{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,3]],"date-time":"2026-11-03T22:25:07Z","timestamp":1793744707745,"version":"4.6.1"},"reference-count":37,"publisher":"Springer Science and Business Media LLC","issue":"1","content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["BMC Vet Res"],"published-print":{"date-parts":[[2013,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Osteoarthritis (OA) is a common joint disease that causes disabilities in elderly adults. However, few long-lasting pharmacotherapeutic agents with low side effects have been developed to treat OA. We evaluated the therapeutic effects of intra-articular injections of hydrogels containing hyaluronic acid (HA) and doxycycline (DOX) in a rabbit OA model.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Thirteen week old New Zealand White rabbits undergone a partial meniscectomy and unilateral fibular ligament transection were administered with either normal saline (NT), HA, DOX or HA-DOX hydrogels on day 0, 3, 6, 9 and 12; animals were also examined the pain assessment in every three days. The joint samples were taken at day 14 post-surgery for further histopathological evaluation. The degree of pain was significantly attenuated after day 7 post-treatment with both HA and HA-DOX hydrogels. In macroscopic appearance, HA-DOX hydrogel group showed a smoother cartilage surface, no or minimal signs of ulceration, smaller osteophytes, and less fissure formation in compare to HA or DOX treatment alone. In the areas with slight OA changes, HA-DOX hydrogel group exhibited normal distribution of chondrocytes, indicating the existence of cartilage regeneration. In addition, HA-DOX hydrogels also ameliorated the progression of OA by protecting the injury of articular cartilage layer and restoring the elastoviscosity.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Overall, from both macroscopic and microscopic data of this study indicate the injectable HA-DOX hydrogels presented as a long-lasting pharmacotherapeutic agent to apply for OA therapy.<\/jats:p><\/jats:sec>","DOI":"10.1186\/1746-6148-9-68","type":"journal-article","created":{"date-parts":[[2013,4,10]],"date-time":"2013-04-10T12:15:17Z","timestamp":1365596117000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":42,"title":["Injectable hyaluronic-acid-doxycycline hydrogel therapy in experimental rabbit osteoarthritis"],"prefix":"10.1186","volume":"9","author":[{"given":"Hsien-Tsung","family":"Lu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ming-Thau","family":"Sheu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yung-Feng","family":"Lin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jai","family":"Lan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi-Ping","family":"Chin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ming-Shium","family":"Hsieh","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chao-Wen","family":"Cheng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chien-Ho","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2013,4,10]]},"reference":[{"issue":"10","key":"638_CR1","doi-asserted-by":"publisher","first-page":"3160","DOI":"10.1002\/art.21345","volume":"52","author":"LS Lohmander","year":"2005","unstructured":"Lohmander LS, Brandt KD, Mazzuca SA, Katz BP, Larsson S, Struglics A, Lane KA: Use of the plasma stromelysin (matrix metalloproteinase 3) concentration to predict joint space narrowing in knee osteoarthritis. Arthritis rheumatism. 2005, 52 (10): 3160-3167. 