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Regarding the previous results and also few numbers of studies about the presence of HP in benign laryngeal lesions, specifically by PCR, we aimed to investigate the presence of HP in benign laryngeal lesions by in-house PCR.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Methods<\/jats:title>\n            <jats:p>The samples were taken from 55 patients with benign laryngeal lesions and frozen in \u221220\u00b0C. One milliliter (ml) of lysis buffer was added to 100 mg (mg) of each sample and the tube was placed in 56\u00b0C overnight. Then DNA extraction was carried out.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Results<\/jats:title>\n            <jats:p>To find HP DNA, in-house PCR was performed that revealed 5 positive results among 55 patients with benign laryngeal lesions. Of them, 3 were polyp, 1 was nodule and 1 was papilloma.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Conclusion<\/jats:title>\n            <jats:p>Although the number of positive results was not a lot in this study, it was in contrast with previous studies which could not find any HP tracks in benign laryngeal lesions by other methods. More studies about the prevalence of HP in benign laryngeal lesions improve judging about the effect of this infection on benign laryngeal lesions.<\/jats:p>\n          <\/jats:sec>","DOI":"10.1186\/1750-9378-7-10","type":"journal-article","created":{"date-parts":[[2012,4,19]],"date-time":"2012-04-19T16:14:23Z","timestamp":1334852063000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Detection of helicobacter pylori in benign laryngeal lesions by polymerase chain reaction: a cross sectional study"],"prefix":"10.1186","volume":"7","author":[{"given":"Farzad","family":"Izadi","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aslan","family":"Ahmadi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shadi","family":"Ghourchian","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ahmad","family":"Daneshi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Faramarz","family":"Memari","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ehsan","family":"Khadivi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shabahang","family":"Mohammadi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2012,4,19]]},"reference":[{"issue":"5","key":"389_CR1","doi-asserted-by":"publisher","first-page":"618","DOI":"10.1016\/j.canep.2010.05.005","volume":"34","author":"AR Andr\u00e9","year":"2010","unstructured":"Andr\u00e9 AR, Ferreira MV, Mota RM, Ferrasi AC, Pardini MI, Rabenhorst SH: Gastric adenocarcinoma and Helicobacter pylori: correlation with p53 mutation and p27 immunoexpression. 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