{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T16:13:58Z","timestamp":1790439238998,"version":"4.1.0"},"reference-count":52,"publisher":"Springer Science and Business Media LLC","issue":"1","content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["BMC Vet Res"],"published-print":{"date-parts":[[2009,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n          <jats:sec>\n            <jats:title>Background<\/jats:title>\n            <jats:p>Control of brucellosis in livestock, wildlife and humans depends on the reliability of the methods used for detection and identification of bacteria. In the present study, we describe the evaluation of the recently established real-time PCR assay based on the <jats:italic>Brucella<\/jats:italic>-specific insertion sequence IS<jats:italic>711<\/jats:italic> with blood samples from 199 wild boars (first group of animals) and tissue samples from 53 wild boars (second group of animals) collected in Switzerland. Results from IS<jats:italic>711<\/jats:italic> real-time PCR were compared to those obtained by bacterial isolation, Rose Bengal Test (RBT), competitive ELISA (c-ELISA) and indirect ELISA (i-ELISA).<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Results<\/jats:title>\n            <jats:p>In the first group of animals, IS<jats:italic>711<\/jats:italic> real-time PCR detected infection in 11.1% (16\/144) of wild boars that were serologically negative. Serological tests showed different sensitivities [RBT 15.6%, c-ELISA 7.5% and i-ELISA 5.5%] and only 2% of blood samples were positive with all three tests, which makes interpretation of the serological results very difficult. Regarding the second group of animals, the IS<jats:italic>711<\/jats:italic> real-time PCR detected infection in 26% of animals, while <jats:italic>Brucella<\/jats:italic> spp. could be isolated from tissues of only 9.4% of the animals.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Conclusion<\/jats:title>\n            <jats:p>The results presented here indicate that IS<jats:italic>711<\/jats:italic> real-time PCR assay is a specific and sensitive tool for detection of <jats:italic>Brucella<\/jats:italic> spp. infections in wild boars. For this reason, we propose the employment of IS<jats:italic>711<\/jats:italic> real-time PCR as a complementary tool in brucellosis screening programs and for confirmation of diagnosis in doubtful cases.<\/jats:p>\n          <\/jats:sec>","DOI":"10.1186\/1746-6148-5-22","type":"journal-article","created":{"date-parts":[[2009,7,14]],"date-time":"2009-07-14T18:15:16Z","timestamp":1247595316000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":59,"title":["IS711-based real-time PCR assay as a tool for detection of Brucellaspp. in wild boars and comparison with bacterial isolation and serology"],"prefix":"10.1186","volume":"5","author":[{"given":"Vladimira","family":"Hini\u0107","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Isabelle","family":"Brodard","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andreas","family":"Thomann","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Milena","family":"Holub","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Raymond","family":"Miserez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carlos","family":"Abril","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2009,7,14]]},"reference":[{"key":"156_CR1","doi-asserted-by":"crossref","first-page":"115","DOI":"10.17221\/5684-VETMED","volume":"49","author":"Z Cvetnic","year":"2004","unstructured":"Cvetnic Z, Toncic J, Spicic S, Lojkic M, Terzic S, Jemersic L, Humski A, Curic S, Mitak M, Habrun B, et al: Brucellosis in wild boar (Sus scrofa) in the Republic of Croatia. Veterinarni Medicina. 