10.1002\/art.21345.","journal-title":"Arthritis rheumatism"},{"issue":"1","key":"638_CR2","doi-asserted-by":"publisher","first-page":"27","DOI":"10.1016\/j.joca.2006.06.012","volume":"15","author":"CM Thomas","year":"2007","unstructured":"Thomas CM, Fuller CJ, Whittles CE, Sharif M: Chondrocyte death by apoptosis is associated with cartilage matrix degradation. Osteoarthritis cartil OARS Osteoarthritis Res Soc. 2007, 15 (1): 27-34. 10.1016\/j.joca.2006.06.012.","journal-title":"Osteoarthritis cartil OARS Osteoarthritis Res Soc"},{"issue":"6","key":"638_CR3","doi-asserted-by":"crossref","first-page":"3160","DOI":"10.4049\/jimmunol.162.6.3160","volume":"162","author":"MG Attur","year":"1999","unstructured":"Attur MG, Patel RN, Patel PD, Abramson SB, Amin AR: Tetracycline up-regulates COX-2 expression and prostaglandin E2 production independent of its effect on nitric oxide. J Immunol. 1999, 162 (6): 3160-3167.","journal-title":"J Immunol"},{"issue":"2","key":"638_CR4","doi-asserted-by":"publisher","first-page":"129","DOI":"10.1093\/rheumatology\/kei171","volume":"45","author":"G Verbruggen","year":"2006","unstructured":"Verbruggen G: Chondroprotective drugs in degenerative joint diseases. Rheumatology. 2006, 45 (2): 129-138.","journal-title":"Rheumatology"},{"issue":"2","key":"638_CR5","doi-asserted-by":"publisher","first-page":"206","DOI":"10.1186\/ar1932","volume":"8","author":"JP Pelletier","year":"2006","unstructured":"Pelletier JP, Martel-Pelletier J, Raynauld JP: Most recent developments in strategies to reduce the progression of structural changes in osteoarthritis: today and tomorrow. Arthritis res therap. 2006, 8 (2): 206-10.1186\/ar1932.","journal-title":"Arthritis res therap"},{"issue":"Suppl A","key":"638_CR6","doi-asserted-by":"publisher","first-page":"S63","DOI":"10.1016\/j.joca.2003.09.014","volume":"12","author":"JP Pelletier","year":"2004","unstructured":"Pelletier JP: Rationale for the use of structure-modifying drugs and agents in the treatment of osteoarthritis. Osteoarthritis and cartilage\/OARS. Osteoarthritis Res Soc. 2004, 12 (Suppl A): S63-S68.","journal-title":"Osteoarthritis Res Soc"},{"issue":"3","key":"638_CR7","doi-asserted-by":"publisher","first-page":"256","DOI":"10.1097\/01.bor.0000218945.96988.0a","volume":"18","author":"MS Hogenmiller","year":"2006","unstructured":"Hogenmiller MS, Lozada CJ: An update on osteoarthritis therapeutics. Curr Opin Rheumatol. 2006, 18 (3): 256-260. 10.1097\/01.bor.0000218945.96988.0a.","journal-title":"Curr Opin Rheumatol"},{"issue":"1","key":"638_CR8","doi-asserted-by":"publisher","first-page":"217","DOI":"10.1016\/j.rdc.2005.11.002","volume":"32","author":"KD Brandt","year":"2006","unstructured":"Brandt KD, Mazzuca SA: Experience with a placebo-controlled randomized clinical trial of a disease-modifying drug for osteoarthritis: the doxycycline trial. Rheum Dis Clin N Am. 2006, 32 (1): 217-234. 10.1016\/j.rdc.2005.11.002. xi-xii.","journal-title":"Rheum Dis Clin N Am"},{"issue":"11","key":"638_CR9","doi-asserted-by":"publisher","first-page":"1189","DOI":"10.1016\/j.joca.2006.04.009","volume":"14","author":"MP Hellio Le Graverand","year":"2006","unstructured":"Hellio Le Graverand MP, Brandt KD, Mazzuca SA, Katz BP, Buck R, Lane KA, Pickering E, Nemirovskiy OV, Sunyer T, Welsch DJ: Association between concentrations of urinary type II collagen neoepitope (uTIINE) and joint space narrowing in patients with knee osteoarthritis. Osteoarthritis and cartilage\/OARS. Osteoarthritis Res Soc. 2006, 14 (11): 1189-1195. 