{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,14]],"date-time":"2026-11-14T08:14:16Z","timestamp":1794644056385,"version":"4.9.1"},"reference-count":20,"publisher":"Springer Science and Business Media LLC","issue":"1","content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["BMC Vet Res"],"published-print":{"date-parts":[[2012,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>\n                      Contagious Agalactia (CA) is one of the major animal health problems in small ruminants because of its economic significance. Currently, four\n                      <jats:italic>Mycoplasma<\/jats:italic>\n                      spp. have been associated with this syndrome:\n                      <jats:italic>M. agalactiae<\/jats:italic>\n                      ,\n                      <jats:italic>M. mycoides<\/jats:italic>\n                      subsp.\n                      <jats:italic>capri<\/jats:italic>\n                      ,\n                      <jats:italic>M. capricolum<\/jats:italic>\n                      subsp.\n                      <jats:italic>capricolum<\/jats:italic>\n                      and\n                      <jats:italic>M. putrefaciens<\/jats:italic>\n                      . Their presence has been evaluated in several studies conducted in CA-endemic countries. However, previous Spanish studies have been focused on caprine CA, and there is a knowledge gap regarding which\n                      <jats:italic>Mycoplasma<\/jats:italic>\n                      species are present in sheep flocks from Spain, which has the second highest number of sheep amongst the 27 European Union member states. Consequently, we investigated the presence and geographic distribution of the four CA-causing mycoplasmas in Spanish dairy sheep farms. This is the first time such an investigation has been performed.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Three hundred thirty nine out of 922 sheep flocks were positive for\n                      <jats:italic>M. agalactiae<\/jats:italic>\n                      by real time PCR (36.8%) and 85 by microbiological identification (9.2%). Interestingly, all 597 milk samples assessed for the presence of\n                      <jats:italic>M. mycoides<\/jats:italic>\n                      subsp.\n                      <jats:italic>capri<\/jats:italic>\n                      ,\n                      <jats:italic>M. capricolum<\/jats:italic>\n                      subsp.\n                      <jats:italic>capricolum<\/jats:italic>\n                      and\n                      <jats:italic>M<\/jats:italic>\n                      .\n                      <jats:italic>putrefaciens<\/jats:italic>\n                      tested negative. To evaluate the intermittent excretion of the pathogen in milk, we sampled 391 additional farms from 2 to 5 times, resulting that in 26.3% of the cases a previously positive farm tested negative in a later sampling.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>\n                      <jats:italic>M<\/jats:italic>\n                      .\n                      <jats:italic>agalactiae<\/jats:italic>\n                      was the only\n                      <jats:italic>Mycoplasma<\/jats:italic>\n                      species detected in the study area showing a high frequency of presence and wide distribution. Therefore, the establishment of a permanent surveillance network is advantageous, as well as the implementation of control and prevention measures to hinder the dissemination of\n                      <jats:italic>M. agalactiae<\/jats:italic>\n                      and to prevent the entrance of other\n                      <jats:italic>Mycoplasma<\/jats:italic>\n                      species.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1186\/1746-6148-8-171","type":"journal-article","created":{"date-parts":[[2012,9,24]],"date-time":"2012-09-24T08:14:25Z","timestamp":1348474465000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":24,"title":["A survey of Mycoplasma agalactiaein dairy sheep farms in Spain"],"prefix":"10.1186","volume":"8","author":[{"given":"Jaime","family":"Ariza-Miguel","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David","family":"Rodr\u00edguez-L\u00e1zaro","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marta","family":"Hern\u00e1ndez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2012,9,24]]},"reference":[{"key":"478_CR1","doi-asserted-by":"publisher","first-page":"32","DOI":"10.1186\/1746-6148-6-32","volume":"6","author":"M Chazel","year":"2000","unstructured":"Chazel M, Tardy F, Le Grand D, Calavas D, Poumarat F: Mycoplasmoses of ruminants in France: recent data from the national surveillance network. 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