{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,1]],"date-time":"2025-11-01T13:38:24Z","timestamp":1762004304731},"reference-count":40,"publisher":"Springer Science and Business Media LLC","issue":"1","content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["J Orthop Surg Res"],"published-print":{"date-parts":[[2010,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n          <jats:sec>\n            <jats:title>Background<\/jats:title>\n            <jats:p>Recent studies demonstrated an impact of the nervous system on particle-induced osteolysis, the major cause of aseptic loosening of joint replacements.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Methods<\/jats:title>\n            <jats:p>In this study of MG-63 osteoblast-like cells we analyzed the influence of ultra-high molecular weight polyethylene (UHMWPE) particles and the neurotransmitter alpha-calcitonin gene-related peptide (CGRP) on the osteoprotegerin\/receptor activator of nuclear factor-\u03baB ligand\/receptor activator of nuclear factor\u03baB (OPG\/RANKL\/RANK) system. MG-63 cells were stimulated by different UHMWPE particle concentrations (1:100, 1:500) and different doses of alpha-CGRP (10<jats:sup>-7<\/jats:sup> M, 10<jats:sup>-9<\/jats:sup> M, 10<jats:sup>-11<\/jats:sup> M). RANKL and OPG mRNA expression and protein levels were measured by RT-PCR and Western blot.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Results<\/jats:title>\n            <jats:p>Increasing particle concentrations caused an up-regulation of RANKL after 72 hours. Alpha-CGRP showed a dose-independent depressive effect on particle-induced expression of RANKL mRNA in both cell-particle ratios. RANKL gene transcripts were significantly (P &lt; 0.05) decreased by alpha-CGRP treatment after 48 and 72 hours. OPG mRNA was significantly down-regulated in a cell-particle ratio of 1:500 after 72 hours. Alpha-CGRP concentrations of 10<jats:sup>-7<\/jats:sup> M lead to an up-regulation of OPG protein.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Conclusion<\/jats:title>\n            <jats:p>In conclusion, a possible osteoprotective influence of the neurotransmitter alpha-CGRP on particle stimulated osteoblast-like cells could be shown. Alpha-CGRP might be important for bone metabolism under conditions of particle-induced osteolysis.<\/jats:p>\n          <\/jats:sec>","DOI":"10.1186\/1749-799x-5-83","type":"journal-article","created":{"date-parts":[[2010,11,5]],"date-time":"2010-11-05T07:37:00Z","timestamp":1288942620000},"update-policy":"http:\/\/dx.doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Effects of alpha-calcitonin gene-related peptide on osteoprotegerin and receptor activator of nuclear factor-\u03baB ligand expression in MG-63 osteoblast-like cells exposed to polyethylene particles"],"prefix":"10.1186","volume":"5","author":[{"given":"Jie","family":"Xu","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Max D","family":"Kauther","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Julia","family":"Hartl","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christian","family":"Wedemeyer","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"name":"Study was performed at the University of Duisburg - Essen, Germany","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2010,11,4]]},"reference":[{"key":"225_CR1","doi-asserted-by":"publisher","first-page":"138","DOI":"10.1097\/00003086-200212000-00016","volume":"405","author":"TW Bauer","year":"2002","unstructured":"Bauer TW: Particles and periimplant bone resorption. Clin Orthop Relat Res. 2002, 405: 138-143. 