10.1016\/j.joca.2006.04.009.","journal-title":"Osteoarthritis Res Soc"},{"issue":"7","key":"638_CR10","doi-asserted-by":"publisher","first-page":"2015","DOI":"10.1002\/art.21122","volume":"52","author":"KD Brandt","year":"2005","unstructured":"Brandt KD, Mazzuca SA, Katz BP, Lane KA, Buckwalter KA, Yocum DE, Wolfe F, Schnitzer TJ, Moreland LW, Manzi S: Effects of doxycycline on progression of osteoarthritis: results of a randomized, placebo-controlled, double-blind trial. Arthritis rheumatism. 2005, 52 (7): 2015-2025. 10.1002\/art.21122.","journal-title":"Arthritis rheumatism"},{"issue":"4","key":"638_CR11","doi-asserted-by":"publisher","first-page":"1254","DOI":"10.1152\/japplphysiol.00122.2004","volume":"97","author":"CK Pardy","year":"2004","unstructured":"Pardy CK, Matyas JR, Zernicke RF: Doxycycline effects on mechanical and morphometrical properties of early- and late-stage osteoarthritic bone following anterior cruciate ligament injury. J Appl Physiol. 2004, 97 (4): 1254-1260. 10.1152\/japplphysiol.00122.2004.","journal-title":"J Appl Physiol"},{"issue":"24","key":"638_CR12","doi-asserted-by":"publisher","first-page":"14014","DOI":"10.1073\/pnas.93.24.14014","volume":"93","author":"AR Amin","year":"1996","unstructured":"Amin AR, Attur MG, Thakker GD, Patel PD, Vyas PR, Patel RN, Patel IR, Abramson SB: A novel mechanism of action of tetracyclines: effects on nitric oxide synthases. Proc Natl Acad Sci U S A. 1996, 93 (24): 14014-14019. 10.1073\/pnas.93.24.14014.","journal-title":"Proc Natl Acad Sci U S A"},{"issue":"2\u20133","key":"638_CR13","doi-asserted-by":"publisher","first-page":"259","DOI":"10.1016\/S0014-5793(97)00605-4","volume":"410","author":"AR Amin","year":"1997","unstructured":"Amin AR, Patel RN, Thakker GD, Lowenstein CJ, Attur MG, Abramson SB: Post-transcriptional regulation of inducible nitric oxide synthase mRNA in murine macrophages by doxycycline and chemically modified tetracyclines. FEBS Lett. 1997, 410 (2\u20133): 259-264.","journal-title":"FEBS Lett"},{"issue":"8","key":"638_CR14","doi-asserted-by":"publisher","first-page":"409","DOI":"10.1007\/PL00000263","volume":"50","author":"D Borderie","year":"2001","unstructured":"Borderie D, Hernvann A, Hilliquin P, Lemarchal H, Kahan A, Ekindjian OG: Tetracyclines inhibit nitrosothiol production by cytokine-stimulated osteoarthritic synovial cells. Inflammation Res off J European Histamine Res Soc [et al]. 2001, 50 (8): 409-414. 10.1007\/PL00000263.","journal-title":"Inflammation Res off J European Histamine Res Soc [et al]"},{"issue":"6","key":"638_CR15","doi-asserted-by":"crossref","first-page":"3459","DOI":"10.4049\/jimmunol.163.6.3459","volume":"163","author":"RN Patel","year":"1999","unstructured":"Patel RN, Attur MG, Dave MN, Patel IV, Stuchin SA, Abramson SB, Amin AR: A novel mechanism of action of chemically modified tetracyclines: inhibition of COX-2-mediated prostaglandin E2 production. J Immunol. 1999, 163 (6): 3459-3467.","journal-title":"J Immunol"},{"issue":"3","key":"638_CR16","doi-asserted-by":"publisher","first-page":"239","DOI":"10.4142\/jvs.2009.10.3.239","volume":"10","author":"K Nganvongpanit","year":"2009","unstructured":"Nganvongpanit K, Pothacharoen P, Suwankong N, Ong-Chai S, Kongtawelert P: The effect of doxycycline on canine hip osteoarthritis: design of a 6-months clinical trial. J Vet Sci. 2009, 10 (3): 239-247. 10.4142\/jvs.2009.10.3.239.","journal-title":"J Vet Sci"},{"issue":"1","key":"638_CR17","doi-asserted-by":"publisher","first-page":"123","DOI":"10.1016\/j.biocel.2005.08.011","volume":"38","author":"S Iacob","year":"2006","unstructured":"Iacob S, Knudson CB: Hyaluronan fragments activate nitric oxide synthase and the production of nitric oxide by articular chondrocytes. Int J Biochem Cell Biol. 2006, 38 (1): 123-133. 