2004, 49: 115-122.","journal-title":"Veterinarni Medicina"},{"key":"156_CR2","doi-asserted-by":"publisher","first-page":"362","DOI":"10.1136\/vr.160.11.362","volume":"160","author":"R Leuenberger","year":"2007","unstructured":"Leuenberger R, Boujon P, Th\u00fcr B, Miserez R, Garin-Bastuji B, R\u00fcfenacht J, St\u00e4rk KD: Prevalence of classical swine fever, Aujeszky's disease and brucellosis in a population of wild boar in Switzerland. Vet Rec. 2007, 160: 362-368.","journal-title":"Vet Rec"},{"key":"156_CR3","doi-asserted-by":"publisher","first-page":"212","DOI":"10.1007\/s10344-006-0080-0","volume":"53","author":"C K\u00f6ppel","year":"2007","unstructured":"K\u00f6ppel C, Knopf L, Ryser M-P, Miserez R, Th\u00fcr B, St\u00e4rk KDC: Serosurveillance for selected infectious disease agents in wild boars (Sus scrofa) and outdoor pigs in Switzerland. Eur J Wildl Res. 2007, 53: 212-220. 10.1007\/s10344-006-0080-0.","journal-title":"Eur J Wildl Res"},{"key":"156_CR4","doi-asserted-by":"publisher","first-page":"435","DOI":"10.1016\/S0378-1135(02)00228-6","volume":"90","author":"BJ Bricker","year":"2002","unstructured":"Bricker BJ: PCR as a diagnostic tool for brucellosis. Vet Microbiol. 2002, 90: 435-446. 10.1016\/S0378-1135(02)00228-6.","journal-title":"Vet Microbiol"},{"key":"156_CR5","doi-asserted-by":"publisher","first-page":"115","DOI":"10.1586\/14737159.4.1.115","volume":"4","author":"E Navarro","year":"2004","unstructured":"Navarro E, Casao MA, Solera J: Diagnosis of human brucellosis using PCR. Expert Rev Mol Diagn. 2004, 4: 115-123. 10.1586\/14737159.4.1.115.","journal-title":"Expert Rev Mol Diagn"},{"key":"156_CR6","doi-asserted-by":"publisher","first-page":"444","DOI":"10.1111\/j.1439-0450.2005.00898.x","volume":"52","author":"S Al Dahouk","year":"2005","unstructured":"Al Dahouk S, N\u00f6ckler K, Tomaso H, Splettstoesser WD, Jungersen G, Riber U, Petry T, Hoffmann D, Scholz HC, Hensel A, et al: Seroprevalence of brucellosis, tularemia, and yersiniosis in wild boars (Sus scrofa) from north-eastern Germany. J Vet Med B Infect Dis Vet Public Health. 2005, 52: 444-455.","journal-title":"J Vet Med B Infect Dis Vet Public Health"},{"key":"156_CR7","doi-asserted-by":"publisher","first-page":"1139","DOI":"10.1292\/jvms.68.1139","volume":"68","author":"M Watarai","year":"2006","unstructured":"Watarai M, Ito N, Omata Y, Ishiguro N: A serological survey of Brucella spp. in free-ranging wild boar (Sus scrofa leucomystax) in Shikoku, Japan. J Vet Med Sci. 2006, 68: 1139-1141. 10.1292\/jvms.68.1139.","journal-title":"J Vet Med Sci"},{"key":"156_CR8","doi-asserted-by":"publisher","first-page":"24","DOI":"10.1111\/j.1439-0450.2006.00899.x","volume":"53","author":"G Vengust","year":"2006","unstructured":"Vengust G, Valencak Z, Bidovec A: A serological survey of selected pathogens in wild boar in Slovenia. J Vet Med B Infect Dis Vet Public Health. 