10.1097\/00003086-200212000-00016.","journal-title":"Clin Orthop Relat Res"},{"key":"225_CR2","doi-asserted-by":"publisher","first-page":"151","DOI":"10.1302\/0301-620X.80B1.8138","volume":"80","author":"M Ahmed","year":"1998","unstructured":"Ahmed M, Bergstrom J, Lundblad H, Gillespie WJ, Kreicbergs A: Sensory nerves in the interface membrane of aseptic loose hip prostheses. J Bone Joint Surg Br. 1998, 80: 151-155. 10.1302\/0301-620X.80B1.8138.","journal-title":"J Bone Joint Surg Br"},{"key":"225_CR3","doi-asserted-by":"publisher","first-page":"342","DOI":"10.1080\/17453670710015238","volume":"79","author":"Y Qian","year":"2008","unstructured":"Qian Y, Zeng BF, Zhang XL, Jiang Y: High levels of substance P and CGRP in pseudosynovial fluid from patients with aseptic loosening of their hip prosthesis. Acta Orthop. 2008, 79: 342-345. 10.1080\/17453670710015238.","journal-title":"Acta Orthop"},{"key":"225_CR4","doi-asserted-by":"publisher","first-page":"1011","DOI":"10.1359\/jbmr.070408","volume":"22","author":"C Wedemeyer","year":"2007","unstructured":"Wedemeyer C, Neuerburg C, Pfeiffer A, Heckelei A, Bylski D, von Knoch F, Schinke T, Hilken G, Gosheger G, von Knoch M: Polyethylene particle-induced bone resorption in alpha-calcitonin gene-related peptide-deficient mice. J Bone Miner Res. 2007, 22: 1011-1019. 10.1359\/jbmr.070408.","journal-title":"J Bone Miner Res"},{"key":"225_CR5","doi-asserted-by":"publisher","first-page":"713","DOI":"10.1111\/j.1476-5381.1995.tb14991.x","volume":"115","author":"K Beaumont","year":"1995","unstructured":"Beaumont K, Pittner RA, Moore CX, Wolfe-Lopez D, Prickett KS, Young AA, Rink TJ: Regulation of muscle glycogen metabolism by CGRP and amylin: CGRP receptors not involved. Br J Pharmacol. 1995, 115: 713-715.","journal-title":"Br J Pharmacol"},{"issue":"Pt 3","key":"225_CR6","doi-asserted-by":"publisher","first-page":"707","DOI":"10.1042\/bj3070707","volume":"307","author":"B Leighton","year":"1995","unstructured":"Leighton B, Foot EA: The role of the sensory peptide calcitonin-gene-related peptide(s) in skeletal muscle carbohydrate metabolism: effects of capsaicin and resiniferatoxin. Biochem J. 1995, 307 (Pt 3): 707-712.","journal-title":"Biochem J"},{"key":"225_CR7","doi-asserted-by":"publisher","first-page":"533","DOI":"10.1210\/edrv-17-5-533","volume":"17","author":"SJ Wimalawansa","year":"1996","unstructured":"Wimalawansa SJ: Calcitonin gene-related peptide and its receptors: molecular genetics, physiology, pathophysiology, and therapeutic potentials. Endocr Rev. 1996, 17: 533-585.","journal-title":"Endocr Rev"},{"key":"225_CR8","doi-asserted-by":"publisher","first-page":"167","DOI":"10.1615\/CritRevNeurobiol.v11.i2-3.40","volume":"11","author":"SJ Wimalawansa","year":"1997","unstructured":"Wimalawansa SJ: Amylin, calcitonin gene-related peptide, calcitonin, and adrenomedullin: a peptide superfamily. Crit Rev Neurobiol. 1997, 11: 167-239.","journal-title":"Crit Rev Neurobiol"},{"key":"225_CR9","doi-asserted-by":"publisher","first-page":"165","DOI":"10.1016\/0196-9781(88)90023-X","volume":"9","author":"A Bjurholm","year":"1988","unstructured":"Bjurholm A, Kreicbergs A, Brodin E, Schultzberg M: Substance P- and CGRP-immunoreactive nerves in bone. Peptides. 1988, 9: 165-171. 10.1016\/0196-9781(88)90023-X.","journal-title":"Peptides"},{"key":"225_CR10","doi-asserted-by":"publisher","first-page":"339","DOI":"10.1007\/BF02555747","volume":"38","author":"I Yamamoto","year":"1986","unstructured":"Yamamoto I, Kitamura N, Aoki J, Shigeno C, Hino M, Asonuma K, Torizuka K, Fujii N, Otaka A, Yajima H: Human calcitonin gene-related peptide possesses weak inhibitory potency of bone resorption in vitro. Calcif Tissue Int. 1986, 38: 339-341. 