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Light chain restriction (LCR) was detected in the 32 FCI diagnosed B-NHL. The overall concordance between FCI versus histopathology and IHC was 88%. The sensitivity and specificity of FCI in diagnosis of NHL was 94.9% and 100% respectively; in HL they were 40% and 100% respectively and in NLT, both sensitivity and specificity were 100% while for RH were 100% and 89.1% respectively.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>FCI is a sensitive and specific method in diagnosis and classification of NHL as well as in detection of monoclonality. False negative results could be due to the presence of heterogeneous populations of lymphocytes in special types of lymphoma.<\/jats:p><\/jats:sec>","DOI":"10.1186\/1746-1596-3-43","type":"journal-article","created":{"date-parts":[[2008,11,6]],"date-time":"2008-11-06T19:13:31Z","timestamp":1225998811000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":21,"title":["Flow cytometric immunophenotyping (FCI) of lymphoma: correlation with histopathology and immunohistochemistry"],"prefix":"10.1186","volume":"3","author":[{"given":"Abeer M","family":"El-Sayed","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mohammad H","family":"El-Borai","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Abeer A","family":"Bahnassy","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shadia MS","family":"El-Gerzawi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2008,11,6]]},"reference":[{"issue":"5","key":"165_CR1","doi-asserted-by":"crossref","first-page":"1035","DOI":"10.26719\/1999.5.5.1035","volume":"5","author":"JA Al-Lawati","year":"1999","unstructured":"Al-Lawati JA, Santhosh-Kumar CR, Mohammed AJ, Jaffer MA: Cancer incidence in Oman, 1993\u20131997. 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