{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T23:44:13Z","timestamp":1792712653497,"version":"4.5.0"},"reference-count":50,"publisher":"SAGE Publications","license":[{"start":{"date-parts":[[2012,1,1]],"date-time":"2012-01-01T00:00:00Z","timestamp":1325376000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Mol Pain"],"published-print":{"date-parts":[[2012,1,1]]},"abstract":"<jats:sec><jats:title>Background:<\/jats:title><jats:p>It has been reported that the P2Y<jats:sub>12<\/jats:sub>receptor (P2Y<jats:sub>12<\/jats:sub>R) is involved in satellite glial cells (SGCs) activation, indicating that P2Y<jats:sub>12<\/jats:sub>R expressed in SGCs may play functional roles in orofacial neuropathic pain mechanisms. However, the involvement of P2Y<jats:sub>12<\/jats:sub>R in orofacial neuropathic pain mechanisms is still unknown. We therefore studied the reflex to noxious mechanical or heat stimulation of the tongue, P2Y<jats:sub>12<\/jats:sub>R and glial fibrillary acidic protein (GFAP) immunohistochemistries in the trigeminal ganglion (TG) in a rat model of unilateral lingual nerve crush (LNC) to evaluate role of P2Y<jats:sub>12<\/jats:sub>R in SGC in lingual neuropathic pain.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results:<\/jats:title><jats:p>The head-withdrawal reflex thresholds to mechanical and heat stimulation of the lateral tongue were significantly decreased in LNC-rats compared to sham-rats. These nocifensive effects were apparent on day 1 after LNC and lasted for 17 days. On days 3, 9, 15 and 21 after LNC, the mean relative number of TG neurons encircled with GFAP-immunoreactive (IR) cells significantly increased in the ophthalmic, maxillary and mandibular branch regions of TG. On day 3 after LNC, P2Y<jats:sub>12<\/jats:sub>R expression occurred in GFAP-IR cells but not neuronal nuclei (NeuN)-IR cells (i.e. neurons) in TG. After 3 days of successive administration of the P2Y<jats:sub>12<\/jats:sub>R antagonist MRS2395 into TG in LNC-rats, the mean relative number of TG neurons encircled with GFAP-IR cells was significantly decreased coincident with a significant reversal of the lowered head-withdrawal reflex thresholds to mechanical and heat stimulation of the tongue compared to vehicle-injected rats. Furthermore, after 3 days of successive administration of the P2YR agonist 2-MeSADP into the TG in na\u00efve rats, the mean relative number of TG neurons encircled with GFAP-IR cells was significantly increased and head-withdrawal reflex thresholds to mechanical and heat stimulation of the tongue were significantly decreased in a dose-dependent manner compared to vehicle-injected rats.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions:<\/jats:title><jats:p>The present findings provide the first evidence that the activation of P2Y<jats:sub>12<\/jats:sub>R in SGCs of TG following lingual nerve injury is involved in the enhancement of TG neuron activity and nocifensive reflex behavior, resulting in neuropathic pain in the tongue.<\/jats:p><\/jats:sec>","DOI":"10.1186\/1744-8069-8-23","type":"journal-article","created":{"date-parts":[[2012,3,30]],"date-time":"2012-03-30T02:14:20Z","timestamp":1333073660000},"update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":107,"title":["Satellite Glial Cell P2Y<sub>12<\/sub>Receptor in the Trigeminal Ganglion is Involved in Lingual Neuropathic Pain Mechanisms in Rats"],"prefix":"10.1177","volume":"8","author":[{"given":"Ayano","family":"Katagiri","sequence":"first","affiliation":[{"name":"Department of Physiology, Nihon University School of Dentistry, 1-8-13 Kandasurugadai, Chiyoda-ku, Tokyo 101-8310, Japan"},{"name":"Department of Psychosomatic Dentistry, Tokyo Medical and Dental University Graduate School, 1-5-45 Yushima, Bunkyo-ku, Tokyo 113-8549, 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Psychosomatic Dentistry, Tokyo Medical and Dental University Graduate School, 1-5-45 Yushima, Bunkyo-ku, Tokyo 113-8549, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Barry J","family":"Sessle","sequence":"additional","affiliation":[{"name":"Department of Oral Physiology, Faculty of Dentistry, University of Toronto, 124 Edward Street, Toronto, Ontario M5G 1G6, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Koichi","family":"Iwata","sequence":"additional","affiliation":[{"name":"Department of Physiology, Nihon University School of Dentistry, 1-8-13 Kandasurugadai, Chiyoda-ku, Tokyo 101-8310, Japan"},{"name":"Division of Functional Morphology, Dental Research Center, Nihon University School of Dentistry, 1-8-13 Kandasurugadai, Chiyoda-ku, Tokyo 101-8310, Japan"},{"name":"Division of Applied System Neuroscience Advanced Medical Research Center, Nihon University Graduate School of Medical Science, 30-1 Ohyaguchi-Kamimachi, Itabashi, Tokyo 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