10.1007\/BF02555747.","journal-title":"Calcif Tissue Int"},{"key":"225_CR11","doi-asserted-by":"publisher","first-page":"855","DOI":"10.1111\/j.1476-5381.1985.tb11107.x","volume":"86","author":"SD Brain","year":"1985","unstructured":"Brain SD, Williams TJ: Inflammatory oedema induced by synergism between calcitonin gene-related peptide (CGRP) and mediators of increased vascular permeability. Br J Pharmacol. 1985, 86: 855-860.","journal-title":"Br J Pharmacol"},{"key":"225_CR12","doi-asserted-by":"publisher","first-page":"5050","DOI":"10.1210\/endo.142.12.8536","volume":"142","author":"S Khosla","year":"2001","unstructured":"Khosla S: Minireview: the OPG\/RANKL\/RANK system. Endocrinology. 2001, 142: 5050-5055. 10.1210\/en.142.12.5050.","journal-title":"Endocrinology"},{"key":"225_CR13","doi-asserted-by":"publisher","first-page":"1329","DOI":"10.1210\/en.139.3.1329","volume":"139","author":"H Yasuda","year":"1998","unstructured":"Yasuda H, Shima N, Nakagawa N, Mochizuki SI, Yano K, Fujise N, Sato Y, Goto M, Yamaguchi K, Kuriyama M: Identity of osteoclastogenesis inhibitory factor (OCIF) and osteoprotegerin (OPG): a mechanism by which OPG\/OCIF inhibits osteoclastogenesis in vitro. Endocrinology. 1998, 139: 1329-1337. 10.1210\/en.139.3.1329.","journal-title":"Endocrinology"},{"key":"225_CR14","doi-asserted-by":"publisher","first-page":"2","DOI":"10.1359\/jbmr.2000.15.1.2","volume":"15","author":"LC Hofbauer","year":"2000","unstructured":"Hofbauer LC, Khosla S, Dunstan CR, Lacey DL, Boyle WJ, Riggs BL: The roles of osteoprotegerin and osteoprotegerin ligand in the paracrine regulation of bone resorption. J Bone Miner Res. 2000, 15: 2-12. 10.1359\/jbmr.2000.15.1.2.","journal-title":"J Bone Miner Res"},{"key":"225_CR15","doi-asserted-by":"publisher","first-page":"452","DOI":"10.1302\/0301-620X.81B3.8758","volume":"81","author":"DD Dean","year":"1999","unstructured":"Dean DD, Schwartz Z, Liu Y, Blanchard CR, Agrawal CM, Mabrey JD, Sylvia VL, Lohmann CH, Boyan BD: The effect of ultra-high molecular weight polyethylene wear debris on MG63 osteosarcoma cells in vitro. J Bone Joint Surg Am. 1999, 81: 452-461. 10.1302\/0301-620X.81B3.8758.","journal-title":"J Bone Joint Surg Am"},{"key":"225_CR16","doi-asserted-by":"publisher","first-page":"C529","DOI":"10.1152\/ajpcell.00354.2005","volume":"291","author":"I Villa","year":"2006","unstructured":"Villa I, Mrak E, Rubinacci A, Ravasi F, Guidobono F: CGRP inhibits osteoprotegerin production in human osteoblast-like cells via cAMP\/PKA-dependent pathway. Am J Physiol Cell Physiol. 2006, 291: C529-537. 10.1152\/ajpcell.00354.2005.","journal-title":"Am J Physiol Cell Physiol"},{"key":"225_CR17","doi-asserted-by":"publisher","first-page":"366","DOI":"10.1055\/s-2004-822589","volume":"142","author":"M von Knoch","year":"2004","unstructured":"von Knoch M, Sprecher C, Barden B, Saxler G, Loer F, Wimmer M: Size and shape of commercially available polyethylene particles for in-vitro and in-vivo-experiments. Z Orthop Ihre Grenzgeb. 2004, 142: 366-370. 10.1055\/s-2004-822589.","journal-title":"Z Orthop Ihre Grenzgeb"},{"key":"225_CR18","doi-asserted-by":"publisher","first-page":"1575","DOI":"10.1002\/jor.20190","volume":"24","author":"W Ren","year":"2006","unstructured":"Ren W, Wu B, Peng X, Hua J, Hao HN, Wooley PH: Implant wear induces inflammation, but not osteoclastic bone resorption, in RANK(-\/-) mice. J Orthop Res. 2006, 24: 1575-1586. 