2006, 53: 24-27.","journal-title":"J Vet Med B Infect Dis Vet Public Health"},{"key":"156_CR9","doi-asserted-by":"publisher","first-page":"731","DOI":"10.1016\/j.theriogenology.2005.07.001","volume":"65","author":"F Ruiz-Fons","year":"2006","unstructured":"Ruiz-Fons F, Vicente J, Vidal D, H\u00f6fle U, Villan\u00faa D, Gauss C, Segal\u00e9s J, Almer\u00eda S, Montoro V, Gort\u00e1zar C: Seroprevalence of six reproductive pathogens in European wild boar (Sus scrofa) from Spain: the effect on wild boar female reproductive performance. Theriogenology. 2006, 65: 731-743. 10.1016\/j.theriogenology.2005.07.001.","journal-title":"Theriogenology"},{"key":"156_CR10","doi-asserted-by":"publisher","first-page":"271","DOI":"10.1016\/0378-1135(95)00121-2","volume":"47","author":"R Kittelberger","year":"1995","unstructured":"Kittelberger R, Hilbink F, Hansen MF, Ross GP, Joyce MA, Fenwick S, Heesemann J, Wolf-Watz H, Nielsen K: Serological crossreactivity between Brucella abortus and Yersinia enterocolitica 0:9 \u2013 II the use of Yersinia outer proteins for the specific detection of Yersinia enterocolitica infections in ruminants. Vet Microbiol. 1995, 47: 271-280. 10.1016\/0378-1135(95)00121-2.","journal-title":"Vet Microbiol"},{"key":"156_CR11","doi-asserted-by":"publisher","first-page":"461","DOI":"10.1016\/S0378-1135(02)00230-4","volume":"90","author":"J Godfroid","year":"2002","unstructured":"Godfroid J, Saegerman C, Wellemans V, Walravens K, Letesson J-J, Tibor A, Mc MA, Spencer S, Sanna M, Bakker D, et al: How to substantiate eradication of bovine brucellosis when aspecific serological reactions occur in the course of brucellosis testing. Vet Microbiol. 2002, 90: 461-477. 10.1016\/S0378-1135(02)00230-4.","journal-title":"Vet Microbiol"},{"key":"156_CR12","doi-asserted-by":"publisher","first-page":"34","DOI":"10.1016\/j.jim.2005.11.003","volume":"309","author":"S Al Dahouk","year":"2006","unstructured":"Al Dahouk S, N\u00f6ckler K, Scholz HC, Tomaso H, Bogumil R, Neubauer H: Immunoproteomic characterization of Brucella abortus 1119-3 preparations used for the serodiagnosis of Brucella infections. J Immunol Methods. 2006, 309: 34-47. 10.1016\/j.jim.2005.11.003.","journal-title":"J Immunol Methods"},{"key":"156_CR13","first-page":"141","volume":"12","author":"PM Mu\u00f1oz","year":"2005","unstructured":"Mu\u00f1oz PM, Mar\u00edn CM, Monreal D, Gonz\u00e1lez D, Garin-Bastuji B, D\u00edaz R, Mainar-Jaime RC, Moriy\u00f3n I, Blasco JM: Efficacy of several serological tests and antigens for diagnosis of bovine brucellosis in the presence of false-positive serological results due to Yersinia enterocolitica O:9. Clin Diagn Lab Immunol. 2005, 12: 141-151.","journal-title":"Clin Diagn Lab Immunol"},{"key":"156_CR14","doi-asserted-by":"crossref","first-page":"1939","DOI":"10.1128\/iai.65.5.1939-1943.1997","volume":"65","author":"V Weynants","year":"1997","unstructured":"Weynants V, Gilson D, Cloeckaert A, Tibor A, Denoel PA, Godfroid F, Limet JN, Letesson JJ: Characterization of smooth lipopolysaccharides and O polysaccharides of Brucella species by competition binding assays with monoclonal antibodies. Infect Immun. 