{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,9]],"date-time":"2026-04-09T13:44:24Z","timestamp":1775742264223,"version":"3.50.1"},"reference-count":36,"publisher":"Springer Science and Business Media LLC","issue":"1","content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["BMC Vet Res"],"published-print":{"date-parts":[[2008,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n          <jats:sec>\n            <jats:title>Background<\/jats:title>\n            <jats:p>Results regarding the use of bovine somatotropin for enhancing fertility in dairy cattle are variable. Here, the hypothesis was tested that a single injection of a sustained-release preparation of bovine somatotropin (bST) during the preovulatory period would improve pregnancy success of lactating dairy cows at first service.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Results<\/jats:title>\n            <jats:p>The first experiment was conducted in a temperate region of Mexico. Cows inseminated following natural estrus or timed artificial insemination were given a single injection of bST or a placebo injection at insemination (n = 100 cows per group). There was no significant difference between bST and control groups in the proportion of inseminated cows diagnosed pregnant (29 vs 31% pregnant). The second experiment was performed during heat stress in Florida. Cows were subjected to an ovulation synchronization regimen for first insemination. Cows treated with bST received a single injection at 3 days before insemination. Controls received no additional treatment. As expected, bST did not increase vaginal temperature. Treatment with bST did not significantly increase the proportion of inseminated cows diagnosed pregnant although it was numerically greater for the bST group (24.2% vs 17.8%, 124\u2013132 cows per group). There was a tendency (<jats:italic>p<\/jats:italic> = 0.10) for a smaller percent of control cows to have high plasma progesterone concentrations (\u2265 1 ng\/ml) at Day 7 after insemination than for bST-treated cows (72.6 vs 81.1%). When only cows that were successfully synchronized were considered, the magnitude of the absolute difference in the percentage of inseminated cows that were diagnosed pregnant between bST and control cows was reduced (24.8 vs 22.4% pregnant for bST and control).<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Conclusion<\/jats:title>\n            <jats:p>Results failed to indicate a beneficial effect of bST treatment on fertility of lactating dairy cows.<\/jats:p>\n          <\/jats:sec>","DOI":"10.1186\/1746-6148-4-22","type":"journal-article","created":{"date-parts":[[2008,6,27]],"date-time":"2008-06-27T06:13:20Z","timestamp":1214547200000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Pregnancy success of lactating Holstein cows after a single administration of a sustained-release formulation of recombinant bovine somatotropin"],"prefix":"10.1186","volume":"4","author":[{"given":"A","family":"Bell","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"OA","family":"Rodr\u00edguez","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"LA","family":"de Castro e Paula","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"MB","family":"Padua","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J","family":"Hern\u00e1ndez-Cer\u00f3n","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"CG","family":"Guti\u00e9rrez","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A","family":"De Vries","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"PJ","family":"Hansen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2008,6,26]]},"reference":[{"key":"103_CR1","doi-asserted-by":"publisher","first-page":"355","DOI":"10.3168\/jds.S0022-0302(88)79564-8","volume":"71","author":"CG Soderholm","year":"1988","unstructured":"Soderholm