{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,23]],"date-time":"2026-02-23T13:05:36Z","timestamp":1771851936403,"version":"3.50.1"},"reference-count":30,"publisher":"Springer Science and Business Media LLC","issue":"1","content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["BMC Vet Res"],"published-print":{"date-parts":[[2006,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n          <jats:sec>\n            <jats:title>Background<\/jats:title>\n            <jats:p>We compared skin biopsy samples from different anatomical regions for detecting <jats:italic>Leishmania<\/jats:italic> in dogs, using histological (HE), immunohistochemical (IHC) and polymerase chain reaction (PCR) techniques.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Results<\/jats:title>\n            <jats:p>The sensitivity was 82.8 percent for PCR, 62.1 percent for IHC and 44.8 percent for HE. These methods do not appear to depend on the clinical status of the animal or the anatomical source of the skin sample; there is no \"best region\" for any method. However, PCR was more effective than IHC and HE for ear and nose skin samples whereas IHC was better than HE for nose samples. There was weak agreement between PCR and HE for all tissue samples; good agreement between PCR and IHC for ear and abdomen samples, and weak agreement for nose; and optimal agreement between IHC and HE for ear and abdomen and good agreement for nose samples.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Conclusion<\/jats:title>\n            <jats:p>The PCR on ear skin could be the best procedure for diagnosing canine visceral leishmaniasis. The good agreement between PCR and IHC indicates that IHC can be used as an alternative method. Finally, tissue samples from ears, nose and abdomen, particularly ears and nose, are potentially useful for diagnosing canine visceral leishmaniasis independently of the animal's clinical status.<\/jats:p>\n          <\/jats:sec>","DOI":"10.1186\/1746-6148-2-17","type":"journal-article","created":{"date-parts":[[2006,6,8]],"date-time":"2006-06-08T18:32:10Z","timestamp":1149791530000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":54,"title":["Comparison of paraffin-embedded skin biopsies from different anatomical regions as sampling methods for detection of Leishmaniainfection in dogs using histological, immunohistochemical and PCR methods"],"prefix":"10.1186","volume":"2","author":[{"given":"S\u00edlvio Coura","family":"Xavier","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H\u00e9lida Monteiro","family":"de Andrade","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sem\u00edramis Jamil Hadad","family":"Monte","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ingrid Maria","family":"Chiarelli","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wanderson Geraldo","family":"Lima","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marilene Suzan Marques","family":"Michalick","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Washington Luiz","family":"Tafuri","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wagner Luiz","family":"Tafuri","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2006,6,8]]},"reference":[{"key":"26_CR1","doi-asserted-by":"publisher","first-page":"557","DOI":"10.2307\/3283159","volume":"77","author":"P Abranches","year":"1991","unstructured":"Abranches P, Silva MC-Pereira, Conceicao-Silva FM, Santos-Gomes GM, Janz JG: Canine leishmaniasis: pathological and ecological factors influencing transmission of infection. 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