10.1002\/jor.20190.","journal-title":"J Orthop Res"},{"key":"225_CR19","doi-asserted-by":"publisher","first-page":"71","DOI":"10.1097\/00003086-200112000-00008","volume":"393","author":"JJ Jacobs","year":"2001","unstructured":"Jacobs JJ, Roebuck KA, Archibeck M, Hallab NJ, Glant TT: Osteolysis: basic science. Clin Orthop Relat Res. 2001, 393: 71-77. 10.1097\/00003086-200112000-00008.","journal-title":"Clin Orthop Relat Res"},{"key":"225_CR20","doi-asserted-by":"publisher","first-page":"26","DOI":"10.1136\/ard.59.1.26","volume":"59","author":"I Itonaga","year":"2000","unstructured":"Itonaga I, Sabokbar A, Murray DW, Athanasou NA: Effect of osteoprotegerin and osteoprotegerin ligand on osteoclast formation by arthroplasty membrane derived macrophages. Ann Rheum Dis. 2000, 59: 26-31. 10.1136\/ard.59.1.26.","journal-title":"Ann Rheum Dis"},{"key":"225_CR21","doi-asserted-by":"publisher","first-page":"813","DOI":"10.1016\/j.bone.2008.07.003","volume":"43","author":"D Naot","year":"2008","unstructured":"Naot D, Cornish J: The role of peptides and receptors of the calcitonin family in the regulation of bone metabolism. Bone. 2008, 43: 813-818. 10.1016\/j.bone.2008.07.003.","journal-title":"Bone"},{"key":"225_CR22","doi-asserted-by":"publisher","first-page":"1302","DOI":"10.1359\/jbmr.1999.14.8.1302","volume":"14","author":"J Cornish","year":"1999","unstructured":"Cornish J, Callon KE, Lin CQ, Xiao CL, Gamble GD, Cooper GJ, Reid IR: Comparison of the effects of calcitonin gene-related peptide and amylin on osteoblasts. J Bone Miner Res. 1999, 14: 1302-1309. 10.1359\/jbmr.1999.14.8.1302.","journal-title":"J Bone Miner Res"},{"key":"225_CR23","doi-asserted-by":"publisher","first-page":"1067","DOI":"10.1359\/jbmr.1999.14.7.1067","volume":"14","author":"R Ballica","year":"1999","unstructured":"Ballica R, Valentijn K, Khachatryan A, Guerder S, Kapadia S, Gundberg C, Gilligan J, Flavell RA, Vignery A: Targeted expression of calcitonin gene-related peptide to osteoblasts increases bone density in mice. J Bone Miner Res. 1999, 14: 1067-1074. 10.1359\/jbmr.1999.14.7.1067.","journal-title":"J Bone Miner Res"},{"key":"225_CR24","doi-asserted-by":"publisher","first-page":"2049","DOI":"10.1359\/jbmr.040915","volume":"19","author":"T Schinke","year":"2004","unstructured":"Schinke T, Liese S, Priemel M, Haberland M, Schilling AF, Catala-Lehnen P, Blicharski D, Rueger JM, Gagel RF, Emeson RB, Amling M: Decreased bone formation and osteopenia in mice lacking alpha-calcitonin gene-related peptide. J Bone Miner Res. 2004, 19: 2049-2056. 10.1359\/jbmr.040915.","journal-title":"J Bone Miner Res"},{"key":"225_CR25","doi-asserted-by":"publisher","first-page":"559","DOI":"10.1016\/S0196-9781(00)00185-6","volume":"21","author":"A Akopian","year":"2000","unstructured":"Akopian A, Demulder A, Ouriaghli F, Corazza F, Fondu P, Bergmann P: Effects of CGRP on human osteoclast-like cell formation: a possible connection with the bone loss in neurological disorders?. Peptides. 2000, 21: 559-564. 10.1016\/S0196-9781(00)00185-6.","journal-title":"Peptides"},{"key":"225_CR26","doi-asserted-by":"publisher","first-page":"149","DOI":"10.1007\/BF02555699","volume":"40","author":"M Zaidi","year":"1987","unstructured":"Zaidi M, Fuller K, Bevis PJ, GainesDas RE, Chambers TJ, MacIntyre I: Calcitonin gene-related peptide inhibits osteoclastic bone resorption: a comparative study. Calcif Tissue Int. 1987, 40: 149-154. 