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The aim of this study was to determine the prevalence and strain diversity of <jats:italic>L. monocytogenes<\/jats:italic> in healthy ruminants and swine herds.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Results<\/jats:title>\n            <jats:p>Faecal samples from 30 animals per herd were collected from 343 herds (120 sheep, 124 beef cattle, 82 dairy cattle and 17 swine) in the Basque Country and screened in pools by an automated enzyme-linked fluorescent immunoassay (VIDAS<jats:sup>\u00ae<\/jats:sup>) to estimate the prevalence of positive herds. Positive samples were subcultured onto the selective and differential agar ALOA and biochemically confirmed. <jats:italic>L. monocytogenes<\/jats:italic> was isolated from 46.3% of dairy cattle, 30.6% beef cattle and 14.2% sheep herds, but not from swine. Within-herd prevalence investigated by individually analysing 197 sheep and 221 cattle detected 1.5% of faecal shedders in sheep and 21.3% in cattle. Serotyping of 114 isolates identified complex 4b as the most prevalent (84.2%), followed by 1\/2a (13.2%), and PFGE analysis of 68 isolates showed a highly diverse <jats:italic>L. monocytogenes<\/jats:italic> population in ruminant herds.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Conclusion<\/jats:title>\n            <jats:p>These results suggested that cattle represent a potentially important reservoir for <jats:italic>L. monocytogenes<\/jats:italic> in the Basque Country, and highlighted the complexity of pathogen control at the farm level.<\/jats:p>\n          <\/jats:sec>","DOI":"10.1186\/1746-6148-5-2","type":"journal-article","created":{"date-parts":[[2009,1,8]],"date-time":"2009-01-08T19:21:17Z","timestamp":1231442477000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":98,"title":["Faecal shedding and strain diversity of Listeria monocytogenesin healthy ruminants and swine in Northern Spain"],"prefix":"10.1186","volume":"5","author":[{"given":"Jon I","family":"Esteban","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Beatriz","family":"Oporto","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gorka","family":"Aduriz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ram\u00f3n A","family":"Juste","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana","family":"Hurtado","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2009,1,8]]},"reference":[{"key":"136_CR1","volume-title":"Informe 2006. 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