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The relationship between change in nociceptive thermal threshold and the opioid\u2032s plasma concentration was determined. Six adult beagle dogs received levomethadone (0.2 mg\/kg), acepromazine (0.02 mg\/kg) or saline placebo by intramuscular injection (IM) in a randomized cross-over design. Three baseline nociceptive thermal threshold readings were taken at 15 minutes intervals prior to treatment. Further readings were made at 15, 30, 45, 60, 90, 120, 150, 180, 210, 240, 270, 300, 330, 360, 420 and 480 minutes after injection. A sedation score was assigned at every reading. Four saline placebo treatments were performed to assess baseline reproducibility. Levomethadone serum concentrations were measured prior and 0.5, 1, 2, 4, 8, 12 and 24 hours after drug dosing in a separate occasion.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Acepromazine did not seem to increase the thermal threshold at any time. After levomethadone there was a significant rise of the thermal threshold between 15 to 120 minutes at serum concentrations between 22.6-46.3 ng\/mL. Baseline reproducibility was stable in adult beagle dogs.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>The thermal threshold testing system is a suitable device for nociceptive threshold testing in dogs.<\/jats:p><\/jats:sec>","DOI":"10.1186\/1746-6148-8-206","type":"journal-article","created":{"date-parts":[[2012,10,31]],"date-time":"2012-10-31T17:15:01Z","timestamp":1351703701000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["Contact heat thermal threshold testing in beagle dogs: baseline reproducibility and the effect of acepromazine, levomethadone and fenpipramide"],"prefix":"10.1186","volume":"8","author":[{"given":"Marina Verena","family":"Hoffmann","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sabine Beate Rita","family":"K\u00e4stner","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Manfred","family":"Kietzmann","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sabine","family":"Kramer","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2012,10,30]]},"reference":[{"key":"540_CR1","doi-asserted-by":"publisher","first-page":"205","DOI":"10.1053\/rvsc.2001.0543","volume":"72","author":"MJ Dixon","year":"2002","unstructured":"Dixon MJ, Robertson SA, Taylor PM: A thermal threshold testing device for evaluation of analgesics in cats. 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