10.1016\/j.biocel.2005.08.011.","journal-title":"Int J Biochem Cell Biol"},{"issue":"26","key":"638_CR18","doi-asserted-by":"publisher","first-page":"17952","DOI":"10.1074\/jbc.M602750200","volume":"281","author":"S Ohno","year":"2006","unstructured":"Ohno S, Im HJ, Knudson CB, Knudson W: Hyaluronan oligosaccharides induce matrix metalloproteinase 13 via transcriptional activation of NFkappaB and p38 MAP kinase in articular chondrocytes. J Biol Chem. 2006, 281 (26): 17952-17960. 10.1074\/jbc.M602750200.","journal-title":"J Biol Chem"},{"issue":"12","key":"638_CR19","doi-asserted-by":"publisher","first-page":"3307","DOI":"10.1185\/03007990802490124","volume":"24","author":"D Brzusek","year":"2008","unstructured":"Brzusek D, Petron D: Treating knee osteoarthritis with intra-articular hyaluronans. Current med res opinion. 2008, 24 (12): 3307-3322. 10.1185\/03007990802490124.","journal-title":"Current med res opinion"},{"issue":"2","key":"638_CR20","doi-asserted-by":"publisher","first-page":"182","DOI":"10.1053\/joca.1998.0207","volume":"7","author":"K Takahashi","year":"1999","unstructured":"Takahashi K, Goomer RS, Harwood F, Kubo T, Hirasawa Y, Amiel D: The effects of hyaluronan on matrix metalloproteinase-3 (MMP-3), interleukin-1beta(IL-1beta), and tissue inhibitor of metalloproteinase-1 (TIMP-1) gene expression during the development of osteoarthritis. Osteoarthr cartil OARS Osteoarthr Res Soc. 1999, 7 (2): 182-190. 10.1053\/joca.1998.0207.","journal-title":"Osteoarthr cartil OARS Osteoarthr Res Soc"},{"issue":"4","key":"638_CR21","doi-asserted-by":"publisher","first-page":"251","DOI":"10.1016\/S1063-4584(97)80021-0","volume":"5","author":"M Yoshioka","year":"1997","unstructured":"Yoshioka M, Shimizu C, Harwood FL, Coutts RD, Amiel D: The effects of hyaluronan during the development of osteoarthritis. Osteoarthr cartil OARS Osteoarthr Res Soc. 1997, 5 (4): 251-260. 10.1016\/S1063-4584(97)80021-0.","journal-title":"Osteoarthr cartil OARS Osteoarthr Res Soc"},{"issue":"1","key":"638_CR22","doi-asserted-by":"publisher","first-page":"149","DOI":"10.1002\/jbm.a.30078","volume":"70","author":"N Kawai","year":"2004","unstructured":"Kawai N, Tanaka E, Takata T, Miyauchi M, Tanaka M, Todoh M, van Eijden T, Tanne K: Influence of additive hyaluronic acid on the lubricating ability in the temporomandibular joint. J biomed mat res Part A. 2004, 70 (1): 149-153.","journal-title":"J biomed mat res Part A"},{"issue":"7","key":"638_CR23","doi-asserted-by":"publisher","first-page":"1923","DOI":"10.1002\/art.11172","volume":"48","author":"T Kawano","year":"2003","unstructured":"Kawano T, Miura H, Mawatari T, Moro-Oka T, Nakanishi Y, Higaki H, Iwamoto Y: Mechanical effects of the intraarticular administration of high molecular weight hyaluronic acid plus phospholipid on synovial joint lubrication and prevention of articular cartilage degeneration in experimental osteoarthritis. Arthritis rheumatism. 2003, 48 (7): 1923-1929. 10.1002\/art.11172.","journal-title":"Arthritis rheumatism"},{"issue":"9","key":"638_CR24","doi-asserted-by":"publisher","first-page":"1549","DOI":"10.1016\/S0149-2918(00)80010-7","volume":"21","author":"M Wobig","year":"1999","unstructured":"Wobig M, Bach G, Beks P, Dickhut A, Runzheimer J, Schwieger G, Vetter G, Balazs E: The role of elastoviscosity in the efficacy of viscosupplementation for osteoarthritis of the knee: a comparison of hylan G-F 20 and a lower-molecular-weight hyaluronan. Clin Ther. 1999, 21 (9): 1549-1562. 