CG, Otterby DE, Linn JG, Ehle FR, Wheaton JE, Hansen WP, Annexstad RJ: Effects of recombinant bovine somatotropin on milk production, body composition, and physiological parameters. J Dairy Sci. 1988, 71: 355-365.","journal-title":"J Dairy Sci"},{"key":"103_CR2","doi-asserted-by":"publisher","first-page":"907","DOI":"10.3168\/jds.S0022-0302(89)79184-0","volume":"72","author":"A Zoa-Mboe","year":"1989","unstructured":"Zoa-Mboe A, Head HH, Bachman KC, Baccari F, Wilcox CJ: Effects of bovine somatotropin on milk yield and composition, dry matter intake, and some physiological functions of Holstein dairy cows during heat stress. J Dairy Sci. 1989, 72: 907-916.","journal-title":"J Dairy Sci"},{"key":"103_CR3","doi-asserted-by":"publisher","first-page":"2896","DOI":"10.3168\/jds.S0022-0302(90)78977-1","volume":"73","author":"JW West","year":"1990","unstructured":"West JW, Mullinix BG, Johnson JC, Ash KA, Taylor VN: Effects of bovine somatotropin on dry matter intake, milk yield, and body temperature in Holstein and Jersey cows during heat stress. J Dairy Sci. 1990, 73: 2896-2906.","journal-title":"J Dairy Sci"},{"key":"103_CR4","doi-asserted-by":"publisher","first-page":"3402","DOI":"10.3168\/jds.S0022-0302(92)78116-8","volume":"75","author":"BF Jenny","year":"1992","unstructured":"Jenny BF, Grimes LW, Pardue FE, Rock DW, Patterson DL: Lactational response of Jersey cows to bovine somatotropin administered daily or in a sustained-release formulation. J Dairy Sci. 1992, 75: 3402-3407.","journal-title":"J Dairy Sci"},{"key":"103_CR5","doi-asserted-by":"publisher","first-page":"449","DOI":"10.3168\/jds.S0022-0302(92)77781-9","volume":"75","author":"F Elvinger","year":"1992","unstructured":"Elvinger F, Natzke RP, Hansen PJ: Interactions of heat stress and bovine somatotropin affecting physiology and immunology of lactating cows. J Dairy Sci. 1992, 75: 449-462.","journal-title":"J Dairy Sci"},{"key":"103_CR6","doi-asserted-by":"publisher","first-page":"341","DOI":"10.3168\/jds.S0022-0302(07)72635-8","volume":"90","author":"FD Jousan","year":"2007","unstructured":"Jousan FD, de Castro e Paula LA, Block J, Hansen PJ: Fertility of lactating dairy cows administered recombinant bovine somatotropin during heat stress. J Dairy Sci. 2007, 90: 341-351.","journal-title":"J Dairy Sci"},{"key":"103_CR7","doi-asserted-by":"publisher","first-page":"2549","DOI":"10.3168\/jds.S0022-0302(94)77196-4","volume":"77","author":"E Esteban","year":"1994","unstructured":"Esteban E, Kass PH, Weaver LD, Rowe JD, Holmberg CA, Franti CE, Troutt HF: Interval from calving to conception in high producting dairy cows treated with recombinant bovine somatotropin. J Dairy Sci. 1994, 77: 2549-2561.","journal-title":"J Dairy Sci"},{"key":"103_CR8","doi-asserted-by":"publisher","first-page":"3371","DOI":"10.3168\/jds.S0022-0302(94)77279-9","volume":"77","author":"E Esteban","year":"1994","unstructured":"Esteban E, Kass PH, Weaver LD, Rowe JD, Holmberg CA, Franti CE, Troutt HF: Reproductive performance in high producing dairy cows treated with recombinant bovine somatotropin. J Dairy Sci. 1994, 77: 3371-3381.","journal-title":"J Dairy Sci"},{"key":"103_CR9","doi-asserted-by":"publisher","first-page":"1237","DOI":"10.3168\/jds.S0022-0302(00)74990-3","volume":"83","author":"F Moreira","year":"2000","unstructured":"Moreira F, Risco CA, Pires MFA, Ambrose JD, Drost M, Thatcher WW: Use of bovine somatotropin in lactating dairy cows receiving timed artificial insemination. J Dairy Sci. 2000, 83: 1237-1247.","journal-title":"J Dairy Sci"},{"key":"103_CR10","doi-asserted-by":"publisher","first-page":"1646","DOI":"10.3168\/jds.S0022-0302(01)74600-0","volume":"84","author":"F Moreira","year":"2001","unstructured":"Moreira F, Orlandi C, Risco CA, Mattos R, Lopes F, Thatcher WW: Effects of presynchronization and bovine