10.1007\/BF02555699.","journal-title":"Calcif Tissue Int"},{"key":"225_CR27","doi-asserted-by":"publisher","first-page":"1924","DOI":"10.1359\/jbmr.060820","volume":"21","author":"AK Huebner","year":"2006","unstructured":"Huebner AK, Schinke T, Priemel M, Schilling S, Schilling AF, Emeson RB, Rueger JM, Amling M: Calcitonin deficiency in mice progressively results in high bone turnover. J Bone Miner Res. 2006, 21: 1924-1934. 10.1359\/jbmr.060820.","journal-title":"J Bone Miner Res"},{"key":"225_CR28","doi-asserted-by":"publisher","first-page":"162","DOI":"10.1016\/S8756-3282(01)00494-X","volume":"29","author":"J Cornish","year":"2001","unstructured":"Cornish J, Callon KE, Bava U, Kamona SA, Cooper GJ, Reid IR: Effects of calcitonin, amylin, and calcitonin gene-related peptide on osteoclast development. Bone. 2001, 29: 162-168. 10.1016\/S8756-3282(01)00494-X.","journal-title":"Bone"},{"key":"225_CR29","doi-asserted-by":"publisher","first-page":"307","DOI":"10.1016\/S1028-4559(09)60248-5","volume":"45","author":"FP Chen","year":"2006","unstructured":"Chen FP, Hsu T, Hu CH, Wang WD, Wang KC, Teng LF: Expression of estrogen receptors alfa and beta mRNA and alkaline phosphatase in the differentiation of osteoblasts from elderly postmenopausal women: comparison with osteoblasts from osteosarcoma cell lines. Taiwan J Obstet Gynecol. 2006, 45: 307-312. 10.1016\/S1028-4559(09)60248-5.","journal-title":"Taiwan J Obstet Gynecol"},{"key":"225_CR30","doi-asserted-by":"publisher","first-page":"193","DOI":"10.1016\/S1359-6101(03)00021-2","volume":"14","author":"PW Dempsey","year":"2003","unstructured":"Dempsey PW, Doyle SE, He JQ, Cheng G: The signaling adaptors and pathways activated by TNF superfamily. Cytokine Growth Factor Rev. 2003, 14: 193-209. 10.1016\/S1359-6101(03)00021-2.","journal-title":"Cytokine Growth Factor Rev"},{"key":"225_CR31","doi-asserted-by":"publisher","first-page":"788","DOI":"10.2106\/JBJS.E.00711","volume":"88","author":"DS Rakshit","year":"2006","unstructured":"Rakshit DS, Ly K, Sengupta TK, Nestor BJ, Sculco TP, Ivashkiv LB, Purdue PE: Wear debris inhibition of anti-osteoclastogenic signaling by interleukin-6 and interferon-gamma. Mechanistic insights and implications for periprosthetic osteolysis. J Bone Joint Surg Am. 2006, 88: 788-799. 10.2106\/JBJS.E.00711.","journal-title":"J Bone Joint Surg Am"},{"key":"225_CR32","doi-asserted-by":"crossref","first-page":"415S","DOI":"10.1093\/jn\/138.2.415S","volume":"138","author":"TR Arnett","year":"2008","unstructured":"Arnett TR: Extracellular pH regulates bone cell function. J Nutr. 2008, 138: 415S-418S.","journal-title":"J Nutr"},{"key":"225_CR33","doi-asserted-by":"publisher","first-page":"316","DOI":"10.1111\/j.1349-7006.2007.00690.x","volume":"99","author":"S Sato","year":"2008","unstructured":"Sato S, Futakuchi M, Ogawa K, Asamoto M, Nakao K, Asai K, Shirai T: Transforming growth factor beta derived from bone matrix promotes cell proliferation of prostate cancer and osteoclast activation-associated osteolysis in the bone microenvironment. Cancer Sci. 2008, 99: 316-323. 10.1111\/j.1349-7006.2007.00690.x.","journal-title":"Cancer Sci"},{"key":"225_CR34","doi-asserted-by":"publisher","first-page":"457","DOI":"10.1016\/j.cytogfr.2004.06.004","volume":"15","author":"S Theoleyre","year":"2004","unstructured":"Theoleyre S, Wittrant Y, Tat SK, Fortun Y, Redini F, Heymann D: The molecular triad OPG\/RANK\/RANKL: involvement in the orchestration of pathophysiological bone remodeling. Cytokine Growth Factor Rev. 2004, 15: 457-475. 