured.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results:<\/jats:title><jats:p>H<jats:sub>2<\/jats:sub>S exerted a potent analgesic effects on CRD-induced pain. H<jats:sub>2<\/jats:sub>S-induced analgesia required the activation of the opioid system. By pharmacological and molecular analyses, a robust inhibition of H<jats:sub>2<\/jats:sub>S-induced analgesia was observed in response to central administration of CTAP and MOR antisense, while \u03ba and \u03b4 receptors were less involved. H<jats:sub>2<\/jats:sub>S caused MOR transactivation and internalization in SKNMCs by a mechanism that required AKT phosphorylation. MOR transactivation was inhibited by LY294002, a PI3K inhibitor, and glibenclamide, a K<jats:sub>ATP<\/jats:sub>channels blocker.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions:<\/jats:title><jats:p>This study provides pharmacological and molecular evidence that antinociception exerted by H<jats:sub>2<\/jats:sub>S in a rodent model of visceral pain is modulated by the transactivation of MOR. This observation provides support for development of new pharmacological approaches to visceral pain.<\/jats:p><\/jats:sec>","DOI":"10.1186\/1744-8069-6-36","type":"journal-article","created":{"date-parts":[[2010,6,11]],"date-time":"2010-06-11T18:21:12Z","timestamp":1276280472000},"source":"Crossref","is-referenced-by-count":40,"title":["Hydrogen Sulphide Induces \u03bc Opioid Receptor-Dependent Analgesia in a Rodent Model of Visceral Pain"],"prefix":"10.1177","volume":"6","author":[{"given":"Eleonora","family":"Distrutti","sequence":"first","affiliation":[{"name":"S.C. di Gastroenterologia, Azienda Ospedaliera di Perugia, Perugia, Italia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sabrina","family":"Cipriani","sequence":"additional","affiliation":[{"name":"Dipartimento di Medicina Clinica e Sperimentale, Universit\u00e0 degli Studi di Perugia, Perugia, 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