10.1016\/S0149-2918(00)80010-7.","journal-title":"Clin Ther"},{"key":"638_CR25","doi-asserted-by":"publisher","first-page":"457","DOI":"10.1002\/jbm.b.31971","volume":"100B","author":"NE Larsen","year":"2012","unstructured":"Larsen NE, Dursema HD, Pollak CT, Skrabut EM: Clearance kinetics of a hylan-based viscosupplement after intra-articular and intravenous administration in animal models. J biomed mat res Part B Applied biomat. 2012, 100B: 457-462. 10.1002\/jbm.b.31971.","journal-title":"J biomed mat res Part B Applied biomat"},{"issue":"1","key":"638_CR26","doi-asserted-by":"publisher","first-page":"125","DOI":"10.1113\/expphysiol.1991.sp003474","volume":"76","author":"TJ Brown","year":"1991","unstructured":"Brown TJ, Laurent UB, Fraser JR: Turnover of hyaluronan in synovial joints: elimination of labelled hyaluronan from the knee joint of the rabbit. Exp Physiol. 1991, 76 (1): 125-134.","journal-title":"Exp Physiol"},{"issue":"3","key":"638_CR27","doi-asserted-by":"publisher","first-page":"337","DOI":"10.1016\/S0168-3659(01)00453-9","volume":"76","author":"T Miyazaki","year":"2001","unstructured":"Miyazaki T, Yomota C, Okada S: Development and release characterization of hyaluronan-doxycycline gels based on metal coordination. J Contr Release Off J Contr Release Soc. 2001, 76 (3): 337-347. 10.1016\/S0168-3659(01)00453-9.","journal-title":"J Contr Release Off J Contr Release Soc"},{"issue":"1\u20132","key":"638_CR28","doi-asserted-by":"publisher","first-page":"126","DOI":"10.1016\/j.pain.2006.11.012","volume":"130","author":"A Gomis","year":"2007","unstructured":"Gomis A, Miralles A, Schmidt RF, Belmonte C: Nociceptive nerve activity in an experimental model of knee joint osteoarthritis of the guinea pig: effect of intra-articular hyaluronan application. Pain. 2007, 130 (1\u20132): 126-136.","journal-title":"Pain"},{"issue":"1","key":"638_CR29","doi-asserted-by":"publisher","first-page":"3","DOI":"10.1007\/PL00005742","volume":"116","author":"MA Pozo","year":"1997","unstructured":"Pozo MA, Balazs EA, Belmonte C: Reduction of sensory responses to passive movements of inflamed knee joints by hylan, a hyaluronan derivative. Experiment brain Res Experiment Hirnforschung Experiment cerebr. 1997, 116 (1): 3-9. 10.1007\/PL00005742.","journal-title":"Experiment brain Res Experiment Hirnforschung Experiment cerebr"},{"key":"638_CR30","volume-title":"PM & R J Injury Funct Rehab","author":"HK Vincent","year":"2013","unstructured":"Vincent HK, Montero C, Conrad BP, Horodyski M, Connelly J, Martenson M, Seay AN, Vincent KR: \u201cFunctional pain,\u201d functional outcomes, and quality of life after hyaluronic acid intra-articular injection for knee osteoarthritis. PM & R J Injury Funct Rehab. 2013, 10.1016\/j.pmrj.2013.01.004."},{"key":"638_CR31","volume-title":"Modern Rheumatol Japan Rheumatism Assoc","author":"H Oka","year":"2012","unstructured":"Oka H, Akune T, Muraki S, Tanaka S, Kawaguchi H, Nakamura K, Yoshimura N: The mid-term efficacy of intra-articular hyaluronic acid injections on joint structure: a nested case control study. Modern Rheumatol Japan Rheumatism Assoc. 2012, 10.1007\/s10165-012-0725-7."},{"issue":"11","key":"638_CR32","doi-asserted-by":"publisher","first-page":"1336","DOI":"10.1016\/j.joca.2012.07.004","volume":"20","author":"P Li","year":"2012","unstructured":"Li P, Raitcheva D, Hawes M, Moran N, Yu X, Wang F, Matthews GL: Hylan G-F 20 maintains cartilage integrity and decreases osteophyte formation in osteoarthritis through both anabolic and anti-catabolic mechanisms. Osteoarthr cartil OARS Osteoarthr Res Soc. 2012, 20 (11): 1336-1346. 