1997, 65: 1939-1943.","journal-title":"Infect Immun"},{"key":"156_CR15","doi-asserted-by":"publisher","first-page":"1661","DOI":"10.1128\/JCM.39.4.1661-1664.2001","volume":"39","author":"L Zerva","year":"2001","unstructured":"Zerva L, Bourantas K, Mitka S, Kansouzidou A, Legakis NJ: Serum is the preferred clinical specimen for diagnosis of human brucellosis by PCR. J Clin Microbiol. 2001, 39: 1661-1664. 10.1128\/JCM.39.4.1661-1664.2001.","journal-title":"J Clin Microbiol"},{"key":"156_CR16","first-page":"577","volume":"49","author":"S Al Dahouk","year":"2003","unstructured":"Al Dahouk S, Tomaso H, N\u00f6ckler K, Neubauer H, Frangoulidis D: Laboratory-based diagnosis of brucellosis \u2013 a review of the literature. Part II: serological tests for brucellosis. Clin Lab. 2003, 49: 577-589.","journal-title":"Clin Lab"},{"key":"156_CR17","doi-asserted-by":"publisher","first-page":"60","DOI":"10.7589\/0090-3558-40.1.60","volume":"40","author":"SV Tessaro","year":"2004","unstructured":"Tessaro SV, Forbes LB: Experimental Brucella abortus infection in wolves. J Wildl Dis. 2004, 40: 60-65.","journal-title":"J Wildl Dis"},{"key":"156_CR18","volume-title":"Immunology and serology","author":"PL Carpenter","year":"1975","unstructured":"Carpenter PL: Immunology and serology. Philadelphia, PA: W. B. Saunders Company; 1975."},{"key":"156_CR19","doi-asserted-by":"publisher","first-page":"91","DOI":"10.1016\/S0378-1135(00)00200-5","volume":"75","author":"DS Leal-Klevezas","year":"2000","unstructured":"Leal-Klevezas DS, Mart\u00ednez-V\u00e1zquez IO, Garc\u00eda-Cant\u00fa J, L\u00f3pez-Merino A, Mart\u00ednez-Soriano JP: Use of polymerase chain reaction to detect Brucella abortus biovar 1 in infected goats. Vet Microbiol. 2000, 75: 91-97. 10.1016\/S0378-1135(00)00200-5.","journal-title":"Vet Microbiol"},{"key":"156_CR20","first-page":"5","volume":"40","author":"M Tittarelli","year":"2007","unstructured":"Tittarelli M, di Ventura M, de Massis F, Petrini A, Giovannini A, Nannini D, Caporale V: Kinetics of the antibody response in ewes experimentally infected with Brucella melitensis biovar 3. Veterinaria Italiana. 2007, 40: 5-10.","journal-title":"Veterinaria Italiana"},{"key":"156_CR21","doi-asserted-by":"publisher","first-page":"107","DOI":"10.1016\/S0167-5877(00)00119-7","volume":"45","author":"IA Gardner","year":"2000","unstructured":"Gardner IA, Stryhn H, Lind P, Collins MT: Conditional dependence between tests affects the diagnosis and surveillance of animal diseases. Prev Vet Med. 2000, 45: 107-122. 10.1016\/S0167-5877(00)00119-7.","journal-title":"Prev Vet Med"},{"key":"156_CR22","doi-asserted-by":"crossref","first-page":"277","DOI":"10.20506\/rst.21.2.1333","volume":"21","author":"J Godfroid","year":"2002","unstructured":"Godfroid J: Brucellosis in wildlife. Rev Sci Tech. 2002, 21: 277-286.","journal-title":"Rev Sci Tech"},{"key":"156_CR23","doi-asserted-by":"crossref","first-page":"2099","DOI":"10.1128\/aem.58.6.2099-2101.1992","volume":"58","author":"L Herman","year":"1992","unstructured":"Herman L, De RH: Identification of Brucella spp. by using the polymerase chain reaction. Appl Environ Microbiol. 1992, 58: 2099-2101.","journal-title":"Appl Environ Microbiol"},{"key":"156_CR24","doi-asserted-by":"crossref","first-page":"1683","DOI":"10.1128\/aem.62.5.1683-1688.1996","volume":"62","author":"NP Rijpens","year":"1996","unstructured":"Rijpens NP, Jannes G, Van Asbroeck M, Rossau R, Herman LM: Direct detection of Brucella spp. in raw milk by PCR and reverse hybridization with 16S-23S rRNA spacer probes. Appl Environ Microbiol. 