somatotropin on pregnancy rates to a timed artificial insemination protocol in lactating dairy cows. J Dairy Sci. 2001, 84: 1646-1659.","journal-title":"J Dairy Sci"},{"key":"103_CR11","doi-asserted-by":"publisher","first-page":"868","DOI":"10.3168\/jds.S0022-0302(04)73231-2","volume":"87","author":"JEP Santos","year":"2004","unstructured":"Santos JEP, Juchem SO, Cerri RLA, Galvao KN, Chebel RC, Thatcher WW, Dei CS, Bilby CR: Effect of bST and reproductive management on reproductive performance of Holstein dairy cows. J Dairy Sci. 2004, 87: 868-881.","journal-title":"J Dairy Sci"},{"key":"103_CR12","doi-asserted-by":"publisher","first-page":"4176","DOI":"10.3168\/jds.S0022-0302(06)72463-8","volume":"89","author":"CA Blevins","year":"2006","unstructured":"Blevins CA, Shirley JE, Stevenson JS: Milking frequency, estradiol cypionate, and somatotropin influence lactation and reproduction in dairy cows. J Dairy Sci. 2006, 89: 4176-4187.","journal-title":"J Dairy Sci"},{"key":"103_CR13","doi-asserted-by":"publisher","first-page":"1831","DOI":"10.1016\/S0093-691X(01)00525-8","volume":"55","author":"JS Morales-Roura","year":"2001","unstructured":"Morales-Roura JS, Zarco L, Hern\u00e1ndez-C\u00e9ron J, Rodr\u00edguez G: Effect of short term treatment with bovine somatotropin at estrus on conception rate and luteal function of repeat breeding dairy cows. Theriogenology. 2001, 55: 1831-1841. 10.1016\/S0093-691X(01)00525-8.","journal-title":"Theriogenology"},{"key":"103_CR14","doi-asserted-by":"crossref","first-page":"2901","DOI":"10.2527\/1992.7092901x","volume":"70","author":"MA McGuire","year":"1992","unstructured":"McGuire MA, Vicini JL, Bauman DE, Veenhuizen JJ: Insulin-like growth factors and binding proteins in ruminants and their nutritional regulation. J Anim Sci. 1992, 70: 2901-2910.","journal-title":"J Anim Sci"},{"key":"103_CR15","doi-asserted-by":"publisher","first-page":"1665","DOI":"10.1095\/biolreprod.104.032102","volume":"71","author":"FD Jousan","year":"2004","unstructured":"Jousan FD, Hansen PJ: Insulin-like growth factor-I as a survival factor for the bovine preimplantation embryo exposed to heat shock. Biol Reprod. 2004, 71: 1665-1670. 10.1095\/biolreprod.104.032102.","journal-title":"Biol Reprod"},{"key":"103_CR16","doi-asserted-by":"publisher","first-page":"189","DOI":"10.1002\/mrd.20527","volume":"74","author":"FD Jousan","year":"2007","unstructured":"Jousan FD, Hansen PJ: Insulin-like growth factor-I promotes resistance of bovine preimplantation embryos to heat shock through actions independent of its anti-apoptotic actions requiring PI3K signaling. Mol Reprod Dev. 2007, 74: 189-196. 10.1002\/mrd.20527.","journal-title":"Mol Reprod Dev"},{"key":"103_CR17","doi-asserted-by":"crossref","first-page":"1590","DOI":"10.2527\/2003.8161590x","volume":"81","author":"J Block","year":"2003","unstructured":"Block J, Drost M, Monson RL, Rutledge JJ, Rivera RM, Paula-Lopes FF, Ocon OM, Krininger CE, Liu J, Hansen PJ: Use of insulin-like growth factor-I during embryo culture and treatment of recipients with gonadotropin-releasing hormone to increase pregnancy rates following the transfer of in vitro-produced embryos to heat-stressed, lactating dairy cows. J Anim Sci. 2003, 81: 1590-1602.","journal-title":"J Anim Sci"},{"key":"103_CR18","doi-asserted-by":"publisher","first-page":"1518","DOI":"10.1016\/j.theriogenology.2007.03.012","volume":"67","author":"J Block","year":"2007","unstructured":"Block J, Hansen PJ: Interaction between season and culture with insulin-like growth factor-I on survival of in-vitro produced embryos following transfer to lactating dairy cows. Theriogenology. 2007, 67: 1518-1529. 