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Fast and simple detection methods would be critical for assessing infection prevalence, study the mechanisms of pathogen transmission and monitoring the effects of TB control measures.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Results<\/jats:title>\n            <jats:p>An enzyme-linked immunosorbent assay (ELISA) to detect antibodies against <jats:italic>Mycobacterium bovis<\/jats:italic> in wild boar serum was developed and validated on 185 sera from TB positive and negative wild boar. Based on antigen inoculation of captive animals as well as tuberculosis compatible lesions, culture results and molecular analysis of hunted individuals, animals were allocated into two groups: tuberculosis positive group and tuberculosis negative group. After optimization of the positive to negative ratio using different combinations of serum dilutions and conjugate concentrations, the test yielded a sensitivity of 72.60% and a specificity of 96.43% for the best cut-off.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Conclusion<\/jats:title>\n            <jats:p>Although some negative group animals showed an ELISA positive reaction (&lt; 3%), this assay showed a high potential for accurate diagnosis of TB in wild boar, as its large dynamic range supported a good discriminatory power and a satisfactory balance between sensitivity and specificity.<\/jats:p>\n          <\/jats:sec>","DOI":"10.1186\/1746-6148-4-43","type":"journal-article","created":{"date-parts":[[2008,11,1]],"date-time":"2008-11-01T07:13:25Z","timestamp":1225523605000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":49,"title":["Development and validation of an enzyme-linked immunosorbent assay for antibodies against Mycobacterium bovisin european wild boar"],"prefix":"10.1186","volume":"4","author":[{"given":"Olaia","family":"Aurtenetxe","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marta","family":"Barral","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joaqu\u00edn","family":"Vicente","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jos\u00e9","family":"de la Fuente","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christian","family":"Gort\u00e1zar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ram\u00f3n A","family":"Juste","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2008,11,1]]},"reference":[{"key":"124_CR1","doi-asserted-by":"crossref","first-page":"317","DOI":"10.20506\/rst.21.2.1339","volume":"21","author":"GW de Lisle","year":"2002","unstructured":"de Lisle GW, Bengis RG, Schmitt SM, O'Brien DJ: Tuberculosis in free-ranging wildlife: detection, diagnosis and management. 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