1996, 62: 1683-1688.","journal-title":"Appl Environ Microbiol"},{"key":"156_CR25","first-page":"263","volume":"26","author":"DS Leal-Klevezas","year":"1995","unstructured":"Leal-Klevezas DS, Lop\u00e9z-Merino A, Mart\u00ednez-Soriano JP: Molecular detection of Brucella spp.: rapid identification of B. abortus biovar I using PCR. Arch Med Res. 1995, 26: 263-267.","journal-title":"Arch Med Res"},{"key":"156_CR26","first-page":"271","volume":"95","author":"GG Baily","year":"1992","unstructured":"Baily GG, Krahn JB, Drasar BS, Stoker NG: Detection of Brucella melitensis and Brucella abortus by DNA amplification. J Trop Med Hyg. 1992, 95: 271-275.","journal-title":"J Trop Med Hyg"},{"key":"156_CR27","doi-asserted-by":"crossref","first-page":"3087","DOI":"10.1128\/jcm.33.12.3087-3090.1995","volume":"33","author":"DS Leal-Klevezas","year":"1995","unstructured":"Leal-Klevezas DS, Mart\u00ednez-V\u00e1zquez IO, L\u00f3pez-Merino A, Mart\u00ednez-Soriano JP: Single-step PCR for detection of Brucella spp. from blood and milk of infected animals. J Clin Microbiol. 1995, 33: 3087-3090.","journal-title":"J Clin Microbiol"},{"key":"156_CR28","doi-asserted-by":"crossref","first-page":"2927","DOI":"10.1128\/jcm.35.11.2927-2930.1997","volume":"35","author":"MI Queipo-Ortu\u00f1o","year":"1997","unstructured":"Queipo-Ortu\u00f1o MI, Morata P, Oc\u00f3n P, Manchado P, Colmenero JD: Rapid diagnosis of human brucellosis by peripheral-blood PCR assay. J Clin Microbiol. 1997, 35: 2927-2930.","journal-title":"J Clin Microbiol"},{"key":"156_CR29","doi-asserted-by":"publisher","first-page":"239","DOI":"10.1136\/vr.144.9.239","volume":"144","author":"B Cetinkaya","year":"1999","unstructured":"Cetinkaya B, Ongor H, Muz A, Ertas HB, Kalender H, Erdogan HM: Detection of Brucella species DNA in the stomach content of aborted sheep fetuses by PCR. Vet Rec. 1999, 144: 239-240.","journal-title":"Vet Rec"},{"key":"156_CR30","doi-asserted-by":"publisher","first-page":"37","DOI":"10.1016\/S0378-1135(01)00401-1","volume":"83","author":"AS Amin","year":"2001","unstructured":"Amin AS, Hamdy ME, Ibrahim AK: Detection of Brucella melitensis in semen using the polymerase chain reaction assay. Vet Microbiol. 2001, 83: 37-44. 10.1016\/S0378-1135(01)00401-1.","journal-title":"Vet Microbiol"},{"key":"156_CR31","doi-asserted-by":"publisher","first-page":"147","DOI":"10.1111\/j.1574-695X.2002.tb00616.x","volume":"34","author":"E Navarro","year":"2002","unstructured":"Navarro E, Escribano J, Fernandez J, Solera J: Comparison of three different PCR methods for detection of Brucella spp in human blood samples. FEMS Immunol Med Microbiol. 2002, 34: 147-151. 10.1111\/j.1574-695X.2002.tb00616.x.","journal-title":"FEMS Immunol Med Microbiol"},{"key":"156_CR32","doi-asserted-by":"publisher","first-page":"299","DOI":"10.1053\/tvjl.2001.0681","volume":"163","author":"ME Hamdy","year":"2002","unstructured":"Hamdy ME, Amin AS: Detection of Brucella species in the milk of infected cattle, sheep, goats and camels by PCR. Vet J. 2002, 163: 299-305. 