10.1016\/j.theriogenology.2007.03.012.","journal-title":"Theriogenology"},{"key":"103_CR19","doi-asserted-by":"publisher","first-page":"1635","DOI":"10.3168\/jds.S0022-0302(00)75032-6","volume":"83","author":"MC Lucy","year":"2000","unstructured":"Lucy MC: Regulation of ovarian follicular growth by somatotropin and insulin-like growth factors in cattle. J Dairy Sci. 2000, 83: 1635-1647.","journal-title":"J Dairy Sci"},{"key":"103_CR20","doi-asserted-by":"crossref","first-page":"E40","DOI":"10.2527\/2004.8213_supplE40x","volume":"82","author":"SM Quirk","year":"2004","unstructured":"Quirk SM, Cowan RG, Harman RM, Hu CL, Porter DA: Ovarian follicular growth and atresia: the relationship between cell proliferation and survival. J Anim Sci. 2004, 82: E40-52.","journal-title":"J Anim Sci"},{"key":"103_CR21","doi-asserted-by":"publisher","first-page":"179","DOI":"10.1016\/j.domaniend.2004.03.003","volume":"27","author":"F Nuttinck","year":"2004","unstructured":"Nuttinck F, Charpigny G, Mermillod P, Loosfelt H, Meduri G, Freret S, Grimard B, Heyman Y: Expression of components of the insulin-like growth factor system and gonadotropin receptors in bovine cumulus-oocyte complexes during oocyte maturation. Domest Anim Endocrinol. 2004, 27: 179-95. 10.1016\/j.domaniend.2004.03.003.","journal-title":"Domest Anim Endocrinol"},{"key":"103_CR22","doi-asserted-by":"publisher","first-page":"419","DOI":"10.1262\/jrd.18076","volume":"53","author":"M Wasielak","year":"2007","unstructured":"Wasielak M, Bogacki M: Apoptosis inhibition by insulin-like growth factor (IGF)-I during in vitro maturation of bovine oocytes. J Reprod Dev. 2007, 53: 419-426. 10.1262\/jrd.18076.","journal-title":"J Reprod Dev"},{"key":"103_CR23","doi-asserted-by":"publisher","first-page":"895","DOI":"10.1016\/S0093-691X(01)00694-X","volume":"57","author":"F Moreira","year":"2002","unstructured":"Moreira F, Paula-Lopes FF, Hansen PJ, Badinga L, Thatcher WW: Effects of growth hormone and insulin-like growth factor-I on development of in vitro derived bovine embryos. Theriogenology. 2002, 57: 895-907. 10.1016\/S0093-691X(01)00694-X.","journal-title":"Theriogenology"},{"key":"103_CR24","doi-asserted-by":"publisher","first-page":"101","DOI":"10.1007\/s00441-004-0898-2","volume":"317","author":"S K\u00f6lle","year":"2004","unstructured":"K\u00f6lle S, Stojkovic M, Reese S, Reichenbach HD, Wolf E, Sinowatz F: Effects of growth hormone on the ultrastructure of bovine preimplantation embryos. Cell Tissue Res. 2004, 317: 101-108. 10.1007\/s00441-004-0898-2.","journal-title":"Cell Tissue Res"},{"key":"103_CR25","doi-asserted-by":"publisher","first-page":"3601","DOI":"10.3168\/jds.S0022-0302(94)77304-5","volume":"77","author":"AD Ealy","year":"1994","unstructured":"Ealy AD, Ar\u00e9chiga CF, Bray DR, Risco CA, Hansen PJ: Effectiveness of short-term cooling and vitamin E for alleviation of infertility induced by heat stress in dairy cows. J Dairy Sci. 1994, 77: 3601-3607.","journal-title":"J Dairy Sci"},{"key":"103_CR26","doi-asserted-by":"publisher","first-page":"1285","DOI":"10.1016\/S0093-691X(99)00072-2","volume":"51","author":"CR Bilby","year":"1999","unstructured":"Bilby CR, Bader JF, Salfen BE, Youngquist RS, Murphy CN, Garverick HA, Crooker BA, Lucy MC: Plasma GH, IGF-I, and conception rate in cattle treated with low doses of recombinant bovine GH. Theriogenology. 1999, 51: 1285-1296. 10.1016\/S0093-691X(99)00072-2.","journal-title":"Theriogenology"},{"key":"103_CR27","doi-asserted-by":"publisher","first-page":"S242","DOI":"10.1016\/j.theriogenology.2007.04.008","volume":"68S","author":"PJ Hansen","year":"2007","unstructured":"Hansen PJ: Exploitation of genetic and physiological determinants of embryonic resistance to elevated temperature to improve embryonic survival in dairy cattle during heat stress. Theriogenology. 2007, 68S: S242-S249. 