{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,10]],"date-time":"2025-12-10T15:51:23Z","timestamp":1765381883050},"reference-count":28,"publisher":"Springer Science and Business Media LLC","issue":"1","content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Chin Med"],"published-print":{"date-parts":[[2014,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n          <jats:sec>\n            <jats:title>Background<\/jats:title>\n            <jats:p>Tree bark of <jats:italic>Eucommia ulmoides<\/jats:italic> Oliv., (commonly well-known as \u201c<jats:italic>Du-zhong<\/jats:italic>\u201d in China), has been used to treat hypertension, hypercholesterolemia, hyperglycemia, hepatic fibrosis and renal injury. This study aims to investigate the effects of lignans extracted from the bark of <jats:italic>Eucommia ulmoides<\/jats:italic> Oliv. on Ang II-induced proliferation and extracellular matrix biosynthesis in rat mesangial cells.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Methods<\/jats:title>\n            <jats:p>Rat mesangial cells (RMCs) were cultured <jats:italic>in vitro<\/jats:italic> and divided into six groups (control, Ang II, losartan, and low, middle and high concentration lignans groups). RMC proliferation was measured by MTT assay. RT-qPCR and western blotting were used to detect mRNA and protein expression of collagen type I (Col I), collagen type III (Col III), collagen type IV (Col IV), fibronectin and aldose reductase (AR).<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Results<\/jats:title>\n            <jats:p>Cellular proliferation induced by Ang II was significantly suppressed by <jats:italic>Eucommia<\/jats:italic> lignans of different concentrations (<jats:italic>P<\/jats:italic>\u2009=\u20090.034, <jats:italic>P<\/jats:italic>\u2009&lt;\u20090.001, and <jats:italic>P<\/jats:italic>\u2009&lt;\u20090.001). Treatment of cells with Ang II increased Col I, Col III, Col IV, and fibronectin mRNA expression, which was observed at the protein level (<jats:italic>P\u2009&lt;<\/jats:italic>\u20090.001, <jats:italic>P\u2009&lt;<\/jats:italic>\u20090.001, <jats:italic>P<\/jats:italic>\u2009=\u20090.004, and <jats:italic>P<\/jats:italic>\u2009=\u20090.004, respectively). The increased mRNA expression and protein levels of Col I, Col III, Col IV, and fibronectin were diminished remarkably with by treatment <jats:italic>Eucommia<\/jats:italic> lignans, and elevated AR expression stimulated by Ang II was significantly inhibited by <jats:italic>Eucommia<\/jats:italic> lignans.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Conclusions<\/jats:title>\n            <jats:p>\n              <jats:italic>Eucommia<\/jats:italic> lignans (<jats:italic>Du-zhong<\/jats:italic>) inhibited Ang II-stimulated extracellular matrix biosynthesis in mesangial cells.<\/jats:p>\n          <\/jats:sec>","DOI":"10.1186\/1749-8546-9-8","type":"journal-article","created":{"date-parts":[[2014,2,14]],"date-time":"2014-02-14T04:01:04Z","timestamp":1392350464000},"update-policy":"http:\/\/dx.doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":23,"title":["Lignans from the bark of Eucommia ulmoides inhibited Ang II-stimulated extracellular matrix biosynthesis in mesangial cells"],"prefix":"10.1186","volume":"9","author":[{"given":"Zhen-yu","family":"Li","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiao-lan","family":"Deng","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wei-hua","family":"Huang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ling","family":"Li","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hui","family":"Li","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xian","family":"Jing","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ying-ying","family":"Tian","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pei-yu","family":"Lv","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tian-lun","family":"Yang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hong-hao","family":"Zhou","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dong-sheng","family":"Ouyang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2014,2,14]]},"reference":[{"key":"203_CR1","unstructured":"2009 Annual data report: atlas of end-stage renal disease in the United States. 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