10.1053\/tvjl.2001.0681.","journal-title":"Vet J"},{"key":"156_CR33","doi-asserted-by":"publisher","first-page":"53","DOI":"10.1016\/S0378-1135(02)00442-X","volume":"93","author":"G Leyla","year":"2003","unstructured":"Leyla G, Kadri G, Umran O: Comparison of polymerase chain reaction and bacteriological culture for the diagnosis of sheep brucellosis using aborted fetus samples. Vet Microbiol. 2003, 93: 53-61. 10.1016\/S0378-1135(02)00442-X.","journal-title":"Vet Microbiol"},{"key":"156_CR34","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1016\/j.diagmicrobio.2005.03.011","volume":"53","author":"MG Elfaki","year":"2005","unstructured":"Elfaki MG, Uz-Zaman T, Al-Hokail AA, Nakeeb SM: Detection of Brucella DNA in sera from patients with brucellosis by polymerase chain reaction. Diagn Microbiol Infect Dis. 2005, 53: 1-7. 10.1016\/j.diagmicrobio.2005.03.011.","journal-title":"Diagn Microbiol Infect Dis"},{"key":"156_CR35","doi-asserted-by":"publisher","first-page":"1203","DOI":"10.1016\/j.theriogenology.2007.01.003","volume":"67","author":"LB Keid","year":"2007","unstructured":"Keid LB, Soares RM, Vasconcellos SA, Chiebao DP, Megid J, Salgado VR, Richtzenhain LJ: A polymerase chain reaction for the detection of Brucella canis in semen of naturally infected dogs. Theriogenology. 2007, 67: 1203-1210. 10.1016\/j.theriogenology.2007.01.003.","journal-title":"Theriogenology"},{"key":"156_CR36","doi-asserted-by":"crossref","first-page":"3198","DOI":"10.1128\/jcm.33.12.3198-3200.1995","volume":"33","author":"C Romero","year":"1995","unstructured":"Romero C, Pardo M, Grillo MJ, Diaz R, Blasco JM, Lopez-Go\u00f1i I: Evaluation of PCR and indirect enzyme-linked immunosorbent assay on milk samples for diagnosis of brucellosis in dairy cattle. J Clin Microbiol. 1995, 33: 3198-3200.","journal-title":"J Clin Microbiol"},{"key":"156_CR37","doi-asserted-by":"publisher","first-page":"4753","DOI":"10.1128\/AEM.69.8.4753-4759.2003","volume":"69","author":"DT Newby","year":"2003","unstructured":"Newby DT, Hadfield TL, Roberto FF: Real-time PCR detection of Brucella abortus: a comparative study of SYBR green I, 5'-exonuclease, and hybridization probe assays. Appl Environ Microbiol. 2003, 69: 4753-4759. 10.1128\/AEM.69.8.4753-4759.2003.","journal-title":"Appl Environ Microbiol"},{"key":"156_CR38","doi-asserted-by":"publisher","first-page":"1290","DOI":"10.1128\/JCM.42.3.1290-1293.2004","volume":"42","author":"WS Probert","year":"2004","unstructured":"Probert WS, Schrader KN, Khuong NY, Bystrom SL, Graves MH: Real-time multiplex PCR assay for detection of Brucella spp., B. abortus, and B. melitensis. J Clin Microbiol. 2004, 42: 1290-1293. 10.1128\/JCM.42.3.1290-1293.2004.","journal-title":"J Clin Microbiol"},{"key":"156_CR39","doi-asserted-by":"publisher","first-page":"2261","DOI":"10.1128\/JCM.42.5.2261-2263.2004","volume":"42","author":"T Bogdanovich","year":"2004","unstructured":"Bogdanovich T, Skurnik M, L\u00fcbeck PS, Ahrens P, Hoorfar J: Validated 5' nuclease PCR assay for rapid identification of the genus Brucella. J Clin Microbiol. 2004, 42: 2261-2263. 10.1128\/JCM.42.5.2261-2263.2004.","journal-title":"J Clin Microbiol"},{"key":"156_CR40","doi-asserted-by":"publisher","first-page":"1025","DOI":"10.1136\/jnnp.2004.049411","volume":"76","author":"JD Colmenero","year":"2005","unstructured":"Colmenero JD, Queipo-Ortu\u00f1o MI, Reguera JM, Baeza G, Salazar JA, Morata P: Real time polymerase chain reaction: a new powerful tool for the diagnosis of neurobrucellosis. J Neurol Neurosurg Psychiatry. 