{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,29]],"date-time":"2026-10-29T04:24:56Z","timestamp":1793247896942,"version":"4.5.0"},"reference-count":75,"publisher":"SAGE Publications","license":[{"start":{"date-parts":[[2011,1,1]],"date-time":"2011-01-01T00:00:00Z","timestamp":1293840000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Mol Pain"],"published-print":{"date-parts":[[2011,1,1]]},"abstract":"<jats:sec><jats:title>Background:<\/jats:title><jats:p> Lentivector-mediated gene delivery into the dorsal root ganglion (DRG) is a promising method for exploring pain pathophysiology and for genetic treatment of chronic neuropathic pain. In this study, a series of modified lentivector particles with different cellular promoters, envelope glycoproteins, and viral accessory proteins were generated to evaluate the requirements for efficient transduction into neuronal cells in vitro and adult rat DRG in vivo. <\/jats:p><\/jats:sec><jats:sec><jats:title>Results:<\/jats:title><jats:p> In vitro, lentivectors expressing enhanced green fluorescent protein (EGFP) under control of the human elongation factor 1\u03b1 (EF1\u03b1) promoter and pseudotyped with the conventional vesicular stomatitis virus G protein (VSV-G) envelope exhibited the best performance in the transfer of EGFP into an immortalized DRG sensory neuron cell line at low multiplicities of infection (MOIs), and into primary cultured DRG neurons at higher MOIs. In vivo, injection of either first or second-generation EF1\u03b1-EGFP lentivectors directly into adult rat DRGs led to transduction rates of 19 \u00b1 9% and 20 \u00b1 8% EGFP-positive DRG neurons, respectively, detected at 4 weeks post injection. Transduced cells included a full range of neuronal phenotypes, including myelinated neurons as well as both non-peptidergic and peptidergic nociceptive unmyelinated neurons. <\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion:<\/jats:title><jats:p> VSV-G pseudotyped lentivectors containing the human elongation factor 1\u03b1 (EF1\u03b1)-EGFP expression cassette demonstrated relatively efficient transduction to sensory neurons following direct injection into the DRG. These results clearly show the potential of lentivectors as a viable system for delivering target genes into DRGs to explore basic mechanisms of neuropathic pain, with the potential for future clinical use in treating chronic pain. <\/jats:p><\/jats:sec>","DOI":"10.1186\/1744-8069-7-63","type":"journal-article","created":{"date-parts":[[2011,8,23]],"date-time":"2011-08-23T20:37:14Z","timestamp":1314131834000},"update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":45,"title":["Lentiviral Gene Transfer into the Dorsal Root Ganglion of Adult Rats"],"prefix":"10.1177","volume":"7","author":[{"given":"gwei","family":"Yu","sequence":"first","affiliation":[{"name":"Department of Anesthesiology, Medical College of Wisconsin, 8100 Watertown Plank Rd, Milwaukee, Wisconsin, 53226, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Greg","family":"Fischer","sequence":"additional","affiliation":[{"name":"Department of Anesthesiology, Medical College of Wisconsin, 8100 Watertown Plank Rd, Milwaukee, Wisconsin, 53226, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guangfu","family":"Jia","sequence":"additional","affiliation":[{"name":"Department of Medicine, Medical College of Wisconsin, 8100 Watertown Plank Rd, Milwaukee, Wisconsin, 53226, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jakob","family":"Reiser","sequence":"additional","affiliation":[{"name":"U.S. Food and Drug Administration, Center for Biologics Evaluation and Research, Division of Cellular and Gene Therapies, 1401 Rockville Pike Suite 200N\/HFM-47 Rockville, Maryland, 20852-1448, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Frank","family":"Park","sequence":"additional","affiliation":[{"name":"Department of Medicine, Medical College of Wisconsin, 8100 Watertown Plank Rd, Milwaukee, Wisconsin, 53226, USA"},{"name":"Department of Physiology, Medical College of Wisconsin, 8100 Watertown Plank Rd, Milwaukee, Wisconsin, 53226, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Quinn H","family":"Hogan","sequence":"additional","affiliation":[{"name":"Department of Anesthesiology, Medical College of Wisconsin, 8100 Watertown Plank Rd, Milwaukee, Wisconsin, 53226, USA"},{"name":"Milwaukee VA Medical Center, 5000 West National Ave, Milwaukee, Wisconsin, 53226, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2011,1,1]]},"reference":[{"key":"bibr1-1744-8069-7-63","doi-asserted-by":"publisher","DOI":"10.1634\/theoncologist.2009-S502"},{"key":"bibr2-1744-8069-7-63","first-page":"129","volume-title":"Textbook of Pain.","author":"Devor M","year":"1999","edition":"4"},{"key":"bibr3-1744-8069-7-63","doi-asserted-by":"publisher","DOI":"10.1016\/S0301-0082(98)00007-0"},{"key":"bibr4-1744-8069-7-63","doi-asserted-by":"publisher","DOI":"10.1038\/gt.2009.23"},{"key":"bibr5-1744-8069-7-63","doi-asserted-by":"publisher","DOI":"10.1038\/mt.2008.213"},{"key":"bibr6-1744-8069-7-63","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0708003105"},{"key":"bibr7-1744-8069-7-63","doi-asserted-by":"crossref","unstructured":"Huang X, Yang Y: Innate Immune Recognition of Viruses and Viral Vectors. 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