10.1016\/j.theriogenology.2007.04.008.","journal-title":"Theriogenology"},{"key":"103_CR28","doi-asserted-by":"publisher","first-page":"73","DOI":"10.1016\/0739-7240(94)00010-X","volume":"12","author":"MC Lucy","year":"1995","unstructured":"Lucy MC, Thatcher WW, Collier RJ, Simmen FA, Ko Y, Savio JD, Badinga L: Effects of somatotropin on the conceptus, uterus, and ovary during maternal recognition of pregnancy in cattle. Dom Anim Endocrinol. 1995, 12: 73-82. 10.1016\/0739-7240(94)00010-X.","journal-title":"Dom Anim Endocrinol"},{"key":"103_CR29","doi-asserted-by":"publisher","first-page":"437","DOI":"10.1016\/0093-691X(94)90079-X","volume":"41","author":"JC Dalton","year":"1994","unstructured":"Dalton JC, Marcinkowski DP: Effect of sometribove administration on LH concentration in dairy cattle. Theriogenology. 1994, 41: 437-445. 10.1016\/0093-691X(94)90079-X.","journal-title":"Theriogenology"},{"key":"103_CR30","doi-asserted-by":"publisher","first-page":"815","DOI":"10.1095\/biolreprod42.5.815","volume":"42","author":"SR Schemm","year":"1990","unstructured":"Schemm SR, Deaver DR, Griel LC, Mueller LD: Effects of recombinant bovine somatotropin on luteinizing hormone and ovarian function in lactating dairy cows. Biol Reprod. 1990, 42: 815-821. 10.1095\/biolreprod42.5.815.","journal-title":"Biol Reprod"},{"key":"103_CR31","unstructured":"Servicio Meteorol\u00f3gico Nacional. [http:\/\/smn.cna.gob.mx\/]"},{"key":"103_CR32","volume-title":"Nutrient Requirements of Dairy Cattle","author":"National Research Council","year":"2001","unstructured":"National Research Council: Nutrient Requirements of Dairy Cattle. National Academies Press, Washington DC, 7 Revised; 2001.","edition":"7 Revised"},{"key":"103_CR33","doi-asserted-by":"publisher","first-page":"68","DOI":"10.3168\/jds.S0022-0302(89)79081-0","volume":"72","author":"AJ Edmonson","year":"1989","unstructured":"Edmonson AJ, Lean IJ, Weaver LD, Farver T, Webster G: A body condition scoring chart for Holstein dairy cows. J Dairy Sci. 1989, 72: 68-78.","journal-title":"J Dairy Sci"},{"key":"103_CR34","doi-asserted-by":"publisher","first-page":"295","DOI":"10.3168\/jds.S0022-0302(97)75937-X","volume":"80","author":"JR Pursley","year":"1997","unstructured":"Pursley JR, Wiltbank MC, Stevenson JS, Ottobre JS, Garverick HA, Anderson LL: Pregnancy rates per artificial insemination for cows and heifers inseminated at a synchronized ovulation or synchronized estrus. J Dairy Sci. 1997, 80: 295-300.","journal-title":"J Dairy Sci"},{"key":"103_CR35","unstructured":"Florida Automated Weather Network. [http:\/\/fawn.ifas.ufl.edu]"},{"key":"103_CR36","doi-asserted-by":"publisher","first-page":"914","DOI":"10.3168\/jds.S0022-0302(05)72758-2","volume":"88","author":"MA Portaluppi","year":"2005","unstructured":"Portaluppi MA, Stevenson JS: Pregnancy rates in lactating dairy cows after presynchronization of estrous cycles and variations of the Ovsynch protocol. J Dairy Sci. 2005, 88: 914-921.","journal-title":"J Dairy Sci"}],"container-title":["BMC Veterinary Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1186\/1746-6148-4-22.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,8,31]],"date-time":"2021-08-31T22:51:16Z","timestamp":1630450276000},"score":1,"resource":{"primary":{"URL":"https:\/\/bmcvetres.biomedcentral.com\/articles\/10.1186\/1746-6148-4-22"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2008,6,26]]},"references-count":36,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2008,12]]}},"alternative-id":["103"],"URL":"https:\/\/doi.org\/10.1186\/1746-6148-4-22","relation":{},"ISSN":["1746-6148"],"issn-type":[{"value":"1746-6148","type":"electronic"}],"subject":[],"published":{"date-parts":[[2008,6,26]]},"assertion":[{"value":"9 January 2008","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"26 June 2008","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"26 June 2008","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}}],"article-number":"22"}}