2005, 76: 1025-1027. 10.1136\/jnnp.2004.049411.","journal-title":"J Neurol Neurosurg Psychiatry"},{"key":"156_CR41","doi-asserted-by":"publisher","first-page":"842","DOI":"10.1007\/s10096-005-0064-0","volume":"24","author":"C Debeaumont","year":"2005","unstructured":"Debeaumont C, Falconnet PA, Maurin M: Real-time PCR for detection of Brucella spp. DNA in human serum samples. Eur J Clin Microbiol Infect Dis. 2005, 24: 842-845. 10.1007\/s10096-005-0064-0.","journal-title":"Eur J Clin Microbiol Infect Dis"},{"key":"156_CR42","doi-asserted-by":"publisher","first-page":"260","DOI":"10.1086\/426818","volume":"40","author":"MI Queipo-Ortu\u00f1o","year":"2005","unstructured":"Queipo-Ortu\u00f1o MI, Colmenero JD, Baeza G, Morata P: Comparison between LightCycler Real-Time Polymerase Chain Reaction (PCR) assay with serum and PCR-enzyme-linked immunosorbent assay with whole blood samples for the diagnosis of human brucellosis. Clin Infect Dis. 2005, 40: 260-264. 10.1086\/426818.","journal-title":"Clin Infect Dis"},{"key":"156_CR43","doi-asserted-by":"publisher","first-page":"713","DOI":"10.1111\/j.1469-0691.2005.01202.x","volume":"11","author":"MI Queipo-Ortu\u00f1o","year":"2005","unstructured":"Queipo-Ortu\u00f1o MI, Colmenero JD, Reguera JM, Garc\u00eda-Ordo\u00f1ez MA, Pach\u00f3n ME, Gonzalez M, Morata P: Rapid diagnosis of human brucellosis by SYBR Green I-based real-time PCR assay and melting curve analysis in serum samples. Clin Microbiol Infect. 2005, 11: 713-718. 10.1111\/j.1469-0691.2005.01202.x.","journal-title":"Clin Microbiol Infect"},{"key":"156_CR44","doi-asserted-by":"publisher","first-page":"2290","DOI":"10.1016\/j.juro.2006.07.052","volume":"176","author":"MI Queipo-Ortu\u00f1o","year":"2006","unstructured":"Queipo-Ortu\u00f1o MI, Colmenero JD, Mu\u00f1oz N, Baeza G, Clavijo E, Morata P: Rapid diagnosis of Brucella epididymo-orchitis by real-time polymerase chain reaction assay in urine samples. J Urol. 2006, 176: 2290-2293. 10.1016\/j.juro.2006.07.052.","journal-title":"J Urol"},{"key":"156_CR45","doi-asserted-by":"publisher","first-page":"170","DOI":"10.1016\/j.rvsc.2005.12.001","volume":"81","author":"S O'Leary","year":"2006","unstructured":"O'Leary S, Sheahan M, Sweeney T: Brucella abortus detection by PCR assay in blood, milk and lymph tissue of serologically positive cows. Res Vet Sci. 2006, 81: 170-176. 10.1016\/j.rvsc.2005.12.001.","journal-title":"Res Vet Sci"},{"key":"156_CR46","doi-asserted-by":"publisher","first-page":"375","DOI":"10.1016\/j.mimet.2008.07.002","volume":"75","author":"V Hinic","year":"2008","unstructured":"Hinic V, Brodard I, Thomann A, Cvetnic Z, Makaya PV, Frey J, Abril C: Novel identification and differentiation of Brucella melitensis, B. abortus, B. suis, B. ovis, B. canis, and B. neotomae suitable for both conventional and real-time PCR systems. J Microbiol Meth. 2008, 75: 375-378. 