10.1016\/j.joca.2012.07.004.","journal-title":"Osteoarthr cartil OARS Osteoarthr Res Soc"},{"issue":"1\u20132","key":"638_CR33","doi-asserted-by":"publisher","first-page":"285","DOI":"10.1016\/j.ijpharm.2012.11.031","volume":"441","author":"J Dong","year":"2013","unstructured":"Dong J, Jiang D, Wang Z, Wu G, Miao L, Huang L: Intra-articular delivery of liposomal celecoxib-hyaluronate combination for the treatment of osteoarthritis in rabbit model. Int J Pharm. 2013, 441 (1\u20132): 285-290.","journal-title":"Int J Pharm"},{"issue":"1","key":"638_CR34","doi-asserted-by":"publisher","first-page":"R32","DOI":"10.1186\/ar4181","volume":"15","author":"T Naraoka","year":"2013","unstructured":"Naraoka T, Ishibashi Y, Tsuda E, Yamamoto Y, Kusumi T, Toh S: Periodic knee injections of collagen tripeptide delay cartilage degeneration in rabbit experimental osteoarthritis. Arthritis res therapy. 2013, 15 (1): R32-10.1186\/ar4181.","journal-title":"Arthritis res therapy"},{"issue":"2","key":"638_CR35","doi-asserted-by":"publisher","first-page":"99","DOI":"10.1016\/S1063-4584(05)80319-X","volume":"4","author":"T Kikuchi","year":"1996","unstructured":"Kikuchi T, Yamada H, Shimmei M: Effect of high molecular weight hyaluronan on cartilage degeneration in a rabbit model of osteoarthritis. Osteoarthr cartil OARS Osteoarthr Res Soc. 1996, 4 (2): 99-110. 10.1016\/S1063-4584(05)80319-X.","journal-title":"Osteoarthr cartil OARS Osteoarthr Res Soc"},{"issue":"5","key":"638_CR36","doi-asserted-by":"publisher","first-page":"543","DOI":"10.1016\/j.joca.2006.11.001","volume":"15","author":"M Mihara","year":"2007","unstructured":"Mihara M, Higo S, Uchiyama Y, Tanabe K, Saito K: Different effects of high molecular weight sodium hyaluronate and NSAID on the progression of the cartilage degeneration in rabbit OA model. Osteoarthr cartil OARS Osteoarthr Res Soc. 2007, 15 (5): 543-549. 10.1016\/j.joca.2006.11.001.","journal-title":"Osteoarthr cartil OARS Osteoarthr Res Soc"},{"issue":"7","key":"638_CR37","doi-asserted-by":"publisher","first-page":"875","DOI":"10.1002\/art.1780260709","volume":"26","author":"C Colombo","year":"1983","unstructured":"Colombo C, Butler M, O\u2019Byrne E, Hickman L, Swartzendruber D, Selwyn M, Steinetz B: A new model of osteoarthritis in rabbits. I. Development of knee joint pathology following lateral meniscectomy and section of the fibular collateral and sesamoid ligaments. Arthritis rheumatism. 1983, 26 (7): 875-886. 10.1002\/art.1780260709.","journal-title":"Arthritis rheumatism"}],"container-title":["BMC Veterinary Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1186\/1746-6148-9-68.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,6,30]],"date-time":"2023-06-30T22:57:58Z","timestamp":1688165878000},"score":1,"resource":{"primary":{"URL":"https:\/\/bmcvetres.biomedcentral.com\/articles\/10.1186\/1746-6148-9-68"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,4,10]]},"references-count":37,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2013,12]]}},"alternative-id":["638"],"URL":"https:\/\/doi.org\/10.1186\/1746-6148-9-68","relation":{},"ISSN":["1746-6148"],"issn-type":[{"value":"1746-6148","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,4,10]]},"assertion":[{"value":"17 January 2013","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"26 March 2013","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"10 April 2013","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}}],"article-number":"68"}}