10.1016\/j.cytogfr.2004.06.004.","journal-title":"Cytokine Growth Factor Rev"},{"key":"225_CR35","doi-asserted-by":"publisher","first-page":"582","DOI":"10.1002\/jbm.b.30244","volume":"74","author":"J Mandelin","year":"2005","unstructured":"Mandelin J, Liljestrom M, Li TF, Ainola M, Hukkanen M, Salo J, Santavirta S, Konttinen YT: Pseudosynovial fluid from loosened total hip prosthesis induces osteoclast formation. J Biomed Mater Res B Appl Biomater. 2005, 74: 582-588.","journal-title":"J Biomed Mater Res B Appl Biomater"},{"key":"225_CR36","doi-asserted-by":"publisher","first-page":"383","DOI":"10.1016\/S8756-3282(00)00335-5","volume":"27","author":"MC Langub","year":"2000","unstructured":"Langub MC, Reinhardt TA, Horst RL, Malluche HH, Koszewski NJ: Characterization of vitamin D receptor immunoreactivity in human bone cells. Bone. 2000, 27: 383-387. 10.1016\/S8756-3282(00)00335-5.","journal-title":"Bone"},{"key":"225_CR37","doi-asserted-by":"publisher","first-page":"895","DOI":"10.1139\/O09-052","volume":"87","author":"J Parreno","year":"2009","unstructured":"Parreno J, Hart DA: Molecular and mechano-biology of collagen gel contraction mediated by human MG-63 cells: involvement of specific intracellular signaling pathways and the cytoskeleton. Biochem Cell Biol. 2009, 87: 895-904. 10.1139\/O09-052.","journal-title":"Biochem Cell Biol"},{"key":"225_CR38","first-page":"366","volume":"24","author":"M Wiontzek","year":"2006","unstructured":"Wiontzek M, Matziolis G, Schuchmann S, Gaber T, Krocker D, Duda G, Burmester GR, Perka C, Buttgereit F: Effects of dexamethasone and celecoxib on calcium homeostasis and expression of cyclooxygenase-2 mRNA in MG-63 human osteosarcoma cells. Clin Exp Rheumatol. 2006, 24: 366-372.","journal-title":"Clin Exp Rheumatol"},{"key":"225_CR39","doi-asserted-by":"publisher","first-page":"585","DOI":"10.1016\/8756-3282(94)90305-0","volume":"15","author":"J Clover","year":"1994","unstructured":"Clover J, Gowen M: Are MG-63 and HOS TE85 human osteosarcoma cell lines representative models of the osteoblastic phenotype?. Bone. 1994, 15: 585-591. 10.1016\/8756-3282(94)90305-0.","journal-title":"Bone"},{"key":"225_CR40","doi-asserted-by":"publisher","first-page":"19","DOI":"10.1007\/s00774-003-0442-6","volume":"22","author":"XH Luo","year":"2004","unstructured":"Luo XH, Liao EY, Su X, Wu XP: Parathyroid hormone inhibits the expression of membrane-type matrix metalloproteinase-1 (MT1-MMP) in osteoblast-like MG-63 cells. J Bone Miner Metab. 2004, 22: 19-25. 10.1007\/s00774-003-0442-6.","journal-title":"J Bone Miner Metab"}],"container-title":["Journal of Orthopaedic Surgery and Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1186\/1749-799X-5-83.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,9,1]],"date-time":"2021-09-01T11:28:29Z","timestamp":1630495709000},"score":1,"resource":{"primary":{"URL":"https:\/\/josr-online.biomedcentral.com\/articles\/10.1186\/1749-799X-5-83"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2010,11,4]]},"references-count":40,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2010,12]]}},"alternative-id":["225"],"URL":"https:\/\/doi.org\/10.1186\/1749-799x-5-83","relation":{},"ISSN":["1749-799X"],"issn-type":[{"type":"electronic","value":"1749-799X"}],"subject":[],"published":{"date-parts":[[2010,11,4]]},"assertion":[{"value":"8 April 2010","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"4 November 2010","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"4 November 2010","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}}],"article-number":"83"}}