10.1016\/j.mimet.2008.07.002.","journal-title":"J Microbiol Meth"},{"key":"156_CR47","doi-asserted-by":"crossref","first-page":"760","DOI":"10.1128\/CDLI.6.5.760-764.1999","volume":"6","author":"R Vemulapalli","year":"1999","unstructured":"Vemulapalli R, McQuiston JR, Schurig GG, Sriranganathan N, Halling SM, Boyle SM: Identification of an IS711 element interrupting the wboA gene of Brucella abortus vaccine strain RB51 and a PCR assay to distinguish strain RB51 from other Brucella species and strains. Clin Diagn Lab Immunol. 1999, 6: 760-764.","journal-title":"Clin Diagn Lab Immunol"},{"key":"156_CR48","doi-asserted-by":"publisher","first-page":"209","DOI":"10.1016\/S0378-1135(02)00210-9","volume":"90","author":"E Moreno","year":"2002","unstructured":"Moreno E, Cloeckaert A, Moriy\u00f3n I: Brucella evolution and taxonomy. Vet Microbiol. 2002, 90: 209-227. 10.1016\/S0378-1135(02)00210-9.","journal-title":"Vet Microbiol"},{"key":"156_CR49","doi-asserted-by":"crossref","first-page":"2443","DOI":"10.1128\/JCM.36.9.2443-2446.1998","volume":"36","author":"P Morata","year":"1998","unstructured":"Morata P, Queipo-Ortu\u00f1o MI, de Dios Colmenero J: Strategy for optimizing DNA amplification in a peripheral blood PCR assay used for diagnosis of human brucellosis. J Clin Microbiol. 1998, 36: 2443-2446.","journal-title":"J Clin Microbiol"},{"key":"156_CR50","first-page":"MT69","volume":"11","author":"MG Elfaki","year":"2005","unstructured":"Elfaki MG, Al-Hokail AA, Nakeeb SM, Al-Rabiah FA: Evaluation of culture, tube agglutination, and PCR methods for the diagnosis of brucellosis in humans. Med Sci Monit. 2005, 11: MT69-MT74.","journal-title":"Med Sci Monit"},{"key":"156_CR51","doi-asserted-by":"publisher","first-page":"301","DOI":"10.1111\/j.1472-765X.2007.02309.x","volume":"46","author":"Z Ilhan","year":"2008","unstructured":"Ilhan Z, Aksakal A, Ekin IH, G\u00fclhan T, Solmaz H, Erdenlig S: Comparison of culture and PCR for the detection of Brucella melitensis in blood and lymphoid tissues of serologically positive and negative slaughtered sheep. Lett Appl Microbiol. 2008, 46: 301-306. 10.1111\/j.1472-765X.2007.02309.x.","journal-title":"Lett Appl Microbiol"},{"key":"156_CR52","volume-title":"Manual of standards for diagnostic tests and vaccines","author":"Office International des Epizooties OIE","year":"2008","unstructured":"Office International des Epizooties OIE: Manual of standards for diagnostic tests and vaccines. Paris: Office International des Epizooties O.I.E; 2008."}],"container-title":["BMC Veterinary Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1186\/1746-6148-5-22.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,8,31]],"date-time":"2021-08-31T22:54:17Z","timestamp":1630450457000},"score":1,"resource":{"primary":{"URL":"https:\/\/bmcvetres.biomedcentral.com\/articles\/10.1186\/1746-6148-5-22"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2009,7,14]]},"references-count":52,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2009,12]]}},"alternative-id":["156"],"URL":"https:\/\/doi.org\/10.1186\/1746-6148-5-22","relation":{},"ISSN":["1746-6148"],"issn-type":[{"value":"1746-6148","type":"electronic"}],"subject":[],"published":{"date-parts":[[2009,7,14]]},"assertion":[{"value":"10 December 2008","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"14 July 2009","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"14 July 2009","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}}],"article-number":"22"}}